TY - JOUR
T1 - Tumor induction by direct injection of cloned v-src DNA into chickens
AU - Fung, Y. K T
AU - Crittenden, L. B.
AU - Fadly, A. M.
AU - Kung, H. J.
PY - 1983/1/1
Y1 - 1983/1/1
N2 - The in vitro transforming potential of Rous sarcoma viruses has been shown to reside in a gene segment, v-src. To provide direct proof that the isolated v-src sequence contains all the information necessary for sarcoma induction in vivo, we have injected subgenomic and genomic cloned DNA carrying v-src directly into chickens. We report that v-src, without the complements of other viral genes, can effectively induce sarcomas in the host within weeks after injection. The sarcoma tumors generated by subgenomic v-src DNA are of mono- or oligoclonal origin. They have acquired v-src sequences in the genome and expressed high levels of src messages. Intact long terminal repeats do not seem to be essential in the tumorigenic process.
AB - The in vitro transforming potential of Rous sarcoma viruses has been shown to reside in a gene segment, v-src. To provide direct proof that the isolated v-src sequence contains all the information necessary for sarcoma induction in vivo, we have injected subgenomic and genomic cloned DNA carrying v-src directly into chickens. We report that v-src, without the complements of other viral genes, can effectively induce sarcomas in the host within weeks after injection. The sarcoma tumors generated by subgenomic v-src DNA are of mono- or oligoclonal origin. They have acquired v-src sequences in the genome and expressed high levels of src messages. Intact long terminal repeats do not seem to be essential in the tumorigenic process.
UR - http://www.scopus.com/inward/record.url?scp=0002656278&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0002656278&partnerID=8YFLogxK
U2 - 10.1073/pnas.80.2.353
DO - 10.1073/pnas.80.2.353
M3 - Article
C2 - 6300833
AN - SCOPUS:0002656278
SN - 0027-8424
VL - 80
SP - 353
EP - 357
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 2 I
ER -