Up-regulation of interleukin-17 expression by human papillomavirus type 16 E6 in nonsmall cell lung cancer

Yih Hsin Chang, Chen Wei Yu, Li Chuan Lai, Chang Hui Tsao, Kuo Ting Ho, Shun Chun Yang, Huei Lee, Ya Wen Cheng, Tzu Chin Wu, Ming Yuh Shiau

Research output: Contribution to journalArticlepeer-review

35 Citations (Scopus)


BACKGROUND: Human papillomavirus (HPV) 16/18 infection is associated with nonsmoking lung cancer. In this study, the authors investigated a putative correlation between interleukin (IL)-17 expression and HPV infection in clinical nonsmall cell lung cancer (NSCLC) tissues and examined the effects of HPV infection on a human NSCLC cell line. METHODS: IL-17 expression was investigated in 79 NSCLC tumor tissues by immunohistochemistry. Growth rate, IL-17 mRNA, and secreting protein levels were also examined in HPV 16/18 E6-transfected H1299 human NSCLC cells. RESULTS: Immunohistochemical data showed that 48.1% of lung tumors had IL-17 staining, which was significantly associated with patients' sex (P=.03), HPV infection (P=.002), and tumor stage (P=.03). Significant correlations of IL-17 with IL-6 (P <.001) and IL-17 with Mcl-1 (P <.001) expression were also observed. Cell growth rate was increased, and IL-17/Mcl-1 expression levels were elevated in HPV 16 E6-transfected H1299 cells. The transfected E6 oncoproteins can significantly up-regulate expression levels of IL-17 and antiapoptotic protein Mcl-1. CONCLUSIONS: The study suggests that HPV infection-induced IL-17 levels can stimulate Mcl-1 expression through the PI3K pathway and promote lung tumor cell progression through a p53-and IL-6-independent pathway. © 2010 American Cancer Society.

Original languageEnglish
Pages (from-to)4800-4809
Number of pages10
Issue number20
Publication statusPublished - 2010
Externally publishedYes


  • Human papillomavirus
  • Human papillomavirus 16 E6
  • Interleukin-17
  • Mcl-1
  • Nonsmall cell lung cancer

ASJC Scopus subject areas

  • Oncology
  • Cancer Research


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