The role of the calmodulin-dependent pathway in static magnetic field-induced mechanotransduction

Jen Chang Yang; Sheng Yang Lee; Chi An Chen; Che Tong Lin; Chang Chih Chen; Haw Ming Huang

doi:10.1002/bem.20559

The role of the calmodulin-dependent pathway in static magnetic field-induced mechanotransduction

Jen Chang Yang, Sheng Yang Lee, Chi An Chen, Che Tong Lin, Chang Chih Chen, Haw Ming Huang

Research output: Contribution to journal › Article › peer-review

13 Citations (Scopus)

Abstract

While the effects of static magnetic fields (SMFs) on osteoblastic differentiation are well demonstrated, the mechanotransduction pathways of SMFs are still unclear. The aim of this study was to explore the role of calmodulin in the biophysical effects of SMFs on osteoblastic cells. MG63 cells were exposed to a 0.4 T SMF The expression of phosphodiesterase RNA in the cytoplasm was tested using real-time polymerase chain reaction. The differentiation of the cells was assessed by detecting changes in alkaline phosphatase activity. The role of calmodulin antagonist W-7 was used to evaluate alterations in osteoblastic proliferation and differentiation after the SMF simulations. Our results showed that SMF exposure increased alkaline phosphatase activity and phosphodiesterase 1C gene expression in MG63 cells. Addition of W-7 significantly inhibited the SMF-induced cellular response. We suggest that one possible mechanism by which SMFs affects osteoblastic maturation is through a calmodulin-dependent mechanotransduction pathway.

Original language	English
Pages (from-to)	255-261
Number of pages	7
Journal	Bioelectromagnetics
Volume	31
Issue number	4
DOIs	https://doi.org/10.1002/bem.20559
Publication status	Published - May 2010

Keywords

Calmodulin
Mechanobiology
Osteoblast
Phosphodiesterase
Static magnetic field

ASJC Scopus subject areas

Biophysics
Radiology Nuclear Medicine and imaging
Physiology

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10.1002/bem.20559

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title = "The role of the calmodulin-dependent pathway in static magnetic field-induced mechanotransduction",

abstract = "While the effects of static magnetic fields (SMFs) on osteoblastic differentiation are well demonstrated, the mechanotransduction pathways of SMFs are still unclear. The aim of this study was to explore the role of calmodulin in the biophysical effects of SMFs on osteoblastic cells. MG63 cells were exposed to a 0.4 T SMF The expression of phosphodiesterase RNA in the cytoplasm was tested using real-time polymerase chain reaction. The differentiation of the cells was assessed by detecting changes in alkaline phosphatase activity. The role of calmodulin antagonist W-7 was used to evaluate alterations in osteoblastic proliferation and differentiation after the SMF simulations. Our results showed that SMF exposure increased alkaline phosphatase activity and phosphodiesterase 1C gene expression in MG63 cells. Addition of W-7 significantly inhibited the SMF-induced cellular response. We suggest that one possible mechanism by which SMFs affects osteoblastic maturation is through a calmodulin-dependent mechanotransduction pathway.",

keywords = "Calmodulin, Mechanobiology, Osteoblast, Phosphodiesterase, Static magnetic field",

author = "Yang, {Jen Chang} and Lee, {Sheng Yang} and Chen, {Chi An} and Lin, {Che Tong} and Chen, {Chang Chih} and Huang, {Haw Ming}",

year = "2010",

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AU - Yang, Jen Chang

AU - Lee, Sheng Yang

AU - Chen, Chi An

AU - Lin, Che Tong

AU - Chen, Chang Chih

AU - Huang, Haw Ming

PY - 2010/5

Y1 - 2010/5

N2 - While the effects of static magnetic fields (SMFs) on osteoblastic differentiation are well demonstrated, the mechanotransduction pathways of SMFs are still unclear. The aim of this study was to explore the role of calmodulin in the biophysical effects of SMFs on osteoblastic cells. MG63 cells were exposed to a 0.4 T SMF The expression of phosphodiesterase RNA in the cytoplasm was tested using real-time polymerase chain reaction. The differentiation of the cells was assessed by detecting changes in alkaline phosphatase activity. The role of calmodulin antagonist W-7 was used to evaluate alterations in osteoblastic proliferation and differentiation after the SMF simulations. Our results showed that SMF exposure increased alkaline phosphatase activity and phosphodiesterase 1C gene expression in MG63 cells. Addition of W-7 significantly inhibited the SMF-induced cellular response. We suggest that one possible mechanism by which SMFs affects osteoblastic maturation is through a calmodulin-dependent mechanotransduction pathway.

AB - While the effects of static magnetic fields (SMFs) on osteoblastic differentiation are well demonstrated, the mechanotransduction pathways of SMFs are still unclear. The aim of this study was to explore the role of calmodulin in the biophysical effects of SMFs on osteoblastic cells. MG63 cells were exposed to a 0.4 T SMF The expression of phosphodiesterase RNA in the cytoplasm was tested using real-time polymerase chain reaction. The differentiation of the cells was assessed by detecting changes in alkaline phosphatase activity. The role of calmodulin antagonist W-7 was used to evaluate alterations in osteoblastic proliferation and differentiation after the SMF simulations. Our results showed that SMF exposure increased alkaline phosphatase activity and phosphodiesterase 1C gene expression in MG63 cells. Addition of W-7 significantly inhibited the SMF-induced cellular response. We suggest that one possible mechanism by which SMFs affects osteoblastic maturation is through a calmodulin-dependent mechanotransduction pathway.

KW - Calmodulin

KW - Mechanobiology

KW - Osteoblast

KW - Phosphodiesterase

KW - Static magnetic field

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The role of the calmodulin-dependent pathway in static magnetic field-induced mechanotransduction

Abstract

Keywords

ASJC Scopus subject areas

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