TY - JOUR
T1 - Transforming growth factor β1 down-regulates Runx-2 and alkaline phosphatase activity of human dental pulp cells via ALK5/Smad2/3 signaling
AU - Lin, Po Shuen
AU - Chang, Mei Chi
AU - Chan, Chiu Po
AU - Lee, Sheng Yang
AU - Lee, Jang Jaer
AU - Tsai, Yi Ling
AU - Tseng, Hui Chun
AU - Tai, Tseng Fang
AU - Lin, Hsueh Jen
AU - Jeng, Jiiang Huei
PY - 2011/3
Y1 - 2011/3
N2 - Objective Transforming growth factor β1 (TGF-β1) plays a role in repair and dentinogenesis in dental pulp. The purpose of this study was to study how TGF-β1 affects 2 differentiation markers, Runt-related transcription factor 2 (Runx-2) and ALP, in dental pulp cells. Study design Primary-cultured human dental pulp cells were treated with TGF-β1 with or without pretreatment and coincubation with 1,4-diamino-2,3-dicyano-1,4-bis(o- aminophenylmercapto)butadiene (U0126, a mitogen-induced extracellular kinase (MEK)/extracellular signal-regulated kinase (ERK) inhibitor), Noggin (a bone morphogenetic protein inhibitor), or 4-(5-benzol[1,3]dioxol-5-yl-4-pyrldin-2-yl- 1H-imidazol-2-yl)-benzamide hydrate (SB431542, an activin receptorlike kinase (ALK) 5/Smad2/3 inhibitor). The differentiation status of pulp cells was evaluated by ALP staining and quantitative ALP activity assay. Changes in ALP and Runx-2 mRNA expression were determined by reverse-transcription polymerase chain reaction. Results Cells under the treatment of TGF-β1 (5 and 10 ng/mL) showed a decrease in ALP activity and gene expression of ALP and Runx-2. Pretreatment by U0126 and Noggin was not effective to prevent the TGF-β1induced decline of ALP activity. Interestingly, SB431542 prevented the TGF-β1induced decline of ALP activity and ALP and Runx-2 gene expression. Conclusion TGF-β1 down-regulates Runx-2 and ALP in human dental pulp cells via ALK5/Smad2/3 signaling. These events may play important roles at specific stages of pulpal repair and dentinogenesis.
AB - Objective Transforming growth factor β1 (TGF-β1) plays a role in repair and dentinogenesis in dental pulp. The purpose of this study was to study how TGF-β1 affects 2 differentiation markers, Runt-related transcription factor 2 (Runx-2) and ALP, in dental pulp cells. Study design Primary-cultured human dental pulp cells were treated with TGF-β1 with or without pretreatment and coincubation with 1,4-diamino-2,3-dicyano-1,4-bis(o- aminophenylmercapto)butadiene (U0126, a mitogen-induced extracellular kinase (MEK)/extracellular signal-regulated kinase (ERK) inhibitor), Noggin (a bone morphogenetic protein inhibitor), or 4-(5-benzol[1,3]dioxol-5-yl-4-pyrldin-2-yl- 1H-imidazol-2-yl)-benzamide hydrate (SB431542, an activin receptorlike kinase (ALK) 5/Smad2/3 inhibitor). The differentiation status of pulp cells was evaluated by ALP staining and quantitative ALP activity assay. Changes in ALP and Runx-2 mRNA expression were determined by reverse-transcription polymerase chain reaction. Results Cells under the treatment of TGF-β1 (5 and 10 ng/mL) showed a decrease in ALP activity and gene expression of ALP and Runx-2. Pretreatment by U0126 and Noggin was not effective to prevent the TGF-β1induced decline of ALP activity. Interestingly, SB431542 prevented the TGF-β1induced decline of ALP activity and ALP and Runx-2 gene expression. Conclusion TGF-β1 down-regulates Runx-2 and ALP in human dental pulp cells via ALK5/Smad2/3 signaling. These events may play important roles at specific stages of pulpal repair and dentinogenesis.
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U2 - 10.1016/j.tripleo.2010.09.079
DO - 10.1016/j.tripleo.2010.09.079
M3 - Article
C2 - 21236710
AN - SCOPUS:79951499634
SN - 1079-2104
VL - 111
SP - 394
EP - 400
JO - Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics
JF - Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics
IS - 3
ER -