TY - JOUR
T1 - The effects of calcium phosphate particles on the growth of osteoblasts
AU - Sun, Jui Sheng
AU - Tsuang, Yang Hwei
AU - Liao, Chun Jen
AU - Liu, Hwa Chang
AU - Hang, Yi Shiong
AU - Lin, Feng Huei
PY - 1997/12/5
Y1 - 1997/12/5
N2 - With advances in ceramics technology, calcium phosphate bioceramics have been applied as bone substitutes for several decades. The focus of this work is to elucidate the biocompatibility of the particulates of various calcium phosphate cytotoxicities. Four different kinds of calcium phosphate powders, including β-tricalcium phosphate (β-TCP), hydroxyapatite (HA), β-dicalcium pyrophosphate (β-DCP), and sintered β-dicalcium pyrophosphate (SDCP), were tested by osteoblast cell culture. The results were analyzed by cell count, concentration of transforming growth factor-β1 (TGF-β1), alkaline phosphatase (ALP), and prostaglandin E2 (PGE2) in culture media. The changes were most significant when osteoblasts were cultured with β-TCP and HA bioceramics. The changes in cell population of the β-TCP and HA were quite low in the first 3 days, then increased gradually toward the seventh day. The changes in TGF-β1 concentration in culture medium inversely related to the changes in cell population. The ALP titer in the culture media of the β-TCP and HA were quite high in the first 3 days, then decreased rapidly between the third and seventh days. The concentrations of PGE2 in the culture media tested were quite high on the first day, decreased rapidly to the third day, and then gradually until the seventh day. The changes in the β-DCP and SDCP were quite similar to those of HA and β-TCP but much less significant. We conclude that HA and β-TCP have an inhibitory effect on the growth of osteoblasts. The inhibitins effects of the HA and β-TCP powders on the osteoblast cell cultures possibly are mediated by the increased synthesis of PGE2.
AB - With advances in ceramics technology, calcium phosphate bioceramics have been applied as bone substitutes for several decades. The focus of this work is to elucidate the biocompatibility of the particulates of various calcium phosphate cytotoxicities. Four different kinds of calcium phosphate powders, including β-tricalcium phosphate (β-TCP), hydroxyapatite (HA), β-dicalcium pyrophosphate (β-DCP), and sintered β-dicalcium pyrophosphate (SDCP), were tested by osteoblast cell culture. The results were analyzed by cell count, concentration of transforming growth factor-β1 (TGF-β1), alkaline phosphatase (ALP), and prostaglandin E2 (PGE2) in culture media. The changes were most significant when osteoblasts were cultured with β-TCP and HA bioceramics. The changes in cell population of the β-TCP and HA were quite low in the first 3 days, then increased gradually toward the seventh day. The changes in TGF-β1 concentration in culture medium inversely related to the changes in cell population. The ALP titer in the culture media of the β-TCP and HA were quite high in the first 3 days, then decreased rapidly between the third and seventh days. The concentrations of PGE2 in the culture media tested were quite high on the first day, decreased rapidly to the third day, and then gradually until the seventh day. The changes in the β-DCP and SDCP were quite similar to those of HA and β-TCP but much less significant. We conclude that HA and β-TCP have an inhibitory effect on the growth of osteoblasts. The inhibitins effects of the HA and β-TCP powders on the osteoblast cell cultures possibly are mediated by the increased synthesis of PGE2.
KW - Biocompatibility
KW - Calcium phosphate
KW - Osteoblast
KW - Particle
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U2 - 10.1002/(SICI)1097-4636(19971205)37:3<324::AID-JBM3>3.0.CO;2-N
DO - 10.1002/(SICI)1097-4636(19971205)37:3<324::AID-JBM3>3.0.CO;2-N
M3 - Article
C2 - 9368137
AN - SCOPUS:0031555491
SN - 0021-9304
VL - 37
SP - 324
EP - 334
JO - Journal of Biomedical Materials Research
JF - Journal of Biomedical Materials Research
IS - 3
ER -