TY - JOUR
T1 - Transient expression analysis of the reticuloendotheliosis virus long terminal repeat element
AU - Ridgway, Anthony A.G.
AU - Kung, Hsing Jien
AU - Fujita, Donald J.
N1 - Funding Information:
This work was supported initially by a grant to D. F. and continued under a grant to A.R., both from the National Cancer Institute of Canada.
Copyright:
Copyright 2010 Elsevier B.V., All rights reserved.
PY - 1989/4/25
Y1 - 1989/4/25
N2 - A region of the Reticuloendotheliosis virus (REV) long terminal repeat (LTR) harbouring single or duplicated copies of 46-bp and 26-bp sequence elements is implicated in enhancer activity. Sequences residing upstream from the proviral 3′ LTR did not contribute to activity of the intact LTR. Gene expression regulated by a combination of REV enhancer and SV40 early region promoter was 50-fold less than from the analogous construct containing the chicken syncytial virus promoter. Deletion of LTR sequences immediately downstream of the CAP site, which include a region capable of forming a stable hairpin in the mRNA, decreased expression by 70%. Expression assays and S1 nuclease mapping showed that a second transcriptional start site, identified by transcription in vitro using HeLa cell lysates and purified DNA templates, was not used in vivo in the cell lines examined.
AB - A region of the Reticuloendotheliosis virus (REV) long terminal repeat (LTR) harbouring single or duplicated copies of 46-bp and 26-bp sequence elements is implicated in enhancer activity. Sequences residing upstream from the proviral 3′ LTR did not contribute to activity of the intact LTR. Gene expression regulated by a combination of REV enhancer and SV40 early region promoter was 50-fold less than from the analogous construct containing the chicken syncytial virus promoter. Deletion of LTR sequences immediately downstream of the CAP site, which include a region capable of forming a stable hairpin in the mRNA, decreased expression by 70%. Expression assays and S1 nuclease mapping showed that a second transcriptional start site, identified by transcription in vitro using HeLa cell lysates and purified DNA templates, was not used in vivo in the cell lines examined.
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U2 - 10.1093/nar/17.8.3199
DO - 10.1093/nar/17.8.3199
M3 - Article
C2 - 2542893
AN - SCOPUS:0024520012
SN - 0305-1048
VL - 17
SP - 3199
EP - 3215
JO - Nucleic Acids Research
JF - Nucleic Acids Research
IS - 8
ER -