Topoisomerase i inhibitor evodiamine acts as an antibacterial agent against drug-resistant Klebsiella pneumoniae

Jui Yu Wu; Ming Chih Chang; Chien Shu Chen; Hsiu Chen Lin; Hsiang Ping Tsai; Chien Chun Yang; Che Hsiung Yang; Chun Mao Lin

doi:10.1055/s-0032-1327925

Topoisomerase i inhibitor evodiamine acts as an antibacterial agent against drug-resistant Klebsiella pneumoniae

Jui Yu Wu, Ming Chih Chang, Chien Shu Chen, Hsiu Chen Lin, Hsiang Ping Tsai, Chien Chun Yang, Che Hsiung Yang, Chun Mao Lin

Research output: Contribution to journal › Article › peer-review

22 Citations (Scopus)

Abstract

Topoisomerase inhibitors have been developed in a variety of clinical applications. We investigated the inhibitory effect of evodiamine on E. coli topoisomerase I, which may lead to an anti-bacterial effect. Evodiamine inhibits the supercoiled plasmid DNA relaxation that is catalyzed by E. coli topoisomerase I, and computer-aided docking has shown that the Arg161 and Asp551 residues of topoisomerase I interact with evodiamine. We investigated the bactericidal effect of evodiamine against multidrug-resistant Klebsiella pneumoniae. Evodiamine showed a significantly lower minimal inhibitory concentration value (MIC 128 μg/mL) compared with antibiotics (> 512 μg/mL) against the clinical isolate of K. pneumoniae. The results suggested that evodiamine is a potential agent against drug-resistant bacteria.

Original language	English
Pages (from-to)	27-29
Number of pages	3
Journal	Planta Medica
Volume	79
Issue number	1
DOIs	https://doi.org/10.1055/s-0032-1327925
Publication status	Published - 2013

Keywords

K. pneumonia
antibiotic
drug resistance
evodiamine
zopoisomerase

ASJC Scopus subject areas

Drug Discovery
Analytical Chemistry
Molecular Medicine
Complementary and alternative medicine
Pharmacology
Pharmaceutical Science
Organic Chemistry

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10.1055/s-0032-1327925

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title = "Topoisomerase i inhibitor evodiamine acts as an antibacterial agent against drug-resistant Klebsiella pneumoniae",

abstract = "Topoisomerase inhibitors have been developed in a variety of clinical applications. We investigated the inhibitory effect of evodiamine on E. coli topoisomerase I, which may lead to an anti-bacterial effect. Evodiamine inhibits the supercoiled plasmid DNA relaxation that is catalyzed by E. coli topoisomerase I, and computer-aided docking has shown that the Arg161 and Asp551 residues of topoisomerase I interact with evodiamine. We investigated the bactericidal effect of evodiamine against multidrug-resistant Klebsiella pneumoniae. Evodiamine showed a significantly lower minimal inhibitory concentration value (MIC 128 μg/mL) compared with antibiotics (> 512 μg/mL) against the clinical isolate of K. pneumoniae. The results suggested that evodiamine is a potential agent against drug-resistant bacteria.",

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T1 - Topoisomerase i inhibitor evodiamine acts as an antibacterial agent against drug-resistant Klebsiella pneumoniae

AU - Wu, Jui Yu

AU - Chang, Ming Chih

AU - Chen, Chien Shu

AU - Lin, Hsiu Chen

AU - Tsai, Hsiang Ping

AU - Yang, Chien Chun

AU - Yang, Che Hsiung

AU - Lin, Chun Mao

PY - 2013

Y1 - 2013

N2 - Topoisomerase inhibitors have been developed in a variety of clinical applications. We investigated the inhibitory effect of evodiamine on E. coli topoisomerase I, which may lead to an anti-bacterial effect. Evodiamine inhibits the supercoiled plasmid DNA relaxation that is catalyzed by E. coli topoisomerase I, and computer-aided docking has shown that the Arg161 and Asp551 residues of topoisomerase I interact with evodiamine. We investigated the bactericidal effect of evodiamine against multidrug-resistant Klebsiella pneumoniae. Evodiamine showed a significantly lower minimal inhibitory concentration value (MIC 128 μg/mL) compared with antibiotics (> 512 μg/mL) against the clinical isolate of K. pneumoniae. The results suggested that evodiamine is a potential agent against drug-resistant bacteria.

AB - Topoisomerase inhibitors have been developed in a variety of clinical applications. We investigated the inhibitory effect of evodiamine on E. coli topoisomerase I, which may lead to an anti-bacterial effect. Evodiamine inhibits the supercoiled plasmid DNA relaxation that is catalyzed by E. coli topoisomerase I, and computer-aided docking has shown that the Arg161 and Asp551 residues of topoisomerase I interact with evodiamine. We investigated the bactericidal effect of evodiamine against multidrug-resistant Klebsiella pneumoniae. Evodiamine showed a significantly lower minimal inhibitory concentration value (MIC 128 μg/mL) compared with antibiotics (> 512 μg/mL) against the clinical isolate of K. pneumoniae. The results suggested that evodiamine is a potential agent against drug-resistant bacteria.

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KW - antibiotic

KW - drug resistance

KW - evodiamine

KW - zopoisomerase

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Topoisomerase i inhibitor evodiamine acts as an antibacterial agent against drug-resistant Klebsiella pneumoniae

Abstract

Keywords

ASJC Scopus subject areas

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