The effects of calcium phosphate particles on the growth of osteoblasts

Jui Sheng Sun, Yang Hwei Tsuang, Chun J. Liao, Hwa Chang Liu, Yi Shiong Hang, Feng Huei Lin

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117 Citations (Scopus)


With advances in ceramics technology, calcium phosphate bioceramics have been applied as bone substitutes for several decades. The focus of this work is to elucidate the biocompatibility of the particulates of various calcium phosphate cytotoxicities. Four different kinds of calcium phosphate powders, including β-tricalcium phosphate (β-TCP), hydroxyapatite (HA), β-dicalcium pyrophosphate (β-DCP), and sintered β-dicalcium pyrophosphate (SDCP), were tested by osteoblast cell culture. The results were analyzed by cell count, concentration of transforming growth factor-β1 (TGF-β1), alkaline phosphatase (ALP), and prostaglandin E2 (PGE2) in culture media. The changes were most significant when osteoblasts were cultured with β-TCP and HA bioceramics. The changes in cell population of the β-TCP and HA were quite low in the first 3 days, then increased gradually toward the seventh day. The changes in TGF-β1 concentration in culture medium inversely related to the changes in cell population. The ALP titer in the culture media of the β-TCP and HA were quite high in the first 3 days, then decreased rapidly between the third and seventh days. The concentrations of PGE2 in the culture media tested were quite high on the first day, decreased rapidly to the third day, and then gradually until the seventh day. The changes in the β-DCP and SDCP were quite similar to those of HA and β-TCP but much less significant. We conclude that HA and β-TCP have an inhibitory effect on the growth of osteoblasts. The inhibitins effects of the HA and β-TCP powders on the osteoblast cell cultures possibly are mediated by the increased synthesis of PGE2.

Original languageEnglish
Pages (from-to)324-334
Number of pages11
JournalJournal of Biomedical Materials Research
Issue number3
Publication statusPublished - Dec 5 1997
Externally publishedYes


  • Biocompatibility
  • Calcium phosphate
  • Osteoblast
  • Particle

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials


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