The tea polyphenols EGCG and EGC repress mRNA expression of human telomerase reverse transcriptase (hTERT) in carcinoma cells

Shu Chun Lin; Wan Chun Li; Jing Wen Shih; Kuo Fu Hong; Yen Ru Pan; Jing Jer Lin

doi:10.1016/j.canlet.2005.05.003

The tea polyphenols EGCG and EGC repress mRNA expression of human telomerase reverse transcriptase (hTERT) in carcinoma cells

Shu Chun Lin
, Wan Chun Li
, Jing Wen Shih
, Kuo Fu Hong
, Yen Ru Pan
, Jing Jer Lin

Research output: Contribution to journal › Article › peer-review

43 Citations (Scopus)

Abstract

Tea polyphenols have inhibitive effects for carcinogenesis. A reporter system controlled by hTERT promoter was constructed to evaluate the effects of tea polyphenols, (-)-epigallocatechin-3-gallate (EGCG) and (-)-epigallocatechin (EGC) on the repression of hTERT transcription. The hTERT promoter activity was selectively repressed by 20-40 μM EGCG and EGC in a dose- and time-dependent manner. Real-time RT-PCR confirmed that the endogenous hTERT mRNA level was decreased in H1299, OECM-1 and SAS cells treated with EGCG or EGC. Our results identified the repression activities of EGCG and EGC toward telomerase expression that might be linked to inhibition of carcinoma cell growth. This cell-based reporter system is useful for screening drugs targeting hTERT repression.

Original language	English
Pages (from-to)	80-88
Number of pages	9
Journal	Cancer Letters
Volume	236
Issue number	1
DOIs	https://doi.org/10.1016/j.canlet.2005.05.003
Publication status	Published - May 8 2006
Externally published	Yes

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

EGC
EGCG
Neoplasm
Tea
Telomerase
hTERT

ASJC Scopus subject areas

Oncology
Cancer Research

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10.1016/j.canlet.2005.05.003

Cite this

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title = "The tea polyphenols EGCG and EGC repress mRNA expression of human telomerase reverse transcriptase (hTERT) in carcinoma cells",

abstract = "Tea polyphenols have inhibitive effects for carcinogenesis. A reporter system controlled by hTERT promoter was constructed to evaluate the effects of tea polyphenols, (-)-epigallocatechin-3-gallate (EGCG) and (-)-epigallocatechin (EGC) on the repression of hTERT transcription. The hTERT promoter activity was selectively repressed by 20-40 μM EGCG and EGC in a dose- and time-dependent manner. Real-time RT-PCR confirmed that the endogenous hTERT mRNA level was decreased in H1299, OECM-1 and SAS cells treated with EGCG or EGC. Our results identified the repression activities of EGCG and EGC toward telomerase expression that might be linked to inhibition of carcinoma cell growth. This cell-based reporter system is useful for screening drugs targeting hTERT repression.",

keywords = "EGC, EGCG, Neoplasm, Tea, Telomerase, hTERT",

author = "Lin, \{Shu Chun\} and Li, \{Wan Chun\} and Shih, \{Jing Wen\} and Hong, \{Kuo Fu\} and Pan, \{Yen Ru\} and Lin, \{Jing Jer\}",

year = "2006",

month = may,

day = "8",

doi = "10.1016/j.canlet.2005.05.003",

language = "English",

volume = "236",

pages = "80--88",

journal = "Cancer Letters",

issn = "0304-3835",

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T1 - The tea polyphenols EGCG and EGC repress mRNA expression of human telomerase reverse transcriptase (hTERT) in carcinoma cells

AU - Lin, Shu Chun

AU - Li, Wan Chun

AU - Shih, Jing Wen

AU - Hong, Kuo Fu

AU - Pan, Yen Ru

AU - Lin, Jing Jer

PY - 2006/5/8

Y1 - 2006/5/8

N2 - Tea polyphenols have inhibitive effects for carcinogenesis. A reporter system controlled by hTERT promoter was constructed to evaluate the effects of tea polyphenols, (-)-epigallocatechin-3-gallate (EGCG) and (-)-epigallocatechin (EGC) on the repression of hTERT transcription. The hTERT promoter activity was selectively repressed by 20-40 μM EGCG and EGC in a dose- and time-dependent manner. Real-time RT-PCR confirmed that the endogenous hTERT mRNA level was decreased in H1299, OECM-1 and SAS cells treated with EGCG or EGC. Our results identified the repression activities of EGCG and EGC toward telomerase expression that might be linked to inhibition of carcinoma cell growth. This cell-based reporter system is useful for screening drugs targeting hTERT repression.

AB - Tea polyphenols have inhibitive effects for carcinogenesis. A reporter system controlled by hTERT promoter was constructed to evaluate the effects of tea polyphenols, (-)-epigallocatechin-3-gallate (EGCG) and (-)-epigallocatechin (EGC) on the repression of hTERT transcription. The hTERT promoter activity was selectively repressed by 20-40 μM EGCG and EGC in a dose- and time-dependent manner. Real-time RT-PCR confirmed that the endogenous hTERT mRNA level was decreased in H1299, OECM-1 and SAS cells treated with EGCG or EGC. Our results identified the repression activities of EGCG and EGC toward telomerase expression that might be linked to inhibition of carcinoma cell growth. This cell-based reporter system is useful for screening drugs targeting hTERT repression.

KW - EGC

KW - EGCG

KW - Neoplasm

KW - Tea

KW - Telomerase

KW - hTERT

UR - https://www.scopus.com/pages/publications/33646526291

UR - https://www.scopus.com/pages/publications/33646526291#tab=citedBy

U2 - 10.1016/j.canlet.2005.05.003

DO - 10.1016/j.canlet.2005.05.003

M3 - Article

C2 - 15975707

AN - SCOPUS:33646526291

SN - 0304-3835

VL - 236

SP - 80

EP - 88

JO - Cancer Letters

JF - Cancer Letters

IS - 1

ER -

The tea polyphenols EGCG and EGC repress mRNA expression of human telomerase reverse transcriptase (hTERT) in carcinoma cells

Abstract

UN SDGs

Keywords

ASJC Scopus subject areas

Access to Document

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