Type-3 hyaluronan synthase attenuates tumor cells invasion in human mammary parenchymal tissues

Wen Jui Lee; Shih Hsin Tu; Tzu Chun Cheng; Juo Han Lin; Ming Thau Sheu; Ching Chuan Kuo; Chun A. Changou; Chih Hsiung Wu; Hui Wen Chang; Hang Lung Chang; Li Ching Chen; Yuan Soon Ho

doi:10.3390/molecules26216548

Type-3 hyaluronan synthase attenuates tumor cells invasion in human mammary parenchymal tissues

Wen Jui Lee, Shih Hsin Tu, Tzu Chun Cheng, Juo Han Lin, Ming Thau Sheu, Ching Chuan Kuo, Chun A. Changou, Chih Hsiung Wu, Hui Wen Chang, Hang Lung Chang, Li Ching Chen, Yuan Soon Ho

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

The microenvironment for tumor growth and developing metastasis should be essential. This study demonstrated that the hyaluronic acid synthase 3 (HAS3) protein and its enzymatic product hyaluronic acid (HA) encompassed in the subcutaneous extracellular matrix can attenuate the invasion of human breast tumor cells. Decreased HA levels in subcutaneous Has3-KO mouse tissues promoted orthotopic breast cancer (E0771) cell-derived allograft tumor growth. MDA-MB-231 cells premixed with higher concentration HA attenuate tumor growth in xenografted nude mice. Human patient-derived xenotransplantation (PDX) experiments found that HA selected the highly migratory breast cancer cells with CD44 expression accumulated in the tumor/stroma junc-tion. In conclusion, HAS3 and HA were detected in the stroma breast tissues at a high level attenuates effects for induced breast cancer cell death, and inhibit the cancer cells invasion at the initial stage. However, the highly migratory cancer cells were resistant to the HA-mediated effects with unknown mechanisms.

Original language	English
Article number	6548
Journal	Molecules
Volume	26
Issue number	21
DOIs	https://doi.org/10.3390/molecules26216548
Publication status	Published - Nov 1 2021

Keywords

Au-tophagy
Breast cancer
Hyaluronan synthase 3
Tubulin acetylation
Tumor microenvironment

ASJC Scopus subject areas

Analytical Chemistry
Chemistry (miscellaneous)
Molecular Medicine
Pharmaceutical Science
Drug Discovery
Physical and Theoretical Chemistry
Organic Chemistry

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.3390/molecules26216548

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@article{818ad964c7d24f828b9c1a262cc67875,

title = "Type-3 hyaluronan synthase attenuates tumor cells invasion in human mammary parenchymal tissues",

abstract = "The microenvironment for tumor growth and developing metastasis should be essential. This study demonstrated that the hyaluronic acid synthase 3 (HAS3) protein and its enzymatic product hyaluronic acid (HA) encompassed in the subcutaneous extracellular matrix can attenuate the invasion of human breast tumor cells. Decreased HA levels in subcutaneous Has3-KO mouse tissues promoted orthotopic breast cancer (E0771) cell-derived allograft tumor growth. MDA-MB-231 cells premixed with higher concentration HA attenuate tumor growth in xenografted nude mice. Human patient-derived xenotransplantation (PDX) experiments found that HA selected the highly migratory breast cancer cells with CD44 expression accumulated in the tumor/stroma junc-tion. In conclusion, HAS3 and HA were detected in the stroma breast tissues at a high level attenuates effects for induced breast cancer cell death, and inhibit the cancer cells invasion at the initial stage. However, the highly migratory cancer cells were resistant to the HA-mediated effects with unknown mechanisms.",

keywords = "Au-tophagy, Breast cancer, Hyaluronan synthase 3, Tubulin acetylation, Tumor microenvironment",

author = "Lee, {Wen Jui} and Tu, {Shih Hsin} and Cheng, {Tzu Chun} and Lin, {Juo Han} and Sheu, {Ming Thau} and Kuo, {Ching Chuan} and Changou, {Chun A.} and Wu, {Chih Hsiung} and Chang, {Hui Wen} and Chang, {Hang Lung} and Chen, {Li Ching} and Ho, {Yuan Soon}",

note = "Funding Information: Funding: This study was supported by a Health and Welfare Surcharge on Tobacco Products grant (MOHW110-TDU-B-212-144014); the TMU Research Center of Cancer Translational Medicine from The Featured Areas Research Center Program within the framework of the Higher Education Sprout Project by the Ministry of Education (MOE) in Taiwan; and the Ministry of Science and Technology, Taiwan (MOST108-2320-B-038-033-MY3 and MOST110-2320-B-038-082 awarded to Ho, MOST109-2314-B-038-033-MY3 awarded to Tu, MOST110-2320-B-038-031 awarded to Chen). This work was supported by the Zhejiang Innovation Discipline Project of Laboratory Animal Genetic Engineering (No. 201604). Publisher Copyright: {\textcopyright} 2021 by the authors. Licensee MDPI, Basel, Switzerland.",

year = "2021",

month = nov,

day = "1",

doi = "10.3390/molecules26216548",

language = "English",

volume = "26",

journal = "Molecules",

issn = "1420-3049",

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TY - JOUR

T1 - Type-3 hyaluronan synthase attenuates tumor cells invasion in human mammary parenchymal tissues

AU - Lee, Wen Jui

AU - Tu, Shih Hsin

AU - Cheng, Tzu Chun

AU - Lin, Juo Han

AU - Sheu, Ming Thau

AU - Kuo, Ching Chuan

AU - Changou, Chun A.

AU - Wu, Chih Hsiung

AU - Chang, Hui Wen

AU - Chang, Hang Lung

AU - Chen, Li Ching

AU - Ho, Yuan Soon

N1 - Funding Information: Funding: This study was supported by a Health and Welfare Surcharge on Tobacco Products grant (MOHW110-TDU-B-212-144014); the TMU Research Center of Cancer Translational Medicine from The Featured Areas Research Center Program within the framework of the Higher Education Sprout Project by the Ministry of Education (MOE) in Taiwan; and the Ministry of Science and Technology, Taiwan (MOST108-2320-B-038-033-MY3 and MOST110-2320-B-038-082 awarded to Ho, MOST109-2314-B-038-033-MY3 awarded to Tu, MOST110-2320-B-038-031 awarded to Chen). This work was supported by the Zhejiang Innovation Discipline Project of Laboratory Animal Genetic Engineering (No. 201604). Publisher Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland.

PY - 2021/11/1

Y1 - 2021/11/1

N2 - The microenvironment for tumor growth and developing metastasis should be essential. This study demonstrated that the hyaluronic acid synthase 3 (HAS3) protein and its enzymatic product hyaluronic acid (HA) encompassed in the subcutaneous extracellular matrix can attenuate the invasion of human breast tumor cells. Decreased HA levels in subcutaneous Has3-KO mouse tissues promoted orthotopic breast cancer (E0771) cell-derived allograft tumor growth. MDA-MB-231 cells premixed with higher concentration HA attenuate tumor growth in xenografted nude mice. Human patient-derived xenotransplantation (PDX) experiments found that HA selected the highly migratory breast cancer cells with CD44 expression accumulated in the tumor/stroma junc-tion. In conclusion, HAS3 and HA were detected in the stroma breast tissues at a high level attenuates effects for induced breast cancer cell death, and inhibit the cancer cells invasion at the initial stage. However, the highly migratory cancer cells were resistant to the HA-mediated effects with unknown mechanisms.

AB - The microenvironment for tumor growth and developing metastasis should be essential. This study demonstrated that the hyaluronic acid synthase 3 (HAS3) protein and its enzymatic product hyaluronic acid (HA) encompassed in the subcutaneous extracellular matrix can attenuate the invasion of human breast tumor cells. Decreased HA levels in subcutaneous Has3-KO mouse tissues promoted orthotopic breast cancer (E0771) cell-derived allograft tumor growth. MDA-MB-231 cells premixed with higher concentration HA attenuate tumor growth in xenografted nude mice. Human patient-derived xenotransplantation (PDX) experiments found that HA selected the highly migratory breast cancer cells with CD44 expression accumulated in the tumor/stroma junc-tion. In conclusion, HAS3 and HA were detected in the stroma breast tissues at a high level attenuates effects for induced breast cancer cell death, and inhibit the cancer cells invasion at the initial stage. However, the highly migratory cancer cells were resistant to the HA-mediated effects with unknown mechanisms.

KW - Au-tophagy

KW - Breast cancer

KW - Hyaluronan synthase 3

KW - Tubulin acetylation

KW - Tumor microenvironment

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UR - http://www.scopus.com/inward/citedby.url?scp=85118405614&partnerID=8YFLogxK

U2 - 10.3390/molecules26216548

DO - 10.3390/molecules26216548

M3 - Article

AN - SCOPUS:85118405614

SN - 1420-3049

VL - 26

JO - Molecules

JF - Molecules

IS - 21

M1 - 6548

ER -

Type-3 hyaluronan synthase attenuates tumor cells invasion in human mammary parenchymal tissues

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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