TY - JOUR
T1 - The effect of Dolichandrone serrulata (wall. ex DC.) Seem. flower extract containing antioxidant capacity and terpenoids on the male reproductive system
AU - Chaimontri, Chadaporn
AU - Arun, Supatcharee
AU - Sawatpanich, Tarinee
AU - Yannasithinon, Supataechasit
AU - Tangsrisakda, Nareelak
AU - Bunsueb, Sudtida
AU - Wu, Alexander Tsang Hsien
AU - Iamsaard, Sitthichai
N1 - Funding Information:
This study was granted by Faculty of Medicine, Khon Kaen University, Thailand (Grant Number IN63235), and we also would like to thank Human High Performance and Health Promotion, Khon Kaen University for their grant funding to associate professor Dr. Sitthichai Iamsaard. We would also like to thank Dr. Justin Thomas Reese, for editing the manuscript via Publication Clinic KKU, Thailand.
Publisher Copyright:
© 2021 Wiley-VCH GmbH
PY - 2021/4
Y1 - 2021/4
N2 - Although the fruit extract of Dolichandrone genus was shown to inhibit spermatogenesis, the reproductive toxicity of Dolichandrone serrulata flowers (DSFs) is not documented. Recent study aimed to evaluate the sub-chronic toxicity of DSF on male reproductive system. Antioxidant capacity and total phenolic contents of DSF extract were determined using Folin–Ciocalteu's, 2,2-diphenyl-1-picrylhydrazyl and ferric reducing antioxidant power assays. The terpenoid components were determined using nuclear magnetic resonance spectrum. Adult male rats were treated orally with DSF (100, 300 or 600 mg/kg) for 48 days. Histopathology of testis and epididymis was observed. Sperm concentration, viability, acrosome status and morphology were also examined. Expressions of heat shock protein 70 (Hsp70), tyrosine-phosphorylated (TyrPho) proteins, androgen receptor (AR) and steroidogenic acute regulatory (StAR) protein in testis were investigated. Results showed that DSF contained phenolic compounds and terpenoids (phytoandrogens; rengyolone and cleroindicin B). No reproductive histopathology was observed in DSF-treated rats. Although DSF decreased the serum testosterone level, the sperm qualities were not affected. Particularly, sperm concentration of DSF-treated animals was significantly increased. DSF changed the testicular TyrPho proteins but the expression of AR, StAR or Hsp70 was not altered. In conclusion, DSF possesses antioxidant capacity with no toxicity on male reproductive system.
AB - Although the fruit extract of Dolichandrone genus was shown to inhibit spermatogenesis, the reproductive toxicity of Dolichandrone serrulata flowers (DSFs) is not documented. Recent study aimed to evaluate the sub-chronic toxicity of DSF on male reproductive system. Antioxidant capacity and total phenolic contents of DSF extract were determined using Folin–Ciocalteu's, 2,2-diphenyl-1-picrylhydrazyl and ferric reducing antioxidant power assays. The terpenoid components were determined using nuclear magnetic resonance spectrum. Adult male rats were treated orally with DSF (100, 300 or 600 mg/kg) for 48 days. Histopathology of testis and epididymis was observed. Sperm concentration, viability, acrosome status and morphology were also examined. Expressions of heat shock protein 70 (Hsp70), tyrosine-phosphorylated (TyrPho) proteins, androgen receptor (AR) and steroidogenic acute regulatory (StAR) protein in testis were investigated. Results showed that DSF contained phenolic compounds and terpenoids (phytoandrogens; rengyolone and cleroindicin B). No reproductive histopathology was observed in DSF-treated rats. Although DSF decreased the serum testosterone level, the sperm qualities were not affected. Particularly, sperm concentration of DSF-treated animals was significantly increased. DSF changed the testicular TyrPho proteins but the expression of AR, StAR or Hsp70 was not altered. In conclusion, DSF possesses antioxidant capacity with no toxicity on male reproductive system.
KW - Dolichandrone serrulata (Wall. ex DC.) Seem
KW - rats
KW - reproductive system
KW - sub-chronic toxicity
KW - terpenoids
UR - http://www.scopus.com/inward/record.url?scp=85099086604&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85099086604&partnerID=8YFLogxK
U2 - 10.1111/and.13966
DO - 10.1111/and.13966
M3 - Article
C2 - 33427326
AN - SCOPUS:85099086604
SN - 0303-4569
VL - 53
JO - Andrologia
JF - Andrologia
IS - 3
M1 - e13966
ER -