Tumor induction by direct injection of cloned v-src DNA into chickens

Y. K T Fung; L. B. Crittenden; A. M. Fadly; H. J. Kung

doi:10.1073/pnas.80.2.353

Tumor induction by direct injection of cloned v-src DNA into chickens

Y. K T Fung, L. B. Crittenden, A. M. Fadly, H. J. Kung

Research output: Contribution to journal › Article › peer-review

53 Citations (Scopus)

Abstract

The in vitro transforming potential of Rous sarcoma viruses has been shown to reside in a gene segment, v-src. To provide direct proof that the isolated v-src sequence contains all the information necessary for sarcoma induction in vivo, we have injected subgenomic and genomic cloned DNA carrying v-src directly into chickens. We report that v-src, without the complements of other viral genes, can effectively induce sarcomas in the host within weeks after injection. The sarcoma tumors generated by subgenomic v-src DNA are of mono- or oligoclonal origin. They have acquired v-src sequences in the genome and expressed high levels of src messages. Intact long terminal repeats do not seem to be essential in the tumorigenic process.

Original language	English
Pages (from-to)	353-357
Number of pages	5
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	80
Issue number	2 I
DOIs	https://doi.org/10.1073/pnas.80.2.353
Publication status	Published - Jan 1 1983
Externally published	Yes

ASJC Scopus subject areas

General
Genetics

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title = "Tumor induction by direct injection of cloned v-src DNA into chickens",

abstract = "The in vitro transforming potential of Rous sarcoma viruses has been shown to reside in a gene segment, v-src. To provide direct proof that the isolated v-src sequence contains all the information necessary for sarcoma induction in vivo, we have injected subgenomic and genomic cloned DNA carrying v-src directly into chickens. We report that v-src, without the complements of other viral genes, can effectively induce sarcomas in the host within weeks after injection. The sarcoma tumors generated by subgenomic v-src DNA are of mono- or oligoclonal origin. They have acquired v-src sequences in the genome and expressed high levels of src messages. Intact long terminal repeats do not seem to be essential in the tumorigenic process.",

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AU - Fung, Y. K T

AU - Crittenden, L. B.

AU - Fadly, A. M.

AU - Kung, H. J.

PY - 1983/1/1

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N2 - The in vitro transforming potential of Rous sarcoma viruses has been shown to reside in a gene segment, v-src. To provide direct proof that the isolated v-src sequence contains all the information necessary for sarcoma induction in vivo, we have injected subgenomic and genomic cloned DNA carrying v-src directly into chickens. We report that v-src, without the complements of other viral genes, can effectively induce sarcomas in the host within weeks after injection. The sarcoma tumors generated by subgenomic v-src DNA are of mono- or oligoclonal origin. They have acquired v-src sequences in the genome and expressed high levels of src messages. Intact long terminal repeats do not seem to be essential in the tumorigenic process.

AB - The in vitro transforming potential of Rous sarcoma viruses has been shown to reside in a gene segment, v-src. To provide direct proof that the isolated v-src sequence contains all the information necessary for sarcoma induction in vivo, we have injected subgenomic and genomic cloned DNA carrying v-src directly into chickens. We report that v-src, without the complements of other viral genes, can effectively induce sarcomas in the host within weeks after injection. The sarcoma tumors generated by subgenomic v-src DNA are of mono- or oligoclonal origin. They have acquired v-src sequences in the genome and expressed high levels of src messages. Intact long terminal repeats do not seem to be essential in the tumorigenic process.

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Tumor induction by direct injection of cloned v-src DNA into chickens

Abstract

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