Transgenic expression of tilapia piscidin 3 (TP3) in zebrafish confers resistance to Streptococcus agalactiae

Bor Chyuan Su, Yung Wei Lai, Jyh Yih Chen, Chieh Yu Pan

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)


To study the biological role of tilapia piscidin 3 (TP3) in Streptococcus agalactiae infection in vivo, TP3/DsRed overexpressing transgenic zebrafish were generated. Under normal growth conditions, TP3/DsRed transgenic zebrafish exhibited an orange-red body color, without any other obvious abnormalities. However, when compared to wild type fish, TP3/DsRed transgenic zebrafish were resistant to S. agalactiae infection. After infection, the TP3 overexpressing fish exhibited higher expression of Toll-like receptor 4a (TLR4a), interleukin (IL)-10, IL-22, and C3b. Furthermore, TP3/DsRed transgenic zebrafish exhibited reduced induction of proinflammatory cytokines, including TNFα IL-1β IL-21, MyD88, and nuclear factor (NF)-κB. Taken together, our data show that TP3 overexpression in zebrafish can effectively suppress proinflammatory responses and enhance production of C3b. Together, these actions are conducive to the resolution of inflammation and bacterial clearance. We further postulate that TP3 may exert its anti-inflammatory effects by enhancing TLR4a-mediated negative regulation of NF-κB.

Original languageEnglish
Pages (from-to)235-241
Number of pages7
JournalFish and Shellfish Immunology
Publication statusPublished - Mar 1 2018
Externally publishedYes


  • Streptococcus agalactiae
  • Tilapia piscidin 3
  • Zebrafish

ASJC Scopus subject areas

  • Environmental Chemistry
  • Aquatic Science


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