TY - JOUR
T1 - Template-free synthesis of mesoporous Ce3NbO7/CeO2 hollow nanospheres for label-free electrochemical immunosensing of leptin
AU - Liu, Xinke
AU - Tseng, Ching Li
AU - Lin, Lu Yin
AU - Lee, Chin An
AU - Li, Jian
AU - Feng, Li
AU - Song, Lijun
AU - Li, Xiaohua
AU - He, Jr Hau
AU - Sakthivel, Rajalakshmi
AU - Chung, Ren Jei
N1 - Funding Information:
The authors are grateful for the financial supports of this research by the Ministry of Science and Technology of Taiwan ( MOST 106-2221-E-027-034 ; MOST 109-2222-E-027-004 ), the National Taipei University of Technology – Shenzhen University Joint Research Program ( NTUT-SZU-110-09 (2021009) ); in part from Key-Area Research and Development Program of Guangdong Province ( 2020B010174003 ); the Science and Technology Foundation of Shenzhen ( JSGG20191129114216474 ); and the International Distinguished Visiting Professor support for Prof. Jr-Hau He from National Taipei University of Technology . The first, second and third authors contributed equally to this work.
Publisher Copyright:
© 2021 Elsevier B.V.
PY - 2021/8
Y1 - 2021/8
N2 - In recent years, obesity has become a major problem in the western world, affecting almost one-third of the population. Treatment for obesity is very expensive; therefore, the ‘fat-busting’ hormone leptin (LEP) has provoked wide-ranging interest over the last few years, and the immunosensing of LEP has received considerable attention in the field of obesity diagnosis. Bimetal oxides are significantly promising materials for biosensor applications owing to their large surface area, high stability, many active sites, and good biocompatibility. In this study, cerium niobate/cerium oxide (Ce3NbO7/CeO2) hollow nanospheres were synthesized using a template-free hydrothermal technique. The Ce3NbO7/CeO2 exhibits the high catalytic activity, large surface area, porous nature, and more active sites towards the LEP immunosensor. To improve the conductivity of the electrode, the AuNPs were decorated through the electrodeposition method (potential range: -0.4 to -0.9 V with an optimum successive cycle of 20). Au/Ce3NbO7/CeO2 was then thiol factionalized (Au-S bond-forming) with MPA to obtain a strong surface. Consequently, EDC/NHS enabled the −COOH group activation and firmly bonded with the anti-LEP molecules. Under optimal conditions, the developed immunosensor exhibited a good linear range from 0.5 pg/mL to 12,000 ± 500 pg/mL with a low detection limit (LOD) of 0.138 pg/mL, and good precision, selectivity, and stability results achieved for the immunosensing of LEP. Furthermore, the practical feasibility of LEP was demonstrated in plasma samples.
AB - In recent years, obesity has become a major problem in the western world, affecting almost one-third of the population. Treatment for obesity is very expensive; therefore, the ‘fat-busting’ hormone leptin (LEP) has provoked wide-ranging interest over the last few years, and the immunosensing of LEP has received considerable attention in the field of obesity diagnosis. Bimetal oxides are significantly promising materials for biosensor applications owing to their large surface area, high stability, many active sites, and good biocompatibility. In this study, cerium niobate/cerium oxide (Ce3NbO7/CeO2) hollow nanospheres were synthesized using a template-free hydrothermal technique. The Ce3NbO7/CeO2 exhibits the high catalytic activity, large surface area, porous nature, and more active sites towards the LEP immunosensor. To improve the conductivity of the electrode, the AuNPs were decorated through the electrodeposition method (potential range: -0.4 to -0.9 V with an optimum successive cycle of 20). Au/Ce3NbO7/CeO2 was then thiol factionalized (Au-S bond-forming) with MPA to obtain a strong surface. Consequently, EDC/NHS enabled the −COOH group activation and firmly bonded with the anti-LEP molecules. Under optimal conditions, the developed immunosensor exhibited a good linear range from 0.5 pg/mL to 12,000 ± 500 pg/mL with a low detection limit (LOD) of 0.138 pg/mL, and good precision, selectivity, and stability results achieved for the immunosensing of LEP. Furthermore, the practical feasibility of LEP was demonstrated in plasma samples.
KW - CeNbO/CeO
KW - Electrochemical immunosensor
KW - Hollow nanospheres
KW - Leptin
KW - Obese hormone
KW - Template-free
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U2 - 10.1016/j.snb.2021.130005
DO - 10.1016/j.snb.2021.130005
M3 - Article
AN - SCOPUS:85104636306
SN - 0925-4005
VL - 341
JO - Sensors and Actuators, B: Chemical
JF - Sensors and Actuators, B: Chemical
M1 - 130005
ER -