The antiproliferative effect of C2-ceramide on lung cancer cells through apoptosis by inhibiting Akt and NFκB

I. Ling Lin; Han Lin Chou; Jin Ching Lee; Feng Wei Chen; Yao Fong; Wei Chiao Chang; Hurng W. Huang; Chang Yi Wu; Wen Tsan Chang; Hui Min D. Wang; Chien Chih Chiu

doi:10.1186/1475-2867-14-1

The antiproliferative effect of C₂-ceramide on lung cancer cells through apoptosis by inhibiting Akt and NFκB

I. Ling Lin, Han Lin Chou, Jin Ching Lee, Feng Wei Chen, Yao Fong, Wei Chiao Chang, Hurng W. Huang, Chang Yi Wu, Wen Tsan Chang, Hui Min D. Wang, Chien Chih Chiu

Research output: Contribution to journal › Article › peer-review

21 Citations (Scopus)

Abstract

The anticancer effects of ceramide have been reported in many types of cancers but less in lung cancer. In this study, we used C₂-ceramide to further investigate its possible anticancer effects and mechanisms on non-small cell lung cancer (NSCLC) H1299 cells. The result of cell proliferation in terms of trypan blue assay showed high dose of C₂-ceramide inhibited cell survival after 24 h treatment. The flow cytometry-based assays indicated the effect of apoptosis, chromatin condensation, and G₁ arrest in terms of Annexin V/propidium iodide (PI), DAPI, and PI stainings, respectively. Moreover, the decreased protein level of p-Akt, p-NFκB, survivin and cyclin A2 were detected by Western blot assay. Taken together, these results indicated the antiproliferative effect of C₂-ceramide is majorly responsible for cell apoptosis in lung cancer H1299 cells.

Original language	English
Article number	1
Journal	Cancer Cell International
Volume	14
Issue number	1
DOIs	https://doi.org/10.1186/1475-2867-14-1
Publication status	Published - Jan 6 2014

Keywords

Apoptosis
Ceramides
Lung cancer
NSCLC
cyclin A2
p-Akt
p-NFκB
survivin

ASJC Scopus subject areas

Oncology
Genetics
Cancer Research

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1186/1475-2867-14-1

Cite this

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title = "The antiproliferative effect of C2-ceramide on lung cancer cells through apoptosis by inhibiting Akt and NFκB",

abstract = "The anticancer effects of ceramide have been reported in many types of cancers but less in lung cancer. In this study, we used C2-ceramide to further investigate its possible anticancer effects and mechanisms on non-small cell lung cancer (NSCLC) H1299 cells. The result of cell proliferation in terms of trypan blue assay showed high dose of C2-ceramide inhibited cell survival after 24 h treatment. The flow cytometry-based assays indicated the effect of apoptosis, chromatin condensation, and G1 arrest in terms of Annexin V/propidium iodide (PI), DAPI, and PI stainings, respectively. Moreover, the decreased protein level of p-Akt, p-NFκB, survivin and cyclin A2 were detected by Western blot assay. Taken together, these results indicated the antiproliferative effect of C2-ceramide is majorly responsible for cell apoptosis in lung cancer H1299 cells.",

keywords = "Apoptosis, Ceramides, Lung cancer, NSCLC, cyclin A2, p-Akt, p-NFκB, survivin",

author = "Lin, {I. Ling} and Chou, {Han Lin} and Lee, {Jin Ching} and Chen, {Feng Wei} and Yao Fong and Chang, {Wei Chiao} and Huang, {Hurng W.} and Wu, {Chang Yi} and Chang, {Wen Tsan} and Wang, {Hui Min D.} and Chiu, {Chien Chih}",

note = "Funding Information: This study was financially supported by grants NSC101-2313-B-037-001, NSC101-2622-B-037-002-CC3 and NSC101-2320-B-037-046-MY3 from the National Science Council, Taiwan; by grant 101-CM-KMU-11 from ChiMei-KMU Joint Research Project; and by grant #NSYSUKMU 102–28 from the NSYSU-KMU Joint Research Project. The authors thank the help from the Statistical Analysis Laboratory, Department of Medical Research, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Taiwan.",

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T1 - The antiproliferative effect of C2-ceramide on lung cancer cells through apoptosis by inhibiting Akt and NFκB

AU - Lin, I. Ling

AU - Chou, Han Lin

AU - Lee, Jin Ching

AU - Chen, Feng Wei

AU - Fong, Yao

AU - Chang, Wei Chiao

AU - Huang, Hurng W.

AU - Wu, Chang Yi

AU - Chang, Wen Tsan

AU - Wang, Hui Min D.

AU - Chiu, Chien Chih

N1 - Funding Information: This study was financially supported by grants NSC101-2313-B-037-001, NSC101-2622-B-037-002-CC3 and NSC101-2320-B-037-046-MY3 from the National Science Council, Taiwan; by grant 101-CM-KMU-11 from ChiMei-KMU Joint Research Project; and by grant #NSYSUKMU 102–28 from the NSYSU-KMU Joint Research Project. The authors thank the help from the Statistical Analysis Laboratory, Department of Medical Research, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Taiwan.

PY - 2014/1/6

Y1 - 2014/1/6

N2 - The anticancer effects of ceramide have been reported in many types of cancers but less in lung cancer. In this study, we used C2-ceramide to further investigate its possible anticancer effects and mechanisms on non-small cell lung cancer (NSCLC) H1299 cells. The result of cell proliferation in terms of trypan blue assay showed high dose of C2-ceramide inhibited cell survival after 24 h treatment. The flow cytometry-based assays indicated the effect of apoptosis, chromatin condensation, and G1 arrest in terms of Annexin V/propidium iodide (PI), DAPI, and PI stainings, respectively. Moreover, the decreased protein level of p-Akt, p-NFκB, survivin and cyclin A2 were detected by Western blot assay. Taken together, these results indicated the antiproliferative effect of C2-ceramide is majorly responsible for cell apoptosis in lung cancer H1299 cells.

AB - The anticancer effects of ceramide have been reported in many types of cancers but less in lung cancer. In this study, we used C2-ceramide to further investigate its possible anticancer effects and mechanisms on non-small cell lung cancer (NSCLC) H1299 cells. The result of cell proliferation in terms of trypan blue assay showed high dose of C2-ceramide inhibited cell survival after 24 h treatment. The flow cytometry-based assays indicated the effect of apoptosis, chromatin condensation, and G1 arrest in terms of Annexin V/propidium iodide (PI), DAPI, and PI stainings, respectively. Moreover, the decreased protein level of p-Akt, p-NFκB, survivin and cyclin A2 were detected by Western blot assay. Taken together, these results indicated the antiproliferative effect of C2-ceramide is majorly responsible for cell apoptosis in lung cancer H1299 cells.

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