The antiplatelet activity of tetramethylpyrazine is mediated through activation of NO synthase

Joen Rong Sheu; Ya Chen Kan; Wei Chun Hung; Chien Huang Lin; Mao Hsiung Yen

doi:10.1016/S0024-3205(00)00686-X

The antiplatelet activity of tetramethylpyrazine is mediated through activation of NO synthase

Joen Rong Sheu, Ya Chen Kan, Wei Chun Hung, Chien Huang Lin, Mao Hsiung Yen

Research output: Contribution to journal › Article › peer-review

66 Citations (Scopus)

Abstract

Tetramethylpyrazine (TMPZ) is an active ingredient of a Chinese herbal medicine (Ligusticum wallichii Franchat). In this study, TMPZ (50-200 μM) significantly increased production of nitrate and cyclic GMP in human platelets within a 15-min incubation period. TMPZ concentration-dependently inhibited intracellular Ca²⁺ mobilization in human platelets stimulated by collagen (5 μg/ml). Furthermore, TMPZ concentration (50 and 200 μM)- and time (15 and 30 min)-dependently triggered endothelial-type constitutive nitric oxide synthase (ecNOS) protein expression in human platelets. These results indicated that TMPZ at micromolar concentrations stimulated nitric oxide production in human platelets via a novel mechanism that activated ecNOS protein expression. (C) 2000 Elsevier Science Inc.

Original language	English
Pages (from-to)	937-947
Number of pages	11
Journal	Life Sciences
Volume	67
Issue number	8
DOIs	https://doi.org/10.1016/S0024-3205(00)00686-X
Publication status	Published - Jul 14 2000

Keywords

Cyclic GMP
Nitric oxide
Platelet
Tetramethylpyrazine
ecNOS

ASJC Scopus subject areas

General Pharmacology, Toxicology and Pharmaceutics
General Biochemistry,Genetics and Molecular Biology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/S0024-3205(00)00686-X

Cite this

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title = "The antiplatelet activity of tetramethylpyrazine is mediated through activation of NO synthase",

abstract = "Tetramethylpyrazine (TMPZ) is an active ingredient of a Chinese herbal medicine (Ligusticum wallichii Franchat). In this study, TMPZ (50-200 μM) significantly increased production of nitrate and cyclic GMP in human platelets within a 15-min incubation period. TMPZ concentration-dependently inhibited intracellular Ca2+ mobilization in human platelets stimulated by collagen (5 μg/ml). Furthermore, TMPZ concentration (50 and 200 μM)- and time (15 and 30 min)-dependently triggered endothelial-type constitutive nitric oxide synthase (ecNOS) protein expression in human platelets. These results indicated that TMPZ at micromolar concentrations stimulated nitric oxide production in human platelets via a novel mechanism that activated ecNOS protein expression. (C) 2000 Elsevier Science Inc.",

keywords = "Cyclic GMP, Nitric oxide, Platelet, Tetramethylpyrazine, ecNOS",

author = "Sheu, {Joen Rong} and Kan, {Ya Chen} and Hung, {Wei Chun} and Lin, {Chien Huang} and Yen, {Mao Hsiung}",

year = "2000",

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T1 - The antiplatelet activity of tetramethylpyrazine is mediated through activation of NO synthase

AU - Sheu, Joen Rong

AU - Kan, Ya Chen

AU - Hung, Wei Chun

AU - Lin, Chien Huang

AU - Yen, Mao Hsiung

PY - 2000/7/14

Y1 - 2000/7/14

N2 - Tetramethylpyrazine (TMPZ) is an active ingredient of a Chinese herbal medicine (Ligusticum wallichii Franchat). In this study, TMPZ (50-200 μM) significantly increased production of nitrate and cyclic GMP in human platelets within a 15-min incubation period. TMPZ concentration-dependently inhibited intracellular Ca2+ mobilization in human platelets stimulated by collagen (5 μg/ml). Furthermore, TMPZ concentration (50 and 200 μM)- and time (15 and 30 min)-dependently triggered endothelial-type constitutive nitric oxide synthase (ecNOS) protein expression in human platelets. These results indicated that TMPZ at micromolar concentrations stimulated nitric oxide production in human platelets via a novel mechanism that activated ecNOS protein expression. (C) 2000 Elsevier Science Inc.

AB - Tetramethylpyrazine (TMPZ) is an active ingredient of a Chinese herbal medicine (Ligusticum wallichii Franchat). In this study, TMPZ (50-200 μM) significantly increased production of nitrate and cyclic GMP in human platelets within a 15-min incubation period. TMPZ concentration-dependently inhibited intracellular Ca2+ mobilization in human platelets stimulated by collagen (5 μg/ml). Furthermore, TMPZ concentration (50 and 200 μM)- and time (15 and 30 min)-dependently triggered endothelial-type constitutive nitric oxide synthase (ecNOS) protein expression in human platelets. These results indicated that TMPZ at micromolar concentrations stimulated nitric oxide production in human platelets via a novel mechanism that activated ecNOS protein expression. (C) 2000 Elsevier Science Inc.

KW - Cyclic GMP

KW - Nitric oxide

KW - Platelet

KW - Tetramethylpyrazine

KW - ecNOS

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DO - 10.1016/S0024-3205(00)00686-X

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JO - Life Sciences

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ER -

The antiplatelet activity of tetramethylpyrazine is mediated through activation of NO synthase

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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