TY - JOUR
T1 - The raman spectra of left-handed DNA oligomers incorporating adenine-thymine base pairs
AU - Benevides, J. M.
AU - Wang, A. H.J.
AU - Van Der Marel, G. A.
AU - van Boom, J. H.
AU - Rich, A.
AU - Thomas, G. J.
N1 - Funding Information:
ACKHOWLKDGKMEBTB: This work was supported by Rational Institutes of Health Grant AI18758 (to GJ.T.), by grants froa the Rational Institutes of Health, American Cancer Society and Rational Aeronautics and Space Administration (to A. R.), and by The Netherlands Organisation for the Advancement of Pure Research (ZWO) (to J. H. vB.).
PY - 1984/7/25
Y1 - 1984/7/25
N2 - Reman spectra were obtained from single crystals of [d(CGCATGCG)]2 and [d(m5CGTAm5CG)]2, both of which incorporate A-T pairs into Z-DNA structures and contain C2′-endo/syn conformers of deoxyguanosine at the oligonucleotide ends. Correlation with x-ray results permits the following Reman assignments for nucleoside conformers: C3′-endo/syn C, 623±1; C2′-endolsyn C, 671±2; C end C, 782±1; C2′endo/anti T, 650±5 and a. 750; C3′-endolsyn A, 729±1 cm . These results show that (i) the 670 cm line of syn G is highly 1 sensitive to the change from C3-endo to Cr′-endo pucker, (ii) the 729 cm-1 line of A is affected neither by furanose pucker nor glycosidic bond orienta tion and (iii) the 1200-1500 cm-1 region of the Reman spectrum of the A-T double helix is greatly altered by the B-to-Z transition. Conformation sensitive Raman frequencies in the 850-1700 cm-1 region are identified for both octamer and hexamer, and the Z-to-B transition of each is monitored by spectral changes which occur upon dissolving the crystal in H2O solution.
AB - Reman spectra were obtained from single crystals of [d(CGCATGCG)]2 and [d(m5CGTAm5CG)]2, both of which incorporate A-T pairs into Z-DNA structures and contain C2′-endo/syn conformers of deoxyguanosine at the oligonucleotide ends. Correlation with x-ray results permits the following Reman assignments for nucleoside conformers: C3′-endo/syn C, 623±1; C2′-endolsyn C, 671±2; C end C, 782±1; C2′endo/anti T, 650±5 and a. 750; C3′-endolsyn A, 729±1 cm . These results show that (i) the 670 cm line of syn G is highly 1 sensitive to the change from C3-endo to Cr′-endo pucker, (ii) the 729 cm-1 line of A is affected neither by furanose pucker nor glycosidic bond orienta tion and (iii) the 1200-1500 cm-1 region of the Reman spectrum of the A-T double helix is greatly altered by the B-to-Z transition. Conformation sensitive Raman frequencies in the 850-1700 cm-1 region are identified for both octamer and hexamer, and the Z-to-B transition of each is monitored by spectral changes which occur upon dissolving the crystal in H2O solution.
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U2 - 10.1093/nar/12.14.5913
DO - 10.1093/nar/12.14.5913
M3 - Article
C2 - 6462921
AN - SCOPUS:0021770762
SN - 0305-1048
VL - 12
SP - 5913
EP - 5925
JO - Nucleic Acids Research
JF - Nucleic Acids Research
IS - 14
ER -