Abstract
Urinary d-lactate is highly correlated to diabetic nephropathy - a progressive kidney disease in renal glomeruli. In this study, we used a C3H/3e mouse model to investigate the relationship between urinary d-lactate and aristolochic acid nephropathy where the glomerular structure is not affected. The nephropathy was induced using intravenous injections of aristolochic acid at a dosage of 10mg/kg per day for 5days and was characterized biochemically and histologically. The urinary excretions of proteins, N-acetyl-β-d-glucosaminidase and serum creatinine were determined and connected to histological conventional findings. Urinary d-lactate was analyzed using column-switching high-performance liquid chromatography with fluorescence detection. The results showed a remarkable increase of urinary markers, including of urinary proteins and N-acetyl-β-d-glucosaminidase, and the histological examination confirmed a diagnosis of acute tubule necrosis. The ratio of d-lactate to creatinine in the urine of aristolochic acid-treated mice was approximately 36 times greater than that of the mice in the control group (p<0.05). The ratios for the two groups of mice were 311.00±71.70 and 8.60±1.80μmol/mmol creatinine, respectively. These data confirm in vivo that urinary d-lactate reflects renal injury conditions in aristolochic acid-treated mice and may be a marker for the assessment of nephropathy.
| Original language | English |
|---|---|
| Pages (from-to) | 1100-1106 |
| Number of pages | 7 |
| Journal | Biomedical Chromatography |
| Volume | 27 |
| Issue number | 9 |
| DOIs | |
| Publication status | Published - Sept 2013 |
Keywords
- Aristolochic acid nephropathy
- Renal injury
- Urine
- d-lactate
- l-lactate
ASJC Scopus subject areas
- Drug Discovery
- Analytical Chemistry
- Molecular Biology
- Biochemistry
- Clinical Biochemistry
- Pharmacology
