The activation of directional stem cell motility by green light-emitting diode irradiation

Wei Kee Ong; How Foo Chen; Cheng Ting Tsai; Yun Ju Fu; Yi Shan Wong; Da Jen Yen; Tzu Hao Chang; Hsien Da Huang; Oscar Kuang-Sheng Lee; Shu Chien; Jennifer Hui-Chun Ho

doi:10.1016/j.biomaterials.2012.11.065

The activation of directional stem cell motility by green light-emitting diode irradiation

Wei Kee Ong, How Foo Chen, Cheng Ting Tsai, Yun Ju Fu, Yi Shan Wong, Da Jen Yen, Tzu Hao Chang, Hsien Da Huang, Oscar Kuang-Sheng Lee, Shu Chien, Jennifer Hui-Chun Ho

Research output: Contribution to journal › Article › peer-review

43 Citations (Scopus)

Abstract

Light-emitting diode (LED) irradiation is potentially a photostimulator to manipulate cell behavior by opsin-triggered phototransduction and thermal energy supply in living cells. Directional stem cell motility is critical for the efficiency and specificity of stem cells in tissue repair. We explored that green LED (530 nm) irradiation directed the human orbital fat stem cells (OFSCs) to migrate away from the LED light source through activation of extracellular signal-regulated kinases (ERK)/MAP kinase/p38 signaling pathway. ERK inhibitor selectively abrogated light-driven OFSC migration. Phosphorylation of these kinases as well as green LED irradiation-induced cell migration was facilitated by increasing adenosine triphosphate (ATP) production in OFSCs after green LED exposure, and which was thermal stress-independent mechanism. OFSCs, which are multi-potent mesenchymal stem cells isolated from human orbital fat tissue, constitutionally express three opsins, i.e. retinal pigment epithelium-derived rhodopsin homolog (RRH), encephalopsin (OPN3) and short-wave-sensitive opsin 1 (OPN1SW). However, only two non-visual opsins, i.e. RRH and OPN3, served as photoreceptors response to green LED irradiation-induced OFSC migration. In conclusion, stem cells are sensitive to green LED irradiation-induced directional cell migration through activation of ERK signaling pathway via a wavelength-dependent phototransduction.

Original language	English
Pages (from-to)	1911-1920
Number of pages	10
Journal	Biomaterials
Volume	34
Issue number	8
DOIs	https://doi.org/10.1016/j.biomaterials.2012.11.065
Publication status	Published - Mar 2013

Keywords

Cell motility
Light-emitting diode
Photosensitization
Stem cells
Wavelength

ASJC Scopus subject areas

Mechanics of Materials
Ceramics and Composites
Bioengineering
Biophysics
Biomaterials

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10.1016/j.biomaterials.2012.11.065

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title = "The activation of directional stem cell motility by green light-emitting diode irradiation",

abstract = "Light-emitting diode (LED) irradiation is potentially a photostimulator to manipulate cell behavior by opsin-triggered phototransduction and thermal energy supply in living cells. Directional stem cell motility is critical for the efficiency and specificity of stem cells in tissue repair. We explored that green LED (530 nm) irradiation directed the human orbital fat stem cells (OFSCs) to migrate away from the LED light source through activation of extracellular signal-regulated kinases (ERK)/MAP kinase/p38 signaling pathway. ERK inhibitor selectively abrogated light-driven OFSC migration. Phosphorylation of these kinases as well as green LED irradiation-induced cell migration was facilitated by increasing adenosine triphosphate (ATP) production in OFSCs after green LED exposure, and which was thermal stress-independent mechanism. OFSCs, which are multi-potent mesenchymal stem cells isolated from human orbital fat tissue, constitutionally express three opsins, i.e. retinal pigment epithelium-derived rhodopsin homolog (RRH), encephalopsin (OPN3) and short-wave-sensitive opsin 1 (OPN1SW). However, only two non-visual opsins, i.e. RRH and OPN3, served as photoreceptors response to green LED irradiation-induced OFSC migration. In conclusion, stem cells are sensitive to green LED irradiation-induced directional cell migration through activation of ERK signaling pathway via a wavelength-dependent phototransduction.",

keywords = "Cell motility, Light-emitting diode, Photosensitization, Stem cells, Wavelength",

author = "Ong, {Wei Kee} and Chen, {How Foo} and Tsai, {Cheng Ting} and Fu, {Yun Ju} and Wong, {Yi Shan} and Yen, {Da Jen} and Chang, {Tzu Hao} and Huang, {Hsien Da} and {Kuang-Sheng Lee}, Oscar and Shu Chien and {Hui-Chun Ho}, Jennifer",

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AU - Ong, Wei Kee

AU - Chen, How Foo

AU - Tsai, Cheng Ting

AU - Fu, Yun Ju

AU - Wong, Yi Shan

AU - Yen, Da Jen

AU - Chang, Tzu Hao

AU - Huang, Hsien Da

AU - Kuang-Sheng Lee, Oscar

AU - Chien, Shu

AU - Hui-Chun Ho, Jennifer

PY - 2013/3

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N2 - Light-emitting diode (LED) irradiation is potentially a photostimulator to manipulate cell behavior by opsin-triggered phototransduction and thermal energy supply in living cells. Directional stem cell motility is critical for the efficiency and specificity of stem cells in tissue repair. We explored that green LED (530 nm) irradiation directed the human orbital fat stem cells (OFSCs) to migrate away from the LED light source through activation of extracellular signal-regulated kinases (ERK)/MAP kinase/p38 signaling pathway. ERK inhibitor selectively abrogated light-driven OFSC migration. Phosphorylation of these kinases as well as green LED irradiation-induced cell migration was facilitated by increasing adenosine triphosphate (ATP) production in OFSCs after green LED exposure, and which was thermal stress-independent mechanism. OFSCs, which are multi-potent mesenchymal stem cells isolated from human orbital fat tissue, constitutionally express three opsins, i.e. retinal pigment epithelium-derived rhodopsin homolog (RRH), encephalopsin (OPN3) and short-wave-sensitive opsin 1 (OPN1SW). However, only two non-visual opsins, i.e. RRH and OPN3, served as photoreceptors response to green LED irradiation-induced OFSC migration. In conclusion, stem cells are sensitive to green LED irradiation-induced directional cell migration through activation of ERK signaling pathway via a wavelength-dependent phototransduction.

AB - Light-emitting diode (LED) irradiation is potentially a photostimulator to manipulate cell behavior by opsin-triggered phototransduction and thermal energy supply in living cells. Directional stem cell motility is critical for the efficiency and specificity of stem cells in tissue repair. We explored that green LED (530 nm) irradiation directed the human orbital fat stem cells (OFSCs) to migrate away from the LED light source through activation of extracellular signal-regulated kinases (ERK)/MAP kinase/p38 signaling pathway. ERK inhibitor selectively abrogated light-driven OFSC migration. Phosphorylation of these kinases as well as green LED irradiation-induced cell migration was facilitated by increasing adenosine triphosphate (ATP) production in OFSCs after green LED exposure, and which was thermal stress-independent mechanism. OFSCs, which are multi-potent mesenchymal stem cells isolated from human orbital fat tissue, constitutionally express three opsins, i.e. retinal pigment epithelium-derived rhodopsin homolog (RRH), encephalopsin (OPN3) and short-wave-sensitive opsin 1 (OPN1SW). However, only two non-visual opsins, i.e. RRH and OPN3, served as photoreceptors response to green LED irradiation-induced OFSC migration. In conclusion, stem cells are sensitive to green LED irradiation-induced directional cell migration through activation of ERK signaling pathway via a wavelength-dependent phototransduction.

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KW - Light-emitting diode

KW - Photosensitization

KW - Stem cells

KW - Wavelength

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The activation of directional stem cell motility by green light-emitting diode irradiation

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Keywords

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