@article{64cc83142ee84377adb66e033d4fbbc4,
title = "The microRNA-210-stathmin1 axis decreases cell stiffness to facilitate the invasiveness of colorectal cancer stem cells",
abstract = "Cell migration is critical for regional dissemination and distal metastasis of cancer cells, which remain the major causes of poor prognosis and death in patients with colorectal cancer (CRC). Although cytoskeletal dynamics and cellular deformability contribute to the migration of cancer cells and metastasis, the mechanisms governing the migratory ability of cancer stem cells (CSCs), a nongenetic source of tumor heterogeneity, are unclear. Here, we expanded colorectal CSCs (CRCSCs) as colonospheres and showed that CRCSCs exhibited higher cell motility in transwell migration assays and 3D invasion assays and greater deformability in particle tracking microrheology than did their parental CRC cells. Mechanistically, in CRCSCs, microRNA-210-3p (miR-210) targeted stathmin1 (STMN1), which is known for inducing microtubule destabilization, to decrease cell elasticity in order to facilitate cell motility without affecting the epithelial–mesenchymal transition (EMT) status. Clinically, the miR-210-STMN1 axis was activated in CRC patients with liver metastasis and correlated with a worse clinical outcome. This study elucidates a miRNA-oriented mechanism regulating the deformability of CRCSCs beyond the EMT process.",
keywords = "Cancer stem cells, Colon cancer, Deformability, MicroRNAs",
author = "Liao, {Tsai Tsen} and Cheng, {Wei Chung} and Yang, {Chih Yung} and Chen, {Yin Quan} and Su, {Shu Han} and Yeh, {Tzu Yu} and Lan, {Hsin Yi} and Lee, {Chih Chan} and Lin, {Hung Hsin} and Lin, {Chun Chi} and Lu, {Ruey Hwa} and Chiou, {Arthur Er Terg} and Jiang, {Jeng Kai} and Hwang, {Wei Lun}",
note = "Funding Information: Acknowledgments: We would like to dedicate this paper to the memory of Hsei-Wei Wang (Institute of Microbiology and Immunology, National Yang-Ming University), who passed away during the period of this research. Wang initiated this study in 2014, and this paper could not have been completed without his enduring devotion to cancer genomics. We appreciate the assistance of the Biobank at Taipei Veterans General Hospital for providing human specimens. This work was supported by Taipei City Hospital, Yen Tjing Ling Medical Foundation, in Taiwan and by the Cancer Progression Research Center, National Yang-Ming University, from The Featured Areas Research Center Program within the framework of the Higher Education Sprout Project by the Ministry of Education (MOE). We thank Springer Nature Author Services for editing assistance. Funding Information: Ministry of Science and Technology (109-2636-B-038-001 and 110-2636-B-038-005 to T.-T.L.; 109-2314-B-075-033 to J.-K.J.; 107-2221-E-010-009-MY2 and 109-2221-E-010-018 to Y.-Q.C.; 108-2622-E-039-005-CC2, 109-2628-E-039-001-MY3 and MOST 109-2327-B-039-002 to W.-C.C.; 109-2320-B-010-021 to W.-L.H.). Taipei Medical University (TMU108-AE1-B25 to T.-T.L.). China Medical University (CMU107-S-24, CMU109-MF-61 and CMU109-MF-93 to W.-C.C.). Taipei Veterans General Hospital (V108C-164 to J.-K.J.). Taipei City Hospital (TPCH-109-01 to C.-Y.Y.). Yen Tjing Ling Medical Foundation (CI-109-11 to W.-L.H.). Ministry of Health and Welfare, Center of Excellence for Cancer Research (MOHW107-TDU-B-211-114019, 109 CRC-T208 and 110 CRC-T208 to W.-L.H.). We would like to dedicate this paper to the memory of Hsei-Wei Wang (Institute of Microbiology and Immunology, National Yang-Ming University), who passed away during the period of this research. Wang initiated this study in 2014, and this paper could not have been completed without his enduring devotion to cancer genomics. We appreciate the assistance of the Biobank at Taipei Veterans General Hospital for providing human specimens. This work was supported by Taipei City Hospital, Yen Tjing Ling Medical Foundation, in Taiwan and by the Cancer Progression Research Center, National Yang-Ming University, from The Featured Areas Research Center Program within the framework of the Higher Education Sprout Project by the Ministry of Education (MOE). We thank Springer Nature Author Services for editing assistance. Funding Information: Funding: Ministry of Science and Technology (109-2636-B-038-001 and 110-2636-B-038-005 to T.-T.L.; 109-2314-B-075 -033 to J.-K.J.; 107-2221-E-010-009-MY2 and 109-2221-E-010-018 to Y.-Q.C.; 108-2622-E-039-005-CC2, 109-2628-E-039-001-MY3 and MOST 109-2327-B-039-002 to W.-C.C.; 109-2320-B-010-021 to W.-L.H.). Taipei Medical University (TMU108-AE1-B25 to T.-T.L.). China Medical University (CMU107-S-24, CMU109-MF-61 and CMU109-MF-93 to W.-C.C.). Taipei Veterans General Hospital (V108C-164 to J.-K.J.). Taipei City Hospital (TPCH-109-01 to C.-Y.Y.). Yen Tjing Ling Medical Foundation (CI-109-11 to W.-L.H.). Ministry of Health and Welfare, Center of Excellence for Cancer Research (MOHW107-TDU-B-211-114019, 109 CRC-T208 and 110 CRC-T208 to W.-L.H.). Publisher Copyright: {\textcopyright} 2021 by the authors. Licensee MDPI, Basel, Switzerland.",
year = "2021",
month = apr,
day = "2",
doi = "10.3390/cancers13081833",
language = "English",
volume = "13",
journal = "Cancers",
issn = "2072-6694",
publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",
number = "8",
}
