TY - JOUR
T1 - Transcranial photobiomodulation attenuates pentylenetetrazole-induced status epilepticus in peripubertal rats
AU - Tsai, Chung Min
AU - Chang, Shwu Fen
AU - Chang, Hsi
N1 - Funding Information:
The authors acknowledge professor Geng-Chang Yeh for his visionary innovation, initiation and guidance in the application of tPBM to rats with PTZ-induced SE and in the strategies for the histobiochemical studies. We thank Yu-Han Tsao for technical assistance and for assisting with a portion of the transcardial perfusion and brain harvest procedures. We thank Anthony Abram (www.uni-edit.net) for editing and proofreading this manuscript.
Publisher Copyright:
© 2020 The Authors. Journal of Biophotonics published by WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.
PY - 2020/8/1
Y1 - 2020/8/1
N2 - Convulsive status epilepticus is the most common neurological emergency in children. Transcranial photobiomodulation (tPBM) reverses elevated rodent neurotransmitters after status epilepticus (SE) yet whether tPBM can attenuate seizure behaviors remains unknown. Here, we applied near-infrared laser at wavelength 808 nm transcranially to peripubertal Sprague-Dawley rats prior to pentylenetetrazole (PTZ) injection. Hematoxylin-eosin, immunofluorescence (IF) staining with anti-parvalbumin (PV) and terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) assay after IF staining was performed. Behaviorally, tPBM attenuated the mean seizure score and reduced the incidence of SE and mortality. Histochemically, tPBM reduced dark neurons in the cortex, hippocampus, thalamus and hypothalamus, lessened the apoptotic ratio of parvalbumin-positive interneurons (PV-INs) and alleviated the aberrant extent of PV-positive unstained somata of PCs in the hippocampus. Conclusively, tPBM attenuated PTZ-induced seizures, SE and mortality in peripubertal rats and reduced PTZ-induced neuronal injury, apoptosis of PV-INs and preserved PV positive perisomatic inhibitory network in the hippocampus.
AB - Convulsive status epilepticus is the most common neurological emergency in children. Transcranial photobiomodulation (tPBM) reverses elevated rodent neurotransmitters after status epilepticus (SE) yet whether tPBM can attenuate seizure behaviors remains unknown. Here, we applied near-infrared laser at wavelength 808 nm transcranially to peripubertal Sprague-Dawley rats prior to pentylenetetrazole (PTZ) injection. Hematoxylin-eosin, immunofluorescence (IF) staining with anti-parvalbumin (PV) and terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) assay after IF staining was performed. Behaviorally, tPBM attenuated the mean seizure score and reduced the incidence of SE and mortality. Histochemically, tPBM reduced dark neurons in the cortex, hippocampus, thalamus and hypothalamus, lessened the apoptotic ratio of parvalbumin-positive interneurons (PV-INs) and alleviated the aberrant extent of PV-positive unstained somata of PCs in the hippocampus. Conclusively, tPBM attenuated PTZ-induced seizures, SE and mortality in peripubertal rats and reduced PTZ-induced neuronal injury, apoptosis of PV-INs and preserved PV positive perisomatic inhibitory network in the hippocampus.
KW - apoptosis
KW - hippocampus
KW - parvalbumin-positive interneurons
KW - PTZ
KW - seizures
KW - transcranial photobiomodulation
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U2 - 10.1002/jbio.202000095
DO - 10.1002/jbio.202000095
M3 - Article
C2 - 32362066
AN - SCOPUS:85084814903
SN - 1864-063X
VL - 13
JO - Journal of Biophotonics
JF - Journal of Biophotonics
IS - 8
M1 - e202000095
ER -