Two different roles of purified CD45+c-Kit+Sca-1 +Lin- cells after transplantation in muscles

Momoko Yoshimoto, Hsi Chang, Mitsutaka Shiota, Hirohiko Kobayashi, Katsutsugu Umeda, Atsushi Kawakami, Toshio Heike, Tatsutoshi Nakahata

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)


Recent studies have indicated that bone marrow cells can regenerate damaged muscles and that they can adopt phenotypes of other cells by cell fusion. Our direct visualization system gave evidence of massive muscle regeneration by green fluorescent protein (GFP)-labeled CD45+c-Kit +Sca-1+Lin- cells (KSL cells), and we investigated the role of KSL cells in muscle regeneration after transplantation with or without lethal irradiation. In the early phase, GFP signals were clearly observed in all the muscles of only irradiated mice. Transverse cryostat sections showed GFP+myosin+ muscle fibers, along with numerous GFP+ hematopoietic cells in damaged muscle. These phenomena were temporary, and GFP signals had dramatically reduced 30 days after transplantation. After 6 months, GFP+ fibers could hardly be detected, but GFP+c-Met+ mononuclear cells were located beneath the basal lamina where satellite cells usually exist in both conditioned mice. Immunostaining of isolated single fibers revealed GFP +PAX7+, GFP+MyoD+, and GFP +Myf5+ satellite-like cells on the fibers. Single-fiber cultures from these mice showed proliferation of GFP+ fibers. These results indicate two different roles of KSL cells: one leading to regeneration of damaged muscles in the early phase and the other to conversion into satellite cells in the late phase.

Original languageEnglish
Pages (from-to)610-618
Number of pages9
JournalStem Cells
Issue number5
Publication statusPublished - May 2005
Externally publishedYes


  • Hematopoietic stem cells
  • Muscle stem cells
  • Transplantation
  • c-KitSca-1 Lin

ASJC Scopus subject areas

  • Medicine(all)


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