The ultrasensitive silver "protein" stain also detects nanograms of nucleic acids

Laura L. Somerville; Kuan Wang

doi:10.1016/0006-291X(81)91487-X

The ultrasensitive silver "protein" stain also detects nanograms of nucleic acids

Laura L. Somerville, Kuan Wang

Research output: Contribution to journal › Article › peer-review

73 Citations (Scopus)

Abstract

The ultrasensitive silver staining procedure developed for proteins also stains nanogram quantities of RNA and DNA in polyacrylamide gels. A gradient polyacrylamide gel system is described which separates proteins from 10⁴ to 10⁶ M_r, RNA from 5S to 23S and DNA from 0.4 to 21 Kb. The sensitivity of nucleic acid silver staining in this gel system considerably exceeds that of commonly used DNA and RNA dye-binding stains.

Original language	English
Pages (from-to)	53-58
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	102
Issue number	1
DOIs	https://doi.org/10.1016/0006-291X(81)91487-X
Publication status	Published - Sept 16 1981
Externally published	Yes

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/0006-291X(81)91487-X

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abstract = "The ultrasensitive silver staining procedure developed for proteins also stains nanogram quantities of RNA and DNA in polyacrylamide gels. A gradient polyacrylamide gel system is described which separates proteins from 104 to 106 Mr, RNA from 5S to 23S and DNA from 0.4 to 21 Kb. The sensitivity of nucleic acid silver staining in this gel system considerably exceeds that of commonly used DNA and RNA dye-binding stains.",

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AB - The ultrasensitive silver staining procedure developed for proteins also stains nanogram quantities of RNA and DNA in polyacrylamide gels. A gradient polyacrylamide gel system is described which separates proteins from 104 to 106 Mr, RNA from 5S to 23S and DNA from 0.4 to 21 Kb. The sensitivity of nucleic acid silver staining in this gel system considerably exceeds that of commonly used DNA and RNA dye-binding stains.

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The ultrasensitive silver "protein" stain also detects nanograms of nucleic acids

Abstract

ASJC Scopus subject areas

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