Terpenoids from the fermented broths of coprinellus radians

One new sesquiterpenoid, namely coprinol (1), along with guanacastanes J (2), E (3) and N (4), were isolated from the ethyl acetate extracts of the fermented broths of the fungal strain Coprinellus radians #1168. Their structures were elucidated on the basis of spectroscopic data analysis. The growth inhibitory activities against A549 of 1-4 were evaluated, and only 4 exhibited moderate growth inhibitory activity with a GI₅₀ value of 18.2 μM compared with fluorouracil (GI50 = 3.6 μM). All the compounds were also subjected to antifungal assay against Candida albicans ATCC 18804, C. albicans SC-5314, Cryptococcus neoformans ATCC 13690 and Saccharomyces cerevisiae ATCC 2345; all showed mild antifungal activity with MIC values of 128.0 μg/mL for 1-3 and 64.0 μg/mL for 4 in comparison with amphotericin B (MIC = 0.25 μg/mL) and ketoconazole (MIC = 1.0 μg/mL).