The cloning, crystallization and preliminary X-ray analysis of XC2113, a YaeQ protein from Xanthomonas campestris

Kuo Cheng Chio; Fei Philip Gao; Ko Hsin Chin; Ping Chiang Lyu; Hui Lin Shr; Andrew H.J. Wang; Shan Ho Chou

doi:10.1107/S1744309106038383

The cloning, crystallization and preliminary X-ray analysis of XC2113, a YaeQ protein from Xanthomonas campestris

Kuo Cheng Chio, Fei Philip Gao, Ko Hsin Chin, Ping Chiang Lyu, Hui Lin Shr, Andrew H.J. Wang, Shan Ho Chou

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

Xanthomonas campestris is a Gram-negative bacterium that is phytopathogenic to cruciferous plants and causes worldwide agricultural loss. It is therefore important to identify potential pathogenic factors involved in this plant disease. Here, the cloning, expression, crystallization and preliminary X-ray analysis of XC2113, a YaeQ protein possibly involved in the production of virulence factors in Xanthomonas campestris pathovar campestris, are reported. The XC2113 crystals diffracted well to a resolution of at least 1.28 Å. They are orthorhombic and belong to space group P2₁2 ₁2₁, with unit-cell parameters a = 32.86, b = 62.69, c = 79.96 Å.

Original language	English
Pages (from-to)	1046-1048
Number of pages	3
Journal	Acta Crystallographica Section F: Structural Biology and Crystallization Communications
Volume	62
Issue number	10
DOIs	https://doi.org/10.1107/S1744309106038383
Publication status	Published - Oct 2006
Externally published	Yes

Keywords

Haemolysin
Structural genomics
Virulence factor
Xanthomonas campestris
YaeQ

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Genetics
Condensed Matter Physics

Access to Document

10.1107/S1744309106038383

Cite this

The cloning, crystallization and preliminary X-ray analysis of XC2113, a YaeQ protein from Xanthomonas campestris. / Chio, Kuo Cheng; Gao, Fei Philip; Chin, Ko Hsin et al.
In: Acta Crystallographica Section F: Structural Biology and Crystallization Communications, Vol. 62, No. 10, 10.2006, p. 1046-1048.

Research output: Contribution to journal › Article › peer-review

@article{8265bd7ced9a427198f825c2c9744f73,

title = "The cloning, crystallization and preliminary X-ray analysis of XC2113, a YaeQ protein from Xanthomonas campestris",

abstract = "Xanthomonas campestris is a Gram-negative bacterium that is phytopathogenic to cruciferous plants and causes worldwide agricultural loss. It is therefore important to identify potential pathogenic factors involved in this plant disease. Here, the cloning, expression, crystallization and preliminary X-ray analysis of XC2113, a YaeQ protein possibly involved in the production of virulence factors in Xanthomonas campestris pathovar campestris, are reported. The XC2113 crystals diffracted well to a resolution of at least 1.28 {\AA}. They are orthorhombic and belong to space group P212 121, with unit-cell parameters a = 32.86, b = 62.69, c = 79.96 {\AA}.",

keywords = "Haemolysin, Structural genomics, Virulence factor, Xanthomonas campestris, YaeQ",

author = "Chio, {Kuo Cheng} and Gao, {Fei Philip} and Chin, {Ko Hsin} and Lyu, {Ping Chiang} and Shr, {Hui Lin} and Wang, {Andrew H.J.} and Chou, {Shan Ho}",

year = "2006",

month = oct,

doi = "10.1107/S1744309106038383",

language = "English",

volume = "62",

pages = "1046--1048",

journal = "Acta Crystallographica Section F: Structural Biology and Crystallization Communications",

issn = "1744-3091",

publisher = "John Wiley and Sons Ltd",

number = "10",

}

TY - JOUR

T1 - The cloning, crystallization and preliminary X-ray analysis of XC2113, a YaeQ protein from Xanthomonas campestris

AU - Chio, Kuo Cheng

AU - Gao, Fei Philip

AU - Chin, Ko Hsin

AU - Lyu, Ping Chiang

AU - Shr, Hui Lin

AU - Wang, Andrew H.J.

AU - Chou, Shan Ho

PY - 2006/10

Y1 - 2006/10

N2 - Xanthomonas campestris is a Gram-negative bacterium that is phytopathogenic to cruciferous plants and causes worldwide agricultural loss. It is therefore important to identify potential pathogenic factors involved in this plant disease. Here, the cloning, expression, crystallization and preliminary X-ray analysis of XC2113, a YaeQ protein possibly involved in the production of virulence factors in Xanthomonas campestris pathovar campestris, are reported. The XC2113 crystals diffracted well to a resolution of at least 1.28 Å. They are orthorhombic and belong to space group P212 121, with unit-cell parameters a = 32.86, b = 62.69, c = 79.96 Å.

AB - Xanthomonas campestris is a Gram-negative bacterium that is phytopathogenic to cruciferous plants and causes worldwide agricultural loss. It is therefore important to identify potential pathogenic factors involved in this plant disease. Here, the cloning, expression, crystallization and preliminary X-ray analysis of XC2113, a YaeQ protein possibly involved in the production of virulence factors in Xanthomonas campestris pathovar campestris, are reported. The XC2113 crystals diffracted well to a resolution of at least 1.28 Å. They are orthorhombic and belong to space group P212 121, with unit-cell parameters a = 32.86, b = 62.69, c = 79.96 Å.

KW - Haemolysin

KW - Structural genomics

KW - Virulence factor

KW - Xanthomonas campestris

KW - YaeQ

UR - http://www.scopus.com/inward/record.url?scp=33749525138&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33749525138&partnerID=8YFLogxK

U2 - 10.1107/S1744309106038383

DO - 10.1107/S1744309106038383

M3 - Article

C2 - 17012809

AN - SCOPUS:33749525138

SN - 1744-3091

VL - 62

SP - 1046

EP - 1048

JO - Acta Crystallographica Section F: Structural Biology and Crystallization Communications

JF - Acta Crystallographica Section F: Structural Biology and Crystallization Communications

IS - 10

ER -

The cloning, crystallization and preliminary X-ray analysis of XC2113, a YaeQ protein from Xanthomonas campestris

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this