The effect of sintered β-dicalcium pyrophosphate particle size on newborn wistar rat osteoblasts

Jui Sheng Sun, Yang Hwei Tsuang, Chun Jen Liao, Hwa Chang Liu, Yi Shiong Hang, Feng Huei Lin

Research output: Contribution to journalArticlepeer-review

21 Citations (Scopus)


During recent years, sintered dicalcium phosphate (SDCP) has been shown to be an effective artificial bone filler for repairing bone defects. The goal of this study was to elucidate the effect of SDCP particle size on osteoblasts. Osteoblasts were mixed and cultured with various sized SDCP particles (0.5-3.0, 37-63, 177-250, and 420-841 μm) for 1 h, 3 h, 1 day, 3 days, and 7 days and then analyzed. The results show that the adding of smaller sized SDCP particles (0.5-3.0 and 37-63 μm) into osteoblast culture can significantly affect the cell counts of osteoblasts. The secretion of transforming growth factor-β1, alkaline phosphatase, and prostaglandin E2 in culture medium increased significantly. The changes were most significant and persisted longer in smaller particle groups. Small sintered dicalcium phosphate particles can inhibit the proliferation of the osteoblasts. The inhibitory effects of the smaller sized SDCP particles on the osteoblasts were mediated by the promotion of osteoblast differentiation and the increased synthesis of prostaglandin E2.

Original languageEnglish
Pages (from-to)331-338
Number of pages8
JournalArtificial Organs
Issue number4
Publication statusPublished - 1999
Externally publishedYes


  • Osteoblasts
  • Particle size
  • Prostaglandin E
  • Sintered dicalcium phosphate
  • Transforming growth factor

ASJC Scopus subject areas

  • Bioengineering
  • Medicine (miscellaneous)
  • Biomaterials
  • Biomedical Engineering


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