TY - JOUR
T1 - Use of iQPR-H2O for bone regeneration and its potential in the improvement of osteoporosis
AU - Lee, Chi-Ming
AU - Cheong, Meileng
AU - Hsiao, Wentien
AU - Liu, Henyu
AU - Tsai, Chingyu
AU - Wang, Mingfu
AU - Wu, Chihhsiung
AU - Chang, Kwanghwa
AU - Lam, Gowlin
AU - Deng, Winping
PY - 2011
Y1 - 2011
N2 - Background: Current treatments for osteoporosis are associated with various side effects and do not prevent the age-related decrease in osteoblast number. The objective of this study was to evaluate the effects of iQPR-H2O on osteogenesis. Methods. Mouse fibroblast NIH3T3 and pre-osteoblastic MC3T3-E1 cells were cultured in medium prepared with iQPR-H2O or unprocessed mineral water (control cells), and proliferation and differentiation were assessed by MTT and alkaline phosphatase assay, respectively. Mineral deposition by the cells was determined using Alizarin red S staining. A mouse model of osteoporosis, ovariectomized SAMP8 mice, was used to evaluate the effects of iQPR-H2O on osteogenesis in vivo. Mice were given either iQPR-H 2O or unprocessed mineral water (control group) for four months after which bone mass density (BMD) measurements were made using a bone densitometer and hematoxylin and eosin staining of bone samples. Results: NIH3T3 cells grown in medium prepared with iQPR-H2O exhibited significantly greater proliferation. NIH3T3 and MC3T3-E1 cells demonstrated a significant increase in alkaline phosphatase levels in the iQPR-H2O group. MC3T3-E1 cells showed mineralization at day 28. mRNA expression levels of both osteopontin and runt-related transcription factor 2 in MC3T3-E1 cells were higher in the iQPR-H2O group compared with the control group. After four months, significantly greater bone regeneration was evident in ovariectomized SAMP8 mice administered iQPR-H2O as compared with control group. Conclusions: iQPR-H2O may reduce the symptoms of osteoporosis by improving osteogenesis.
AB - Background: Current treatments for osteoporosis are associated with various side effects and do not prevent the age-related decrease in osteoblast number. The objective of this study was to evaluate the effects of iQPR-H2O on osteogenesis. Methods. Mouse fibroblast NIH3T3 and pre-osteoblastic MC3T3-E1 cells were cultured in medium prepared with iQPR-H2O or unprocessed mineral water (control cells), and proliferation and differentiation were assessed by MTT and alkaline phosphatase assay, respectively. Mineral deposition by the cells was determined using Alizarin red S staining. A mouse model of osteoporosis, ovariectomized SAMP8 mice, was used to evaluate the effects of iQPR-H2O on osteogenesis in vivo. Mice were given either iQPR-H 2O or unprocessed mineral water (control group) for four months after which bone mass density (BMD) measurements were made using a bone densitometer and hematoxylin and eosin staining of bone samples. Results: NIH3T3 cells grown in medium prepared with iQPR-H2O exhibited significantly greater proliferation. NIH3T3 and MC3T3-E1 cells demonstrated a significant increase in alkaline phosphatase levels in the iQPR-H2O group. MC3T3-E1 cells showed mineralization at day 28. mRNA expression levels of both osteopontin and runt-related transcription factor 2 in MC3T3-E1 cells were higher in the iQPR-H2O group compared with the control group. After four months, significantly greater bone regeneration was evident in ovariectomized SAMP8 mice administered iQPR-H2O as compared with control group. Conclusions: iQPR-H2O may reduce the symptoms of osteoporosis by improving osteogenesis.
KW - Quantum Persistent Reflection
KW - bone mass density
KW - mouse fibroblasts
KW - osteoporosis
KW - senescence-accelerated mice
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U2 - 10.1186/1471-2474-12-227
DO - 10.1186/1471-2474-12-227
M3 - Article
C2 - 21981964
AN - SCOPUS:80053577420
SN - 1471-2474
VL - 12
JO - BMC Musculoskeletal Disorders
JF - BMC Musculoskeletal Disorders
M1 - 227
ER -