Treadmill running upregulates the expression of acetylcholine receptor in rat gastrocnemius following botulinum toxin A injection

Sen Wei Tsai, Yu Tang Tung, Hsiao Ling Chen, Chih Jie Shen, Chia Hui Chuang, Ting Yu Tang, Chuan Mu Chen

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)


Treadmill running is a commonly used training method for patients with spasticity to improve functional performance. Botulinum toxin has been widely used therapeutically to reduce contraction force of spastic muscle. However, the effects of treadmill running in neuromuscular junction expression and motor unit physiology on muscle following botulinum toxin injection are not well established. To assess the effects of treadmill running on neuromuscular recovery of gastrocnemius following botulinum toxin A (BoNT-A) injection, we observed changes in gene expression. We hypothesized that the expression of acetylcholine receptor (AChR), myogenesis, and nerve plasticity could be enhanced. Twenty-four Sprague-Dawley rats received botulinum toxin injection in right gastrocnemius and were then randomly assigned into untrained control and treadmill running groups. The rats assigned to the treadmill running group were trained on a treadmill 3 times/week with a running speed of 15 m/min for 8 weeks. The duration of training was 20 min per session. Muscle strength and gene expression of AChR subunit (α, β, Î, Î, and Îμ), MyoD, Myf-5, MRF4, myogenin, p21, IGF-1, GAP43, were analyzed. Treadmill running had no influence on gastrocnemius mass, but improved the maximal contraction force of the gastrocnemius in the treadmill running group (p < 0.05). Upregulation of GAP-43, IGF-1, Myo-D, Myf-5, myogenin, and AChR subunits α and β were found following treadmill running. The expression of genes associated with neurite and AChR regeneration following treadmill exercise was upregulated, which may have contributed to enhanced recovery of gastrocnemius strength.

Original languageEnglish
Pages (from-to)125-131
Number of pages7
JournalJournal of Orthopaedic Research
Issue number1
Publication statusPublished - Jan 2013
Externally publishedYes


  • acetylcholine receptor
  • botulinum toxin
  • neuromuscular junction
  • treadmill

ASJC Scopus subject areas

  • Orthopedics and Sports Medicine


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