Titanium implants alter endothelial function and vasoconstriction via a protein kinase C-regulated pathway

Rong Sen Yang; Huei Ping Tzeng; Feng Ming Ho; Chia Chi Chuang; Bo Lin Chen; Chun Fa Huang; Ya Wen Chen; Ruei Ming Chen; Shing Hwa Liu

doi:10.1016/j.actbio.2009.05.006

Titanium implants alter endothelial function and vasoconstriction via a protein kinase C-regulated pathway

Rong Sen Yang, Huei Ping Tzeng, Feng Ming Ho, Chia Chi Chuang, Bo Lin Chen, Chun Fa Huang, Ya Wen Chen, Ruei Ming Chen, Shing Hwa Liu

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

The application of titanium (Ti) alloy in joint prostheses is a good choice in orthopedic reconstruction. An elevated serum concentration of Ti has been shown in the patients with loosened knee prostheses. The precise actions of elevated Ti on the circulation remain unclear. In this study the maximal contractile responses elicited by phenylephrine in the aortas of rats 4 weeks after Ti alloy implantation and in cultured rat aortas treated with a soluble form of Ti for a period of 18 h were significantly decreased as compared with controls. Aortas isolated from rats with Ti alloy implants or aortas treated with a soluble form of Ti had enhanced protein expression of endothelial nitric oxide synthase (eNOS) and protein kinase C (PKC)-α and enhanced phosphorylation of extracellular signal-regulated kinase (ERK) 1/2. Treatment of human umbilical vein endothelial cells (HUVECs) with a soluble form of Ti for 24 h dose-dependently increased eNOS protein expression. Short-term treatment of HUVECs with Ti for 1 h effectively enhanced the phosphorylation of eNOS, PKC (pan) and ERK1/2. PKC inhibitors RO320432 and chelerythrine effectively inhibited Ti-enhanced phosphorylation of eNOS and PKC (pan). These results indicate that Ti in the circulation may alter endothelial function and reduce vasoconstriction.

Original language	English
Pages (from-to)	3258-3264
Number of pages	7
Journal	Acta Biomaterialia
Volume	5
Issue number	8
DOIs	https://doi.org/10.1016/j.actbio.2009.05.006
Publication status	Published - Oct 2009

Keywords

Aorta
Endothelial cells
Titanium alloy implants
eNOS

ASJC Scopus subject areas

Biotechnology
Biomaterials
Biochemistry
Biomedical Engineering
Molecular Biology

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10.1016/j.actbio.2009.05.006

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title = "Titanium implants alter endothelial function and vasoconstriction via a protein kinase C-regulated pathway",

abstract = "The application of titanium (Ti) alloy in joint prostheses is a good choice in orthopedic reconstruction. An elevated serum concentration of Ti has been shown in the patients with loosened knee prostheses. The precise actions of elevated Ti on the circulation remain unclear. In this study the maximal contractile responses elicited by phenylephrine in the aortas of rats 4 weeks after Ti alloy implantation and in cultured rat aortas treated with a soluble form of Ti for a period of 18 h were significantly decreased as compared with controls. Aortas isolated from rats with Ti alloy implants or aortas treated with a soluble form of Ti had enhanced protein expression of endothelial nitric oxide synthase (eNOS) and protein kinase C (PKC)-α and enhanced phosphorylation of extracellular signal-regulated kinase (ERK) 1/2. Treatment of human umbilical vein endothelial cells (HUVECs) with a soluble form of Ti for 24 h dose-dependently increased eNOS protein expression. Short-term treatment of HUVECs with Ti for 1 h effectively enhanced the phosphorylation of eNOS, PKC (pan) and ERK1/2. PKC inhibitors RO320432 and chelerythrine effectively inhibited Ti-enhanced phosphorylation of eNOS and PKC (pan). These results indicate that Ti in the circulation may alter endothelial function and reduce vasoconstriction.",

keywords = "Aorta, Endothelial cells, Titanium alloy implants, eNOS",

author = "Yang, {Rong Sen} and Tzeng, {Huei Ping} and Ho, {Feng Ming} and Chuang, {Chia Chi} and Chen, {Bo Lin} and Huang, {Chun Fa} and Chen, {Ya Wen} and Chen, {Ruei Ming} and Liu, {Shing Hwa}",

note = "Funding Information: This study was supported by a grant from the National Science Council of Taiwan (NSC92-2314-B-002-094).",

year = "2009",

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doi = "10.1016/j.actbio.2009.05.006",

language = "English",

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T1 - Titanium implants alter endothelial function and vasoconstriction via a protein kinase C-regulated pathway

AU - Yang, Rong Sen

AU - Tzeng, Huei Ping

AU - Ho, Feng Ming

AU - Chuang, Chia Chi

AU - Chen, Bo Lin

AU - Huang, Chun Fa

AU - Chen, Ya Wen

AU - Chen, Ruei Ming

AU - Liu, Shing Hwa

N1 - Funding Information: This study was supported by a grant from the National Science Council of Taiwan (NSC92-2314-B-002-094).

PY - 2009/10

Y1 - 2009/10

N2 - The application of titanium (Ti) alloy in joint prostheses is a good choice in orthopedic reconstruction. An elevated serum concentration of Ti has been shown in the patients with loosened knee prostheses. The precise actions of elevated Ti on the circulation remain unclear. In this study the maximal contractile responses elicited by phenylephrine in the aortas of rats 4 weeks after Ti alloy implantation and in cultured rat aortas treated with a soluble form of Ti for a period of 18 h were significantly decreased as compared with controls. Aortas isolated from rats with Ti alloy implants or aortas treated with a soluble form of Ti had enhanced protein expression of endothelial nitric oxide synthase (eNOS) and protein kinase C (PKC)-α and enhanced phosphorylation of extracellular signal-regulated kinase (ERK) 1/2. Treatment of human umbilical vein endothelial cells (HUVECs) with a soluble form of Ti for 24 h dose-dependently increased eNOS protein expression. Short-term treatment of HUVECs with Ti for 1 h effectively enhanced the phosphorylation of eNOS, PKC (pan) and ERK1/2. PKC inhibitors RO320432 and chelerythrine effectively inhibited Ti-enhanced phosphorylation of eNOS and PKC (pan). These results indicate that Ti in the circulation may alter endothelial function and reduce vasoconstriction.

AB - The application of titanium (Ti) alloy in joint prostheses is a good choice in orthopedic reconstruction. An elevated serum concentration of Ti has been shown in the patients with loosened knee prostheses. The precise actions of elevated Ti on the circulation remain unclear. In this study the maximal contractile responses elicited by phenylephrine in the aortas of rats 4 weeks after Ti alloy implantation and in cultured rat aortas treated with a soluble form of Ti for a period of 18 h were significantly decreased as compared with controls. Aortas isolated from rats with Ti alloy implants or aortas treated with a soluble form of Ti had enhanced protein expression of endothelial nitric oxide synthase (eNOS) and protein kinase C (PKC)-α and enhanced phosphorylation of extracellular signal-regulated kinase (ERK) 1/2. Treatment of human umbilical vein endothelial cells (HUVECs) with a soluble form of Ti for 24 h dose-dependently increased eNOS protein expression. Short-term treatment of HUVECs with Ti for 1 h effectively enhanced the phosphorylation of eNOS, PKC (pan) and ERK1/2. PKC inhibitors RO320432 and chelerythrine effectively inhibited Ti-enhanced phosphorylation of eNOS and PKC (pan). These results indicate that Ti in the circulation may alter endothelial function and reduce vasoconstriction.

KW - Aorta

KW - Endothelial cells

KW - Titanium alloy implants

KW - eNOS

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Titanium implants alter endothelial function and vasoconstriction via a protein kinase C-regulated pathway

Abstract

Keywords

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