Toxicity assessments of Chalcone and some Synthetic Chalcone Analogues in a Zebrafish model

Ya Ting Lee; Tsorng Harn Fong; Hui Min Chen; Chao Yuan Chang; Yun Hsin Wang; Ching Yuh Chern; Yau Hung Chen

doi:10.3390/molecules19010641

Toxicity assessments of Chalcone and some Synthetic Chalcone Analogues in a Zebrafish model

Ya Ting Lee, Tsorng Harn Fong, Hui Min Chen, Chao Yuan Chang, Yun Hsin Wang, Ching Yuh Chern, Yau Hung Chen

Department of Anatomy and Cell Biology

Research output: Contribution to journal › Article › peer-review

24 Citations (Scopus)

Abstract

The aim of this study was to investigate the in vivo toxicities of some novel synthetic chalcones. Chalcone and four chalcone analogues 1a-d were evaluated using zebrafish embryos following antibody staining to visualize their morphological changes and muscle fiber alignment. Results showed that embryos treated with 3'-hydroxychalcone (compound 1b) displayed a high percentage of muscle defects (96.6%), especially myofibril misalignment. Ultrastructural analysis revealed that compound 1b-treated embryos displayed many muscle defect phenotypes, including breakage and collapse of myofibrils, reduced cell numbers, and disorganized thick (myosin) and thin (actin) filaments. Taken together, our results provide in vivo evidence of the myotoxic effects of the synthesized chalcone analogues on developing zebrafish embryos.

Original language	English
Pages (from-to)	641-650
Number of pages	10
Journal	Molecules
Volume	19
Issue number	1
DOIs	https://doi.org/10.3390/molecules19010641
Publication status	Published - Jan 2014

Keywords

Chalcone
Embryogenesis
Muscle
Toxicity
Zebrafish

ASJC Scopus subject areas

Drug Discovery
Analytical Chemistry
Chemistry (miscellaneous)
Molecular Medicine
Physical and Theoretical Chemistry
Pharmaceutical Science
Organic Chemistry

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10.3390/molecules19010641

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title = "Toxicity assessments of Chalcone and some Synthetic Chalcone Analogues in a Zebrafish model",

abstract = "The aim of this study was to investigate the in vivo toxicities of some novel synthetic chalcones. Chalcone and four chalcone analogues 1a-d were evaluated using zebrafish embryos following antibody staining to visualize their morphological changes and muscle fiber alignment. Results showed that embryos treated with 3'-hydroxychalcone (compound 1b) displayed a high percentage of muscle defects (96.6%), especially myofibril misalignment. Ultrastructural analysis revealed that compound 1b-treated embryos displayed many muscle defect phenotypes, including breakage and collapse of myofibrils, reduced cell numbers, and disorganized thick (myosin) and thin (actin) filaments. Taken together, our results provide in vivo evidence of the myotoxic effects of the synthesized chalcone analogues on developing zebrafish embryos.",

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author = "Lee, {Ya Ting} and Fong, {Tsorng Harn} and Chen, {Hui Min} and Chang, {Chao Yuan} and Wang, {Yun Hsin} and Chern, {Ching Yuh} and Chen, {Yau Hung}",

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AU - Lee, Ya Ting

AU - Fong, Tsorng Harn

AU - Chen, Hui Min

AU - Chang, Chao Yuan

AU - Wang, Yun Hsin

AU - Chern, Ching Yuh

AU - Chen, Yau Hung

PY - 2014/1

Y1 - 2014/1

N2 - The aim of this study was to investigate the in vivo toxicities of some novel synthetic chalcones. Chalcone and four chalcone analogues 1a-d were evaluated using zebrafish embryos following antibody staining to visualize their morphological changes and muscle fiber alignment. Results showed that embryos treated with 3'-hydroxychalcone (compound 1b) displayed a high percentage of muscle defects (96.6%), especially myofibril misalignment. Ultrastructural analysis revealed that compound 1b-treated embryos displayed many muscle defect phenotypes, including breakage and collapse of myofibrils, reduced cell numbers, and disorganized thick (myosin) and thin (actin) filaments. Taken together, our results provide in vivo evidence of the myotoxic effects of the synthesized chalcone analogues on developing zebrafish embryos.

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KW - Muscle

KW - Toxicity

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Toxicity assessments of Chalcone and some Synthetic Chalcone Analogues in a Zebrafish model

Abstract

Keywords

ASJC Scopus subject areas

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