The role of α2,3-linked sialylation on clear cell type epithelial ovarian cancer

Pi-Lin Sung; Kuo-Chang Wen; Huann-Cheng Horng; Chia-Ming Chang; Yi-Jen Chen; Wen-Ling Lee; Peng-Hui Wang

doi:10.1016/j.tjog.2018.02.015

The role of α2,3-linked sialylation on clear cell type epithelial ovarian cancer

Pi-Lin Sung, Kuo-Chang Wen, Huann-Cheng Horng, Chia-Ming Chang, Yi-Jen Chen, Wen-Ling Lee, Peng-Hui Wang

Research output: Contribution to journal › Article › peer-review

30 Citations (Scopus)

Abstract

OBJECTIVE: Our previous study has shown that high expression of α2,3-sialytransferase type I was associated with advanced stage serous type epithelial ovarian cancer (EOC). The aim of the current study further attempts to evaluate the altered α 2,3-sialylation on the behavior of clear cell type EOC (C-EOC).

MATERIALS AND METHODS: Immunohistochemistry staining, bioinformatics analysis and tissue array were used to disclose the clinical significance of over α2,3-sialylation in C-EOC. An α2,3 sialylation inhibitor, soyasaponin I (SsaI) was used to investigate the behavior change of the C-EOC cell line.

RESULTS: We reconfirmed that α2,3-sialylation, instead of α2,6- sialylation, was associated with late-stage C-EOC. Soyasaponin I could inhibit α2,3-sialylation of C-EOC cell lines and increase E-cadherin expression with subsequently suppressing migration of C-EOC cells.

CONCLUSIONS: The current study demonstrated the important role of α2,3-linked sialylation in C-EOC and targeting of α2,3-linked sialylation might offer as a potential therapeutic strategy in the future.

Original language	English
Pages (from-to)	255-263
Number of pages	9
Journal	Taiwanese Journal of Obstetrics and Gynecology
Volume	57
Issue number	2
DOIs	https://doi.org/10.1016/j.tjog.2018.02.015
Publication status	Published - Apr 2018
Externally published	Yes

Keywords

Adenocarcinoma, Clear Cell/genetics
Animals
Cadherins/genetics
Carcinoma, Ovarian Epithelial
Cell Line, Tumor
Cystadenocarcinoma, Serous/genetics
Female
Gene Expression/drug effects
Gene Expression Regulation, Neoplastic/drug effects
Humans
Mice
Neoplasms, Glandular and Epithelial/genetics
Oleanolic Acid/analogs & derivatives
Ovarian Neoplasms/genetics
Saponins/pharmacology
Sialyltransferases/antagonists & inhibitors
beta-Galactoside alpha-2,3-Sialyltransferase

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1016/j.tjog.2018.02.015

Cite this

@article{7003716ff90a45a6ba55f7e721a82066,

title = "The role of α2,3-linked sialylation on clear cell type epithelial ovarian cancer",

abstract = "OBJECTIVE: Our previous study has shown that high expression of α2,3-sialytransferase type I was associated with advanced stage serous type epithelial ovarian cancer (EOC). The aim of the current study further attempts to evaluate the altered α 2,3-sialylation on the behavior of clear cell type EOC (C-EOC).MATERIALS AND METHODS: Immunohistochemistry staining, bioinformatics analysis and tissue array were used to disclose the clinical significance of over α2,3-sialylation in C-EOC. An α2,3 sialylation inhibitor, soyasaponin I (SsaI) was used to investigate the behavior change of the C-EOC cell line.RESULTS: We reconfirmed that α2,3-sialylation, instead of α2,6- sialylation, was associated with late-stage C-EOC. Soyasaponin I could inhibit α2,3-sialylation of C-EOC cell lines and increase E-cadherin expression with subsequently suppressing migration of C-EOC cells.CONCLUSIONS: The current study demonstrated the important role of α2,3-linked sialylation in C-EOC and targeting of α2,3-linked sialylation might offer as a potential therapeutic strategy in the future.",

keywords = "Adenocarcinoma, Clear Cell/genetics, Animals, Cadherins/genetics, Carcinoma, Ovarian Epithelial, Cell Line, Tumor, Cystadenocarcinoma, Serous/genetics, Female, Gene Expression/drug effects, Gene Expression Regulation, Neoplastic/drug effects, Humans, Mice, Neoplasms, Glandular and Epithelial/genetics, Oleanolic Acid/analogs & derivatives, Ovarian Neoplasms/genetics, Saponins/pharmacology, Sialyltransferases/antagonists & inhibitors, beta-Galactoside alpha-2,3-Sialyltransferase",

author = "Pi-Lin Sung and Kuo-Chang Wen and Huann-Cheng Horng and Chia-Ming Chang and Yi-Jen Chen and Wen-Ling Lee and Peng-Hui Wang",

note = "Copyright {\textcopyright} 2018. Published by Elsevier B.V.",

year = "2018",

month = apr,

doi = "10.1016/j.tjog.2018.02.015",

language = "English",

volume = "57",

pages = "255--263",

journal = "Taiwanese Journal of Obstetrics and Gynecology",

issn = "1028-4559",

publisher = "臺灣婦產科醫學會",

number = "2",

}

TY - JOUR

T1 - The role of α2,3-linked sialylation on clear cell type epithelial ovarian cancer

AU - Sung, Pi-Lin

AU - Wen, Kuo-Chang

AU - Horng, Huann-Cheng

AU - Chang, Chia-Ming

AU - Chen, Yi-Jen

AU - Lee, Wen-Ling

AU - Wang, Peng-Hui

PY - 2018/4

Y1 - 2018/4

N2 - OBJECTIVE: Our previous study has shown that high expression of α2,3-sialytransferase type I was associated with advanced stage serous type epithelial ovarian cancer (EOC). The aim of the current study further attempts to evaluate the altered α 2,3-sialylation on the behavior of clear cell type EOC (C-EOC).MATERIALS AND METHODS: Immunohistochemistry staining, bioinformatics analysis and tissue array were used to disclose the clinical significance of over α2,3-sialylation in C-EOC. An α2,3 sialylation inhibitor, soyasaponin I (SsaI) was used to investigate the behavior change of the C-EOC cell line.RESULTS: We reconfirmed that α2,3-sialylation, instead of α2,6- sialylation, was associated with late-stage C-EOC. Soyasaponin I could inhibit α2,3-sialylation of C-EOC cell lines and increase E-cadherin expression with subsequently suppressing migration of C-EOC cells.CONCLUSIONS: The current study demonstrated the important role of α2,3-linked sialylation in C-EOC and targeting of α2,3-linked sialylation might offer as a potential therapeutic strategy in the future.

AB - OBJECTIVE: Our previous study has shown that high expression of α2,3-sialytransferase type I was associated with advanced stage serous type epithelial ovarian cancer (EOC). The aim of the current study further attempts to evaluate the altered α 2,3-sialylation on the behavior of clear cell type EOC (C-EOC).MATERIALS AND METHODS: Immunohistochemistry staining, bioinformatics analysis and tissue array were used to disclose the clinical significance of over α2,3-sialylation in C-EOC. An α2,3 sialylation inhibitor, soyasaponin I (SsaI) was used to investigate the behavior change of the C-EOC cell line.RESULTS: We reconfirmed that α2,3-sialylation, instead of α2,6- sialylation, was associated with late-stage C-EOC. Soyasaponin I could inhibit α2,3-sialylation of C-EOC cell lines and increase E-cadherin expression with subsequently suppressing migration of C-EOC cells.CONCLUSIONS: The current study demonstrated the important role of α2,3-linked sialylation in C-EOC and targeting of α2,3-linked sialylation might offer as a potential therapeutic strategy in the future.

KW - Adenocarcinoma, Clear Cell/genetics

KW - Animals

KW - Cadherins/genetics

KW - Carcinoma, Ovarian Epithelial

KW - Cell Line, Tumor

KW - Cystadenocarcinoma, Serous/genetics

KW - Female

KW - Gene Expression/drug effects

KW - Gene Expression Regulation, Neoplastic/drug effects

KW - Humans

KW - Mice

KW - Neoplasms, Glandular and Epithelial/genetics

KW - Oleanolic Acid/analogs & derivatives

KW - Ovarian Neoplasms/genetics

KW - Saponins/pharmacology

KW - Sialyltransferases/antagonists & inhibitors

KW - beta-Galactoside alpha-2,3-Sialyltransferase

U2 - 10.1016/j.tjog.2018.02.015

DO - 10.1016/j.tjog.2018.02.015

M3 - Article

C2 - 29673670

SN - 1028-4559

VL - 57

SP - 255

EP - 263

JO - Taiwanese Journal of Obstetrics and Gynecology

JF - Taiwanese Journal of Obstetrics and Gynecology

IS - 2

ER -

The role of α2,3-linked sialylation on clear cell type epithelial ovarian cancer

Abstract

Keywords

UN SDGs

Access to Document

Fingerprint

Cite this