The variant mRNA isoform of human metastasis gene (CD44V) detected in the cell lines of human hepatocellular carcinoma

H. J. Harn; L. I. Ho; C. P. Yu; M. W. Wang; H. S. Lee; J. J. Lin; W. H. Lee; N. R. Isola; D. L. Cooper

The variant mRNA isoform of human metastasis gene (CD44V) detected in the cell lines of human hepatocellular carcinoma

H. J. Harn, L. I. Ho, C. P. Yu, M. W. Wang, H. S. Lee, J. J. Lin, W. H. Lee, N. R. Isola, D. L. Cooper

Research output: Contribution to journal › Article › peer-review

Abstract

The interaction of the cell surface receptor CD44 molecular with its ligands (addressin, extracellular matrix etc.,) plays an important role in fulfilling the lymphocyte homing and immune reaction. Recently alternatively spliced products of CD44 gene are found to be involved in tumor metastasis as well. Our report found that CD44 prototype RNA (CD44S) was present in all five tumor cell lines. Isoform CD44 RNA (CD44V) was recognized in three metastasized hepatocellular carcinoma cell lines, J5, HCC36, HEP38. In addition, the J5 CD44 RNA isoform expressed two distinct trancripts which are of the same size as MDA-231 breast tumor cell line. The MDA-231 CD44 RNA variant (CD44V) has been confirmed to contain metastasis domain 4 and 5. it is implicated that the alternative RNA splicing may also play a major role in hepatocellular carcinoma metastasis.

Original language	English
Pages (from-to)	233-238
Number of pages	6
Journal	Biochemistry and Molecular Biology International
Volume	32
Issue number	2
Publication status	Published - 1994
Externally published	Yes

ASJC Scopus subject areas

Genetics
Molecular Biology
Biochemistry

Cite this

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title = "The variant mRNA isoform of human metastasis gene (CD44V) detected in the cell lines of human hepatocellular carcinoma",

abstract = "The interaction of the cell surface receptor CD44 molecular with its ligands (addressin, extracellular matrix etc.,) plays an important role in fulfilling the lymphocyte homing and immune reaction. Recently alternatively spliced products of CD44 gene are found to be involved in tumor metastasis as well. Our report found that CD44 prototype RNA (CD44S) was present in all five tumor cell lines. Isoform CD44 RNA (CD44V) was recognized in three metastasized hepatocellular carcinoma cell lines, J5, HCC36, HEP38. In addition, the J5 CD44 RNA isoform expressed two distinct trancripts which are of the same size as MDA-231 breast tumor cell line. The MDA-231 CD44 RNA variant (CD44V) has been confirmed to contain metastasis domain 4 and 5. it is implicated that the alternative RNA splicing may also play a major role in hepatocellular carcinoma metastasis.",

author = "Harn, {H. J.} and Ho, {L. I.} and Yu, {C. P.} and Wang, {M. W.} and Lee, {H. S.} and Lin, {J. J.} and Lee, {W. H.} and Isola, {N. R.} and Cooper, {D. L.}",

year = "1994",

language = "English",

volume = "32",

pages = "233--238",

journal = "Biochemistry and Molecular Biology International",

issn = "1069-8302",

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T1 - The variant mRNA isoform of human metastasis gene (CD44V) detected in the cell lines of human hepatocellular carcinoma

AU - Harn, H. J.

AU - Ho, L. I.

AU - Yu, C. P.

AU - Wang, M. W.

AU - Lee, H. S.

AU - Lin, J. J.

AU - Lee, W. H.

AU - Isola, N. R.

AU - Cooper, D. L.

PY - 1994

Y1 - 1994

N2 - The interaction of the cell surface receptor CD44 molecular with its ligands (addressin, extracellular matrix etc.,) plays an important role in fulfilling the lymphocyte homing and immune reaction. Recently alternatively spliced products of CD44 gene are found to be involved in tumor metastasis as well. Our report found that CD44 prototype RNA (CD44S) was present in all five tumor cell lines. Isoform CD44 RNA (CD44V) was recognized in three metastasized hepatocellular carcinoma cell lines, J5, HCC36, HEP38. In addition, the J5 CD44 RNA isoform expressed two distinct trancripts which are of the same size as MDA-231 breast tumor cell line. The MDA-231 CD44 RNA variant (CD44V) has been confirmed to contain metastasis domain 4 and 5. it is implicated that the alternative RNA splicing may also play a major role in hepatocellular carcinoma metastasis.

AB - The interaction of the cell surface receptor CD44 molecular with its ligands (addressin, extracellular matrix etc.,) plays an important role in fulfilling the lymphocyte homing and immune reaction. Recently alternatively spliced products of CD44 gene are found to be involved in tumor metastasis as well. Our report found that CD44 prototype RNA (CD44S) was present in all five tumor cell lines. Isoform CD44 RNA (CD44V) was recognized in three metastasized hepatocellular carcinoma cell lines, J5, HCC36, HEP38. In addition, the J5 CD44 RNA isoform expressed two distinct trancripts which are of the same size as MDA-231 breast tumor cell line. The MDA-231 CD44 RNA variant (CD44V) has been confirmed to contain metastasis domain 4 and 5. it is implicated that the alternative RNA splicing may also play a major role in hepatocellular carcinoma metastasis.

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JF - Biochemistry and Molecular Biology International

IS - 2

ER -

The variant mRNA isoform of human metastasis gene (CD44V) detected in the cell lines of human hepatocellular carcinoma

Abstract

ASJC Scopus subject areas

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