Three dimensional microfluidics with embedded microball lenses for parallel and high throughput multicolor fluorescence detection

Y. J. Fan, Y. C. Wu, Y. Chen, Y. C. Kung, T. H. Wu, K. W. Huang, H. J. Sheen, P. Y. Chiou

Research output: Contribution to journalArticlepeer-review

32 Citations (Scopus)


We report a 3D microfluidic device with 32 detection channels and 64 sheath flow channels and embedded microball lens array for high throughput multicolor fluorescence detection. A throughput of 358?400 cells/s has been accomplished. This device is realized by utilizing solid immersion micro ball lens arrays for high sensitivity and parallel fluorescence detection. High refractive index micro ball lenses (n?=?2.1) are embedded underneath PDMS channels close to cell detection zones in channels. This design permits patterning high N.A. micro ball lenses in a compact fashion for parallel fluorescence detection on a small footprint device. This device also utilizes 3D microfluidic fabrication to address fluid routing issues in two-dimensional parallel sheath focusing and allows simultaneous pumping of 32 sample channels and 64 sheath flow channels with only two inlets.
Original languageChinese (Traditional)
Issue number4
Publication statusPublished - Jul 1 2013
Externally publishedYes

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