Thyroid hormone-induced expression of inflammatory cytokines interfere with resveratrol-induced anti-proliferation of oral cancer cells

Yi Ru Chen, Yu Shen Chen, Yu Tang Chin, Zi Lin Li, Ya Jung Shih, Yu Chen S.H. Yang, Chun A. ChangOu, Po Yu Su, Shwu Huey Wang, Yun Hsuan Wu, Hsien Chung Chiu, Sheng Yang Lee, Leroy F. Liu, Jacqueline Whang-Peng, Hung Yun Lin, Shaker A. Mousa, Paul J. Davis, Kuan Wang

Research output: Contribution to journalArticlepeer-review

22 Citations (Scopus)


Thyroid hormone, L-thyroxine (T4), induces inflammatory genes expressions and promotes cancer growth. It also induces expression of the checkpoint programmed death-ligand 1 (PD-L1), which plays a vital role in cancer progression. On the other hand, resveratrol inhibits inflammatory genes expressions. Moreover, resveratrol increases nuclear inducible cyclooxygenase (COX)-2 accumulation, complexes with p53, and induces p53-dependent anti-proliferation. In this study, we investigated the effect of T4 on resveratrol-induced anti-proliferation in oral cancer. T4 increased the expression and cytoplasmic accumulation of PD-L1. Increased expressions of pro-inflammatory genes, interleukin (IL)-1β and transforming growth factor (TGF)-β1, were shown to stimulate PD-L1 expression. T4 stimulated pro-inflammatory and proliferative genes expressions, and oral cancer cells proliferation. In contrast, resveratrol inhibited those genes and activated anti-proliferative genes. T4 retained resveratrol-induced COX-2 in cytoplasm and prevented COX-2 nuclear accumulation when resveratrol treated cancer cells. A specific signal transducer and activator of transcription 3 (STAT3) inhibitor, S31-201, blocked T4-induced inhibition and restored resveratrol-induced nuclear COX-2 accumulation. By inhibiting the T4-activated STAT3 signal transduction axis with S31-201, resveratrol was able to sequentially reestablish COX-2/p53-dependent gene expressions and anti-proliferation. These findings provide a novel understanding of the inhibitory effects of T4 on resveratrol-induced anticancer properties via the sequential expression of PD-L1 and inflammatory genes.

Original languageEnglish
Article number110693
JournalFood and Chemical Toxicology
Publication statusPublished - Oct 2019


  • Inflammation
  • L-thyroxine
  • Oral cancer
  • PD-L1
  • Resveratrol

ASJC Scopus subject areas

  • Food Science
  • Toxicology


Dive into the research topics of 'Thyroid hormone-induced expression of inflammatory cytokines interfere with resveratrol-induced anti-proliferation of oral cancer cells'. Together they form a unique fingerprint.

Cite this