The complete nucleotide sequence of the growth-hormone gene from the common carp (Cyprinus carpio)

Chien Shun Chiou; Huang Tsu Chen; Wen Chang Chang

doi:10.1016/0167-4781(90)90126-M

The complete nucleotide sequence of the growth-hormone gene from the common carp (Cyprinus carpio)

Chien Shun Chiou, Huang Tsu Chen, Wen Chang Chang

Research output: Contribution to journal › Article › peer-review

60 Citations (Scopus)

Abstract

We have isolated and sequenced a phage clone from a common carp (Cyprinus carpio) genomic library that carries a gene encoding growth hormone (GH). This gene consists of five exons and four introns spanning a region of about 3 kilobasepairs. Its exons correspond with one of two reported cDNAs of carp GH except for nine differences in the nucleotide sequence, while the encoded amino-acid sequences are identical. The sequence upstream from the transcription start point contains two tandem repeats of AACTCTCATG (from -85 to -62) and the typical TATA box. All the introns start with a consensus GT dinucleotide and end with AG. The arrangement of exons and introns is very similar to that seen in mammalian GH, but quite different from the GH genes of rainbow trout and Atlantic salmon.

Original language	English
Pages (from-to)	91-94
Number of pages	4
Journal	BBA - Gene Structure and Expression
Volume	1087
Issue number	1
DOIs	https://doi.org/10.1016/0167-4781(90)90126-M
Publication status	Published - Sept 10 1990
Externally published	Yes

Keywords

(C. carpio)
(Carp)
Exon
Genomic sequence
Growth hormone
Intron

ASJC Scopus subject areas

Structural Biology
Biophysics
Biochemistry
Genetics

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10.1016/0167-4781(90)90126-M

Cite this

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title = "The complete nucleotide sequence of the growth-hormone gene from the common carp (Cyprinus carpio)",

abstract = "We have isolated and sequenced a phage clone from a common carp (Cyprinus carpio) genomic library that carries a gene encoding growth hormone (GH). This gene consists of five exons and four introns spanning a region of about 3 kilobasepairs. Its exons correspond with one of two reported cDNAs of carp GH except for nine differences in the nucleotide sequence, while the encoded amino-acid sequences are identical. The sequence upstream from the transcription start point contains two tandem repeats of AACTCTCATG (from -85 to -62) and the typical TATA box. All the introns start with a consensus GT dinucleotide and end with AG. The arrangement of exons and introns is very similar to that seen in mammalian GH, but quite different from the GH genes of rainbow trout and Atlantic salmon.",

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AU - Chiou, Chien Shun

AU - Chen, Huang Tsu

AU - Chang, Wen Chang

PY - 1990/9/10

Y1 - 1990/9/10

N2 - We have isolated and sequenced a phage clone from a common carp (Cyprinus carpio) genomic library that carries a gene encoding growth hormone (GH). This gene consists of five exons and four introns spanning a region of about 3 kilobasepairs. Its exons correspond with one of two reported cDNAs of carp GH except for nine differences in the nucleotide sequence, while the encoded amino-acid sequences are identical. The sequence upstream from the transcription start point contains two tandem repeats of AACTCTCATG (from -85 to -62) and the typical TATA box. All the introns start with a consensus GT dinucleotide and end with AG. The arrangement of exons and introns is very similar to that seen in mammalian GH, but quite different from the GH genes of rainbow trout and Atlantic salmon.

AB - We have isolated and sequenced a phage clone from a common carp (Cyprinus carpio) genomic library that carries a gene encoding growth hormone (GH). This gene consists of five exons and four introns spanning a region of about 3 kilobasepairs. Its exons correspond with one of two reported cDNAs of carp GH except for nine differences in the nucleotide sequence, while the encoded amino-acid sequences are identical. The sequence upstream from the transcription start point contains two tandem repeats of AACTCTCATG (from -85 to -62) and the typical TATA box. All the introns start with a consensus GT dinucleotide and end with AG. The arrangement of exons and introns is very similar to that seen in mammalian GH, but quite different from the GH genes of rainbow trout and Atlantic salmon.

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KW - Exon

KW - Genomic sequence

KW - Growth hormone

KW - Intron

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The complete nucleotide sequence of the growth-hormone gene from the common carp (Cyprinus carpio)

Abstract

Keywords

ASJC Scopus subject areas

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