TY - GEN
T1 - Ultra sensitive detection of eneterovirus 71 by modified electrochemical impedance spectroscopy
AU - Tseng, Shin Hua
AU - Tseng, Dion T.
AU - Lee, Tzu Cheng
AU - Cheng, Tsai Mu
AU - Yang, Jyh Yuan
AU - Hsieh, Ruo Yu
AU - Tsai, Chuan Mei
AU - Chang, Chia Ching
PY - 2010
Y1 - 2010
N2 - The enterovirus 71 (EV71) has threatened Taiwan for more than ten years. Since traditional diagnostic methods are complicated, time consuming, and high-qualified personnel required. Therefore, a new detection process is highly desired. In this study, a high sensitive PC-based electrochemical analyzing system with a functionalized nano-gold modified immunological electrode are developed to detect EV71. Immobilizing specific EV71 polyclone antibodies, which is developed by center for disease control (CDC) Taiwan, onto nano gold of sensing electrode, the affinity interaction of the immobilized antibody with the specific antigen is identified quickly by electrochemical impedance spectrum (EIS) within 20 minutes. The detection limit of this EIS analysis was as low as 50 copy per ml (∼sub-atto molar). In summary, a biosensor and analyzing system based on EIS has been developed to identify EV71 with efficiency, high sensitivity and specificity.
AB - The enterovirus 71 (EV71) has threatened Taiwan for more than ten years. Since traditional diagnostic methods are complicated, time consuming, and high-qualified personnel required. Therefore, a new detection process is highly desired. In this study, a high sensitive PC-based electrochemical analyzing system with a functionalized nano-gold modified immunological electrode are developed to detect EV71. Immobilizing specific EV71 polyclone antibodies, which is developed by center for disease control (CDC) Taiwan, onto nano gold of sensing electrode, the affinity interaction of the immobilized antibody with the specific antigen is identified quickly by electrochemical impedance spectrum (EIS) within 20 minutes. The detection limit of this EIS analysis was as low as 50 copy per ml (∼sub-atto molar). In summary, a biosensor and analyzing system based on EIS has been developed to identify EV71 with efficiency, high sensitivity and specificity.
UR - http://www.scopus.com/inward/record.url?scp=77955070306&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=77955070306&partnerID=8YFLogxK
U2 - 10.1115/nemb2010-13143
DO - 10.1115/nemb2010-13143
M3 - Conference contribution
AN - SCOPUS:77955070306
SN - 9780791843925
T3 - Proceedings of the ASME 1st Global Congress on NanoEngineering for Medicine and Biology 2010, NEMB2010
SP - 27
EP - 28
BT - Proceedings of the ASME 1st Global Congress on NanoEngineering for Medicine and Biology 2010, NEMB2010
PB - ASME
T2 - 1st Global Congress on NanoEngineering for Medicine and Biology: Advancing Health Care through NanoEngineering and Computing, NEMB 2010
Y2 - 7 February 2010 through 10 February 2010
ER -