The potential of a tailored biomimetic hydrogel for in vitro cell culture applications: Characterization and biocompatibility

Yung Chieh Cho, Hsiao Ting Huang, Wen Chien Lan, Mao Suan Huang, Takashi Saito, Bai Hung Huang, Chi Hsun Tsai, Fang Yu Fan, Keng Liang Ou

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)


In this study, the Pluronic F127 with modified tripeptide Gly-Arg-Gly-Asp copolymer (hereafter defined as 3BE) hydrogel was evaluated in terms of its biocompatibility potentials. The fibroblasts (Swiss 3T3 cell line) and human hair follicles-derived mesenchymal stem cells (HFMSCs) were cultured in different concentrations of the 3BE hydrogel (0%, 0.05%, 0.1%, 0.25%, and 0.5%, respectively). The cell morphology and differentiation potential of HFMSCs were observed through optical microscopy, and the cell viability was investigated via Live/Dead Kit and Cell Counting Kit-8 assay. Analytical results showed that HFMSC can differentiate into adipogenic, chondrogenic, and osteogenic lineages. The HFMSC and Swiss 3T3 cells would properly assemble into a spherical shape as cultured with the 3BE hydrogel. Most importantly, cell viability could be maintained above 70%. The formation of spheroid structures of cells within this hydrogel is predicted to promote cell differentiation potentials of HFMSC that benefit in generating functional adipocytes, chondrocytes, and osteoblasts. Therefore, these findings demonstrate that the 3BE hydrogel has great potential as a three-dimensional cell culture scaffold for tissue engineering applications.

Original languageEnglish
Article number9035
Pages (from-to)1-13
Number of pages13
JournalApplied Sciences (Switzerland)
Issue number24
Publication statusPublished - Dec 2 2020


  • Hair follicle
  • Mesenchymal stem cells
  • Pluronic F127
  • Spheroids
  • Tripeptide Arg-Gly-Asp

ASJC Scopus subject areas

  • General Materials Science
  • Instrumentation
  • General Engineering
  • Process Chemistry and Technology
  • Computer Science Applications
  • Fluid Flow and Transfer Processes


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