Tetramethylpyrazine as potassium channel opener to lower calcium influx into cultured aortic smooth muscle cells

Chin Chuan Tsai; Tung-Iuan Lai; Wei Chan Huang; Tzyylin Yang; I. Min Liu; Kar Lok Wong; Paul Chan; Juei Tang Cheng

doi:10.1055/s-2003-40638

Tetramethylpyrazine as potassium channel opener to lower calcium influx into cultured aortic smooth muscle cells

Chin Chuan Tsai, Tung-Iuan Lai, Wei Chan Huang, Tzyylin Yang, I. Min Liu, Kar Lok Wong, Paul Chan, Juei Tang Cheng

Research output: Contribution to journal › Article › peer-review

13 Citations (Scopus)

Abstract

In the present study, the effect of tetramethylpyrazine (TMP) on calcium (Ca²⁺) influx was investigated in cultured vascular smooth muscle (A7r5) cells using Fura-2 as an indicator. The increase of Ca²⁺ concentration in A7r5 cells produced by vasopressin or phenylephrine was attenuated by TMP from 0.01 μmol/L to 1 mmol/L. The decrease in the intracellular potassium concentration in A7r5 cells by TMP from 0.01 μmol/L to 10 μmol/L was characterized using PBFI/AM. Inhibitors specific to the small conductance calcium-activated potassium (SK_Ca) channel or the ATP-sensitive potassium (K_ATP) channel abolished the actions of TMP. The obtained results indicate that the decrease of Ca²⁺ influx into A7r5 cells by TMP is mainly mediated by the opening of potassium channels.

Original language	English
Pages (from-to)	557-558
Number of pages	2
Journal	Planta Medica
Volume	69
Issue number	6
DOIs	https://doi.org/10.1055/s-2003-40638
Publication status	Published - Jun 1 2003

ASJC Scopus subject areas

Analytical Chemistry
Molecular Medicine
Pharmacology
Pharmaceutical Science
Drug Discovery
Complementary and alternative medicine
Organic Chemistry

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title = "Tetramethylpyrazine as potassium channel opener to lower calcium influx into cultured aortic smooth muscle cells",

abstract = "In the present study, the effect of tetramethylpyrazine (TMP) on calcium (Ca2+) influx was investigated in cultured vascular smooth muscle (A7r5) cells using Fura-2 as an indicator. The increase of Ca2+ concentration in A7r5 cells produced by vasopressin or phenylephrine was attenuated by TMP from 0.01 μmol/L to 1 mmol/L. The decrease in the intracellular potassium concentration in A7r5 cells by TMP from 0.01 μmol/L to 10 μmol/L was characterized using PBFI/AM. Inhibitors specific to the small conductance calcium-activated potassium (SKCa) channel or the ATP-sensitive potassium (KATP) channel abolished the actions of TMP. The obtained results indicate that the decrease of Ca2+ influx into A7r5 cells by TMP is mainly mediated by the opening of potassium channels.",

author = "Tsai, {Chin Chuan} and Tung-Iuan Lai and Huang, {Wei Chan} and Tzyylin Yang and Liu, {I. Min} and Wong, {Kar Lok} and Paul Chan and Cheng, {Juei Tang}",

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T1 - Tetramethylpyrazine as potassium channel opener to lower calcium influx into cultured aortic smooth muscle cells

AU - Tsai, Chin Chuan

AU - Lai, Tung-Iuan

AU - Huang, Wei Chan

AU - Yang, Tzyylin

AU - Liu, I. Min

AU - Wong, Kar Lok

AU - Chan, Paul

AU - Cheng, Juei Tang

PY - 2003/6/1

Y1 - 2003/6/1

N2 - In the present study, the effect of tetramethylpyrazine (TMP) on calcium (Ca2+) influx was investigated in cultured vascular smooth muscle (A7r5) cells using Fura-2 as an indicator. The increase of Ca2+ concentration in A7r5 cells produced by vasopressin or phenylephrine was attenuated by TMP from 0.01 μmol/L to 1 mmol/L. The decrease in the intracellular potassium concentration in A7r5 cells by TMP from 0.01 μmol/L to 10 μmol/L was characterized using PBFI/AM. Inhibitors specific to the small conductance calcium-activated potassium (SKCa) channel or the ATP-sensitive potassium (KATP) channel abolished the actions of TMP. The obtained results indicate that the decrease of Ca2+ influx into A7r5 cells by TMP is mainly mediated by the opening of potassium channels.

AB - In the present study, the effect of tetramethylpyrazine (TMP) on calcium (Ca2+) influx was investigated in cultured vascular smooth muscle (A7r5) cells using Fura-2 as an indicator. The increase of Ca2+ concentration in A7r5 cells produced by vasopressin or phenylephrine was attenuated by TMP from 0.01 μmol/L to 1 mmol/L. The decrease in the intracellular potassium concentration in A7r5 cells by TMP from 0.01 μmol/L to 10 μmol/L was characterized using PBFI/AM. Inhibitors specific to the small conductance calcium-activated potassium (SKCa) channel or the ATP-sensitive potassium (KATP) channel abolished the actions of TMP. The obtained results indicate that the decrease of Ca2+ influx into A7r5 cells by TMP is mainly mediated by the opening of potassium channels.

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JO - Planta Medica

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IS - 6

ER -

Tetramethylpyrazine as potassium channel opener to lower calcium influx into cultured aortic smooth muscle cells

Abstract

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