Triiodothyronine stimulates prostacyclin production by rat aortic smooth muscle cells in culture

Junko Nakao; Wen Chang Chang; Sei Itsu Murota; Hajime Orimo

doi:10.1016/0021-9150(81)90001-0

Triiodothyronine stimulates prostacyclin production by rat aortic smooth muscle cells in culture

Junko Nakao, Wen Chang Chang, Sei Itsu Murota, Hajime Orimo

Research output: Contribution to journal › Article › peer-review

14 Citations (Scopus)

Abstract

The effect of triiodothyronine (T₃) on prostacyclin production in rat aortic smooth muscle cells was investigated by using an intact cell assay system. T₃ at its physiological concentrations has no significant effect on smooth muscle cell proliferation, but it does significantly stimulate prostacyclin production by the cells. Maximum stimulation of prostacyclin production is obtained when cells are treated with T₃ for 4 consecutive days. The dose-response curve shows a linear relationship between the stimulation of prostacyclin production and T₃ concentrations in the range 0.007-10 μg/dl. The maximal prostacyclin production by T₃-treated cells (at a concentration of 10 μg/dl), is 270% compared with control cells. T₃ treatment shows no significant effect on phospholipase (and/or lipase) activities. Our results suggest that thyroid hormone might play an important physiological role in the protection of arteries from atherosclerotic changes by stimulating prostacyclin production in arterial smooth muscle cells.

Original language	English
Pages (from-to)	439-445
Number of pages	7
Journal	Atherosclerosis
Volume	39
Issue number	4
DOIs	https://doi.org/10.1016/0021-9150(81)90001-0
Publication status	Published - Jul 1981
Externally published	Yes

Keywords

Aortic smooth muscle cells
Atherosclerosis
Prostacyclin
Triiodothyronine

ASJC Scopus subject areas

Cardiology and Cardiovascular Medicine

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10.1016/0021-9150(81)90001-0

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title = "Triiodothyronine stimulates prostacyclin production by rat aortic smooth muscle cells in culture",

abstract = "The effect of triiodothyronine (T3) on prostacyclin production in rat aortic smooth muscle cells was investigated by using an intact cell assay system. T3 at its physiological concentrations has no significant effect on smooth muscle cell proliferation, but it does significantly stimulate prostacyclin production by the cells. Maximum stimulation of prostacyclin production is obtained when cells are treated with T3 for 4 consecutive days. The dose-response curve shows a linear relationship between the stimulation of prostacyclin production and T3 concentrations in the range 0.007-10 μg/dl. The maximal prostacyclin production by T3-treated cells (at a concentration of 10 μg/dl), is 270% compared with control cells. T3 treatment shows no significant effect on phospholipase (and/or lipase) activities. Our results suggest that thyroid hormone might play an important physiological role in the protection of arteries from atherosclerotic changes by stimulating prostacyclin production in arterial smooth muscle cells.",

keywords = "Aortic smooth muscle cells, Atherosclerosis, Prostacyclin, Triiodothyronine",

author = "Junko Nakao and Chang, {Wen Chang} and Murota, {Sei Itsu} and Hajime Orimo",

note = "Funding Information: The authors wish to thank Mrs. Y. Kotera for her highly skillful technical assistance and Ono Pharmaceutical Co. Ltd., Oosaka, Japan, for the generous gifts of standard prostaglandins and thromboxane Bz and the financial support for W.C. Chang. Thanks are also due to Dr. K.Yu. of the Yale University School of Medicine, New Haven, CN, U.S.A., for reading the manuscript.",

year = "1981",

month = jul,

doi = "10.1016/0021-9150(81)90001-0",

language = "English",

volume = "39",

pages = "439--445",

journal = "Atherosclerosis",

issn = "0021-9150",

publisher = "Elsevier Ireland Ltd",

number = "4",

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T1 - Triiodothyronine stimulates prostacyclin production by rat aortic smooth muscle cells in culture

AU - Nakao, Junko

AU - Chang, Wen Chang

AU - Murota, Sei Itsu

AU - Orimo, Hajime

N1 - Funding Information: The authors wish to thank Mrs. Y. Kotera for her highly skillful technical assistance and Ono Pharmaceutical Co. Ltd., Oosaka, Japan, for the generous gifts of standard prostaglandins and thromboxane Bz and the financial support for W.C. Chang. Thanks are also due to Dr. K.Yu. of the Yale University School of Medicine, New Haven, CN, U.S.A., for reading the manuscript.

PY - 1981/7

Y1 - 1981/7

N2 - The effect of triiodothyronine (T3) on prostacyclin production in rat aortic smooth muscle cells was investigated by using an intact cell assay system. T3 at its physiological concentrations has no significant effect on smooth muscle cell proliferation, but it does significantly stimulate prostacyclin production by the cells. Maximum stimulation of prostacyclin production is obtained when cells are treated with T3 for 4 consecutive days. The dose-response curve shows a linear relationship between the stimulation of prostacyclin production and T3 concentrations in the range 0.007-10 μg/dl. The maximal prostacyclin production by T3-treated cells (at a concentration of 10 μg/dl), is 270% compared with control cells. T3 treatment shows no significant effect on phospholipase (and/or lipase) activities. Our results suggest that thyroid hormone might play an important physiological role in the protection of arteries from atherosclerotic changes by stimulating prostacyclin production in arterial smooth muscle cells.

AB - The effect of triiodothyronine (T3) on prostacyclin production in rat aortic smooth muscle cells was investigated by using an intact cell assay system. T3 at its physiological concentrations has no significant effect on smooth muscle cell proliferation, but it does significantly stimulate prostacyclin production by the cells. Maximum stimulation of prostacyclin production is obtained when cells are treated with T3 for 4 consecutive days. The dose-response curve shows a linear relationship between the stimulation of prostacyclin production and T3 concentrations in the range 0.007-10 μg/dl. The maximal prostacyclin production by T3-treated cells (at a concentration of 10 μg/dl), is 270% compared with control cells. T3 treatment shows no significant effect on phospholipase (and/or lipase) activities. Our results suggest that thyroid hormone might play an important physiological role in the protection of arteries from atherosclerotic changes by stimulating prostacyclin production in arterial smooth muscle cells.

KW - Aortic smooth muscle cells

KW - Atherosclerosis

KW - Prostacyclin

KW - Triiodothyronine

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ER -

Triiodothyronine stimulates prostacyclin production by rat aortic smooth muscle cells in culture

Abstract

Keywords

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