The crystal structure of d(Ccccgggg): A new a-form variant with an extended backbone conformation

T. E. Haran, Z. Shakked, A. H. Wang, A. Rich

Research output: Contribution to journalArticlepeer-review

100 Citations (Scopus)


The crystal structure of d(CCCCGGGG) has been determined at a resolution of 2.25Ȧ. The oligomers crystallize as A-DNA duplexes occupying crystallographic two-fold axes. The backbone conformation is, in general, similar to that observed in previously reported crystal structures of A-DNA fragments, except for the central linkage, where it adopts an extended structure resulting from all trans conformation at the P-05’-C5’-C4′ bonds. This type of conformation facilitates interstrand stacking between the guanines at the C-G site. The local helix twist at this step is very small (25°) compared to an overall average of 33.5°. The unique structure of the C-G base-pair step, namely the extended backbone and the distinct stacking geometry, may be an important feature in the recognition mechanism between doublestranded DNA molecules and restriction endonucleases such as Msp I, which cuts the sequence CCGG very specifically with a rate unaffected by neighboring base pairs.

Original languageEnglish
Pages (from-to)199-218
Number of pages20
JournalJournal of Biomolecular Structure and Dynamics
Issue number2
Publication statusPublished - Oct 1987
Externally publishedYes

ASJC Scopus subject areas

  • Structural Biology
  • Molecular Biology


Dive into the research topics of 'The crystal structure of d(Ccccgggg): A new a-form variant with an extended backbone conformation'. Together they form a unique fingerprint.

Cite this