The relation between NEAT1 expression level and survival rate in patients with oral squamous cell carcinoma

Nan Chin Lin; Shih Min Hsia; Tong Hong Wang; Po Jung Li; Yu Hsin Tseng; Kuo Chou Chiu; Hsi Feng Tu; Yin Hwa Shih; Tzong Ming Shieh

doi:10.1016/j.jds.2021.09.018

The relation between NEAT1 expression level and survival rate in patients with oral squamous cell carcinoma

Nan Chin Lin, Shih Min Hsia, Tong Hong Wang, Po Jung Li, Yu Hsin Tseng, Kuo Chou Chiu, Hsi Feng Tu, Yin Hwa Shih, Tzong Ming Shieh

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

Background/purpose: Numerous studies have shown that long noncoding RNAs (lncRNAs) are involved in cancer progression and chemotherapy resistance. Nuclear enriched abundant transcript 1 (NEAT1) is an lncRNA. It affects tumor cell progression and drug resistance in various tumors. However, the relation of NEAT1 and survival rate in oral squamous cell carcinoma (OSCC) requires further study. Materials and methods: One normal gingival epithelium cell line, SG, three oral cancer cell lines (HSC3, OEC-M1, and SAS), 34 paired non-cancerous matched tissues (NCMT), and OSCC tissues were used in this study. Tri-reagent was used for total RNA extraction. NEAT1 expression was assessed by reverse transcription-quantitative PCR (RT-qPCR). Results: NEAT1 expression in oral cancer cell lines was lower than that in normal cells and was significantly downregulated in OSCC. NEAT1 upregulation reduced the survival rate of patients with OSCC. NEAT1 upregulation also reduced the survival rate of OSCC patients treated with chemotherapy and radiotherapy. Conclusion: These results indicate that NEAT1 expression is a valuable biomarker for the prediction and prognosis of oral cancer.

Original language	English
Pages (from-to)	361-367
Number of pages	7
Journal	Journal of Dental Sciences
Volume	17
Issue number	1
DOIs	https://doi.org/10.1016/j.jds.2021.09.018
Publication status	Published - Jan 2022

Keywords

Chemotherapy
Nuclear enriched abundant transcript 1 (NEAT1)
Oral squamous cell carcinoma
Radiotherapy
Survival rate

ASJC Scopus subject areas

Dentistry(all)

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.jds.2021.09.018

Cite this

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title = "The relation between NEAT1 expression level and survival rate in patients with oral squamous cell carcinoma",

abstract = "Background/purpose: Numerous studies have shown that long noncoding RNAs (lncRNAs) are involved in cancer progression and chemotherapy resistance. Nuclear enriched abundant transcript 1 (NEAT1) is an lncRNA. It affects tumor cell progression and drug resistance in various tumors. However, the relation of NEAT1 and survival rate in oral squamous cell carcinoma (OSCC) requires further study. Materials and methods: One normal gingival epithelium cell line, SG, three oral cancer cell lines (HSC3, OEC-M1, and SAS), 34 paired non-cancerous matched tissues (NCMT), and OSCC tissues were used in this study. Tri-reagent was used for total RNA extraction. NEAT1 expression was assessed by reverse transcription-quantitative PCR (RT-qPCR). Results: NEAT1 expression in oral cancer cell lines was lower than that in normal cells and was significantly downregulated in OSCC. NEAT1 upregulation reduced the survival rate of patients with OSCC. NEAT1 upregulation also reduced the survival rate of OSCC patients treated with chemotherapy and radiotherapy. Conclusion: These results indicate that NEAT1 expression is a valuable biomarker for the prediction and prognosis of oral cancer.",

keywords = "Chemotherapy, Nuclear enriched abundant transcript 1 (NEAT1), Oral squamous cell carcinoma, Radiotherapy, Survival rate",

author = "Lin, {Nan Chin} and Hsia, {Shih Min} and Wang, {Tong Hong} and Li, {Po Jung} and Tseng, {Yu Hsin} and Chiu, {Kuo Chou} and Tu, {Hsi Feng} and Shih, {Yin Hwa} and Shieh, {Tzong Ming}",

note = "Funding Information: Experiments and data analysis were performed in part through the use of the Medical Research Core Facilities Center, Office of Research & Development at China medical University, Taichung, Taiwan, R.O.C. This study was supported by grants from China Medical University ( CMU109-S-17 , CMU109-MF-32 ) and the Ministry of Science and Technology , R.O.C. ( MOST 108-2314-B-039-009-MY3 ). Funding Information: Experiments and data analysis were performed in part through the use of the Medical Research Core Facilities Center, Office of Research & Development at China medical University, Taichung, Taiwan, R.O.C. This study was supported by grants from China Medical University (CMU109-S-17, CMU109-MF-32) and the Ministry of Science and Technology, R.O.C. (MOST 108-2314-B-039-009-MY3). Publisher Copyright: {\textcopyright} 2021 Association for Dental Sciences of the Republic of China",

year = "2022",

month = jan,

doi = "10.1016/j.jds.2021.09.018",

language = "English",

volume = "17",

pages = "361--367",

journal = "Journal of Dental Sciences",

issn = "1991-7902",

publisher = "中華牙醫學會",

number = "1",

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TY - JOUR

T1 - The relation between NEAT1 expression level and survival rate in patients with oral squamous cell carcinoma

AU - Lin, Nan Chin

AU - Hsia, Shih Min

AU - Wang, Tong Hong

AU - Li, Po Jung

AU - Tseng, Yu Hsin

AU - Chiu, Kuo Chou

AU - Tu, Hsi Feng

AU - Shih, Yin Hwa

AU - Shieh, Tzong Ming

N1 - Funding Information: Experiments and data analysis were performed in part through the use of the Medical Research Core Facilities Center, Office of Research & Development at China medical University, Taichung, Taiwan, R.O.C. This study was supported by grants from China Medical University ( CMU109-S-17 , CMU109-MF-32 ) and the Ministry of Science and Technology , R.O.C. ( MOST 108-2314-B-039-009-MY3 ). Funding Information: Experiments and data analysis were performed in part through the use of the Medical Research Core Facilities Center, Office of Research & Development at China medical University, Taichung, Taiwan, R.O.C. This study was supported by grants from China Medical University (CMU109-S-17, CMU109-MF-32) and the Ministry of Science and Technology, R.O.C. (MOST 108-2314-B-039-009-MY3). Publisher Copyright: © 2021 Association for Dental Sciences of the Republic of China

PY - 2022/1

Y1 - 2022/1

N2 - Background/purpose: Numerous studies have shown that long noncoding RNAs (lncRNAs) are involved in cancer progression and chemotherapy resistance. Nuclear enriched abundant transcript 1 (NEAT1) is an lncRNA. It affects tumor cell progression and drug resistance in various tumors. However, the relation of NEAT1 and survival rate in oral squamous cell carcinoma (OSCC) requires further study. Materials and methods: One normal gingival epithelium cell line, SG, three oral cancer cell lines (HSC3, OEC-M1, and SAS), 34 paired non-cancerous matched tissues (NCMT), and OSCC tissues were used in this study. Tri-reagent was used for total RNA extraction. NEAT1 expression was assessed by reverse transcription-quantitative PCR (RT-qPCR). Results: NEAT1 expression in oral cancer cell lines was lower than that in normal cells and was significantly downregulated in OSCC. NEAT1 upregulation reduced the survival rate of patients with OSCC. NEAT1 upregulation also reduced the survival rate of OSCC patients treated with chemotherapy and radiotherapy. Conclusion: These results indicate that NEAT1 expression is a valuable biomarker for the prediction and prognosis of oral cancer.

AB - Background/purpose: Numerous studies have shown that long noncoding RNAs (lncRNAs) are involved in cancer progression and chemotherapy resistance. Nuclear enriched abundant transcript 1 (NEAT1) is an lncRNA. It affects tumor cell progression and drug resistance in various tumors. However, the relation of NEAT1 and survival rate in oral squamous cell carcinoma (OSCC) requires further study. Materials and methods: One normal gingival epithelium cell line, SG, three oral cancer cell lines (HSC3, OEC-M1, and SAS), 34 paired non-cancerous matched tissues (NCMT), and OSCC tissues were used in this study. Tri-reagent was used for total RNA extraction. NEAT1 expression was assessed by reverse transcription-quantitative PCR (RT-qPCR). Results: NEAT1 expression in oral cancer cell lines was lower than that in normal cells and was significantly downregulated in OSCC. NEAT1 upregulation reduced the survival rate of patients with OSCC. NEAT1 upregulation also reduced the survival rate of OSCC patients treated with chemotherapy and radiotherapy. Conclusion: These results indicate that NEAT1 expression is a valuable biomarker for the prediction and prognosis of oral cancer.

KW - Chemotherapy

KW - Nuclear enriched abundant transcript 1 (NEAT1)

KW - Oral squamous cell carcinoma

KW - Radiotherapy

KW - Survival rate

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DO - 10.1016/j.jds.2021.09.018

M3 - Article

AN - SCOPUS:85115762810

SN - 1991-7902

VL - 17

SP - 361

EP - 367

JO - Journal of Dental Sciences

JF - Journal of Dental Sciences

IS - 1

ER -

The relation between NEAT1 expression level and survival rate in patients with oral squamous cell carcinoma

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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