The effect of copper ion on arachidonic acid metabolism in the porcine corneal epithelium

Ming T. Lin; Yui Hsi Wang; Yuh Ling Chen; Wen Chang Chang

doi:10.1006/bbrc.1993.1166

The effect of copper ion on arachidonic acid metabolism in the porcine corneal epithelium

Ming T. Lin, Yui Hsi Wang, Yuh Ling Chen, Wen Chang Chang

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

Abstract

The effect of copper ion on arachidonic acid metabolism in the corneal epithelium was studied. Our results showed that when ³H-arachidonic acid (1 μCi/μg) was incubated with homogenate of corneal epithelium at 37°C for 45 min., three metabolites were formed. Metabolite A, the major metabolite, was identified as 12-HETE by HPLC and GC. An analysis with chiral phase HPLC revealed that metabolite A was a (R)enantiomer. The production of 12(R)-HETE was stimulated by low concentrations of CuCl₂ (< 0.1 mM) and inhibited by higher concentrations of CuCl₂. Study on the biological activity of 12(R)-HETE showed that it could stimulate migration of endothelial cells in a concentration as low as 10 ng/ml. These results suggest that one of the effects of copper ion on the corneal neovascularization may be to regulate the level of 12(R)-HETE.

Original language	English
Pages (from-to)	1122-1129
Number of pages	8
Journal	Biochemical and Biophysical Research Communications
Volume	190
Issue number	3
DOIs	https://doi.org/10.1006/bbrc.1993.1166
Publication status	Published - Feb 15 1993
Externally published	Yes

ASJC Scopus subject areas

Molecular Biology
Biophysics
Biochemistry
Cell Biology

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title = "The effect of copper ion on arachidonic acid metabolism in the porcine corneal epithelium",

abstract = "The effect of copper ion on arachidonic acid metabolism in the corneal epithelium was studied. Our results showed that when 3H-arachidonic acid (1 μCi/μg) was incubated with homogenate of corneal epithelium at 37°C for 45 min., three metabolites were formed. Metabolite A, the major metabolite, was identified as 12-HETE by HPLC and GC. An analysis with chiral phase HPLC revealed that metabolite A was a (R)enantiomer. The production of 12(R)-HETE was stimulated by low concentrations of CuCl2 (< 0.1 mM) and inhibited by higher concentrations of CuCl2. Study on the biological activity of 12(R)-HETE showed that it could stimulate migration of endothelial cells in a concentration as low as 10 ng/ml. These results suggest that one of the effects of copper ion on the corneal neovascularization may be to regulate the level of 12(R)-HETE.",

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AU - Lin, Ming T.

AU - Wang, Yui Hsi

AU - Chen, Yuh Ling

AU - Chang, Wen Chang

PY - 1993/2/15

Y1 - 1993/2/15

N2 - The effect of copper ion on arachidonic acid metabolism in the corneal epithelium was studied. Our results showed that when 3H-arachidonic acid (1 μCi/μg) was incubated with homogenate of corneal epithelium at 37°C for 45 min., three metabolites were formed. Metabolite A, the major metabolite, was identified as 12-HETE by HPLC and GC. An analysis with chiral phase HPLC revealed that metabolite A was a (R)enantiomer. The production of 12(R)-HETE was stimulated by low concentrations of CuCl2 (< 0.1 mM) and inhibited by higher concentrations of CuCl2. Study on the biological activity of 12(R)-HETE showed that it could stimulate migration of endothelial cells in a concentration as low as 10 ng/ml. These results suggest that one of the effects of copper ion on the corneal neovascularization may be to regulate the level of 12(R)-HETE.

AB - The effect of copper ion on arachidonic acid metabolism in the corneal epithelium was studied. Our results showed that when 3H-arachidonic acid (1 μCi/μg) was incubated with homogenate of corneal epithelium at 37°C for 45 min., three metabolites were formed. Metabolite A, the major metabolite, was identified as 12-HETE by HPLC and GC. An analysis with chiral phase HPLC revealed that metabolite A was a (R)enantiomer. The production of 12(R)-HETE was stimulated by low concentrations of CuCl2 (< 0.1 mM) and inhibited by higher concentrations of CuCl2. Study on the biological activity of 12(R)-HETE showed that it could stimulate migration of endothelial cells in a concentration as low as 10 ng/ml. These results suggest that one of the effects of copper ion on the corneal neovascularization may be to regulate the level of 12(R)-HETE.

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The effect of copper ion on arachidonic acid metabolism in the porcine corneal epithelium

Abstract

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