TY - JOUR
T1 - The soybean bioactive peptide VHVV alleviates hypertension-induced renal damage in hypertensive rats via the SIRT1-PGC1α/Nrf2 pathway
AU - Tsai, Bruce Chi Kang
AU - Kuo, Wei Wen
AU - Day, Cecilia Hsuan
AU - Hsieh, Dennis Jine Yuan
AU - Kuo, Chia Hua
AU - Daddam, Jayasimharayalu
AU - Chen, Ray Jade
AU - Padma, V. Vijaya
AU - Wang, Guiqing
AU - Huang, Chih Yang
N1 - Funding Information:
This research was supported by grants from China Medical University, Asia University and China Medical University Hospital (CMU107-ASIA-03, ASIA-106-CMUH-02, ASIA-107-CMUH-02, and DMR-108-143). We would like to acknowledge Dr. Shanmugam Tamilselvi and Dr. Wan-Teng Lin, who provided insight and expertise that greatly assisted the research, and Dr. B. Mahalakshmi, who provided suggestions during the writing of this manuscript draft. We also thank American Journal Experts for English editing assistance.
Publisher Copyright:
© 2020
PY - 2020/12
Y1 - 2020/12
N2 - Hypertension triggers oxidative stress, causing dysfunction in organs, including the brain, heart, and kidney. In this study, we examined the potential renal benefit of the soybean bioactive peptide VHVV by in silico analysis and in spontaneously hypertensive rats. Sixteen-week-old spontaneously hypertensive rats were divided into 3 groups and treated with or without VHVV or captopril. Age-matched Wistar-Kyoto rats were used as normotensive control group. The rats were sacrificed after 8 weeks of treatment, and kidneys were analyzed to demonstrate the efficacy of VHVV. In silico analysis confirmed the antihypertensive activity of VHVV. In animal study, VHVV induced antioxidant defense and modulated mitochondrial homeostasis in the hypertensive kidney via SIRT1-PGC1α/Nrf2 pathway. Hence, improvements in renal cells were attributed to the suppression of inflammation and apoptosis, and the architecture of the hypertensive kidney was restored after peptide treatment. Overall, VHVV exhibited therapeutic effects by decreasing renal damage caused by hypertension-induced free radicals.
AB - Hypertension triggers oxidative stress, causing dysfunction in organs, including the brain, heart, and kidney. In this study, we examined the potential renal benefit of the soybean bioactive peptide VHVV by in silico analysis and in spontaneously hypertensive rats. Sixteen-week-old spontaneously hypertensive rats were divided into 3 groups and treated with or without VHVV or captopril. Age-matched Wistar-Kyoto rats were used as normotensive control group. The rats were sacrificed after 8 weeks of treatment, and kidneys were analyzed to demonstrate the efficacy of VHVV. In silico analysis confirmed the antihypertensive activity of VHVV. In animal study, VHVV induced antioxidant defense and modulated mitochondrial homeostasis in the hypertensive kidney via SIRT1-PGC1α/Nrf2 pathway. Hence, improvements in renal cells were attributed to the suppression of inflammation and apoptosis, and the architecture of the hypertensive kidney was restored after peptide treatment. Overall, VHVV exhibited therapeutic effects by decreasing renal damage caused by hypertension-induced free radicals.
KW - Hypertension
KW - Kidney
KW - Nrf2
KW - PGC1α
KW - SIRT1
KW - Soybean peptide
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U2 - 10.1016/j.jff.2020.104255
DO - 10.1016/j.jff.2020.104255
M3 - Article
AN - SCOPUS:85094842091
SN - 1756-4646
VL - 75
JO - Journal of Functional Foods
JF - Journal of Functional Foods
M1 - 104255
ER -