Type-3 hyaluronan synthase attenuates tumor cells invasion in human mammary parenchymal tissues

Wen Jui Lee, Shih Hsin Tu, Tzu Chun Cheng, Juo Han Lin, Ming Thau Sheu, Ching Chuan Kuo, Chun A. Changou, Chih Hsiung Wu, Hui Wen Chang, Hang Lung Chang, Li Ching Chen, Yuan Soon Ho

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)


The microenvironment for tumor growth and developing metastasis should be essential. This study demonstrated that the hyaluronic acid synthase 3 (HAS3) protein and its enzymatic product hyaluronic acid (HA) encompassed in the subcutaneous extracellular matrix can attenuate the invasion of human breast tumor cells. Decreased HA levels in subcutaneous Has3-KO mouse tissues promoted orthotopic breast cancer (E0771) cell-derived allograft tumor growth. MDA-MB-231 cells premixed with higher concentration HA attenuate tumor growth in xenografted nude mice. Human patient-derived xenotransplantation (PDX) experiments found that HA selected the highly migratory breast cancer cells with CD44 expression accumulated in the tumor/stroma junc-tion. In conclusion, HAS3 and HA were detected in the stroma breast tissues at a high level attenuates effects for induced breast cancer cell death, and inhibit the cancer cells invasion at the initial stage. However, the highly migratory cancer cells were resistant to the HA-mediated effects with unknown mechanisms.

Original languageEnglish
Article number6548
Issue number21
Publication statusPublished - Nov 1 2021


  • Au-tophagy
  • Breast cancer
  • Hyaluronan synthase 3
  • Tubulin acetylation
  • Tumor microenvironment

ASJC Scopus subject areas

  • Analytical Chemistry
  • Chemistry (miscellaneous)
  • Molecular Medicine
  • Pharmaceutical Science
  • Drug Discovery
  • Physical and Theoretical Chemistry
  • Organic Chemistry


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