The use of intensity-based Doppler variance method for single vessel response to functional neurovascular activation

Wen Chuan Kuo, Yue Ming Kuo, Jia Pu Syu, Han Lin Wang, Chi Ming Lai, Jia Wei Chen, Yu Chun Lo, You Yin Chen

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)


This study presents 1 use of optical coherence tomography (OCT) angiography technique to examine neurovascular coupling effect. Repeated B-scans OCT recording is performed on the rat somatosensory cortex with cranial window preparation while its contralateral forepaw is electrically stimulated to activate the neurons in rest. We use an intensity-based Doppler variance (IBDV) algorithm mapped cerebral blood vessels in the cortex, and the temporal alteration in blood perfusion during neurovascular activation is analyzed using the proposed IBDV quantitative parameters. By using principal component analysis-based Fuzzy C Means clustering method, the stimulus-evoked vasomotion patterns were classified into 3 categories. We found that the response time of small vessels (resting diameter 14.9 ±6.6 μm), middle vessels (resting diameter 21.1 ±7.9 μm) and large vessels (resting diameter 50.7 ±6.5 μm) to achieve 5% change of vascular dilation after stimulation was 1.5, 2 and 5.5 seconds, respectively. Approximately 5% peak change of relative blood flow (RBF) in both small and middle vessels was observed. The large vessels react slowly and their responses nearly 4 seconds delayed, but no significant change in RBF of the large vessels was seen.

Original languageEnglish
Article numbere201800017
JournalJournal of Biophotonics
Issue number7
Publication statusPublished - Jul 1 2018


  • angiography
  • intensity-based Doppler variance
  • neurovascular activation
  • optical coherence tomography

ASJC Scopus subject areas

  • Chemistry(all)
  • Materials Science(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Engineering(all)
  • Physics and Astronomy(all)


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