Tc17 CD8 T cells: Functional plasticity and subset diversity

Hung Rong Yen; Timothy J. Harris; Satoshi Wada; Joseph F. Grosso; Derese Getnet; Monica V. Goldberg; Kai Li Liang; Tullia C. Bruno; Kristin J. Pyle; Siaw Li Chan; Robert A. Anders; Cornelia L. Trimble; Adam J. Adler; Tzou Yien Lin; Drew M. Pardoll; Ching Tai Huang; Charles G. Drake

doi:10.4049/jimmunol.0900368

Tc17 CD8 T cells: Functional plasticity and subset diversity

Hung Rong Yen, Timothy J. Harris, Satoshi Wada, Joseph F. Grosso, Derese Getnet, Monica V. Goldberg, Kai Li Liang, Tullia C. Bruno, Kristin J. Pyle, Siaw Li Chan, Robert A. Anders, Cornelia L. Trimble, Adam J. Adler, Tzou Yien Lin, Drew M. Pardoll, Ching Tai Huang, Charles G. Drake

Research output: Contribution to journal › Article › peer-review

167 Citations (Scopus)

Abstract

IL-17-secreting CD8 T cells (Tc17) have been described in several settings, but little is known regarding their functional characteristics. While Tc1 cells produced IFN-γ and efficiently killed targets, Tc17 cells lacked lytic function in vitro. Interestingly, the small numbers of IFN-γ-positive or IL-17/IFN-γ-double-positive cells generated under Tc17 conditions also lacked lytic activity and expressed a similar pattern of cell surface proteins to IL-17-producing cells. As is the case for Th17 (CD4) cells, STAT3 is important for Tc17 polarization, both in vitro and in vivo. Adoptive transfer of highly purified, Ag-specific IL-17-secreting Tc17 cells into Ag-bearing hosts resulted in near complete conversion to an IFN-γ-secreting phenotype and substantial pulmonary pathology, demonstrating functional plasticity. Tc17 also accumulated to a greater extent than did Tc1 cells, suggesting that adoptive transfer of CD8 T cells cultured in Tc17 conditions may have therapeutic potential for diseases in which IFN-γ-producing cells are desired.

Original language	English
Pages (from-to)	7161-7168
Number of pages	8
Journal	Journal of Immunology
Volume	183
Issue number	11
DOIs	https://doi.org/10.4049/jimmunol.0900368
Publication status	Published - Dec 1 2009
Externally published	Yes

ASJC Scopus subject areas

Immunology
General Medicine

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Yen, H. R., Harris, T. J., Wada, S., Grosso, J. F., Getnet, D., Goldberg, M. V., Liang, K. L., Bruno, T. C., Pyle, K. J., Chan, S. L., Anders, R. A., Trimble, C. L., Adler, A. J., Lin, T. Y., Pardoll, D. M., Huang, C. T., & Drake, C. G. (2009). Tc17 CD8 T cells: Functional plasticity and subset diversity. Journal of Immunology, 183(11), 7161-7168. https://doi.org/10.4049/jimmunol.0900368

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title = "Tc17 CD8 T cells: Functional plasticity and subset diversity",

abstract = "IL-17-secreting CD8 T cells (Tc17) have been described in several settings, but little is known regarding their functional characteristics. While Tc1 cells produced IFN-γ and efficiently killed targets, Tc17 cells lacked lytic function in vitro. Interestingly, the small numbers of IFN-γ-positive or IL-17/IFN-γ-double-positive cells generated under Tc17 conditions also lacked lytic activity and expressed a similar pattern of cell surface proteins to IL-17-producing cells. As is the case for Th17 (CD4) cells, STAT3 is important for Tc17 polarization, both in vitro and in vivo. Adoptive transfer of highly purified, Ag-specific IL-17-secreting Tc17 cells into Ag-bearing hosts resulted in near complete conversion to an IFN-γ-secreting phenotype and substantial pulmonary pathology, demonstrating functional plasticity. Tc17 also accumulated to a greater extent than did Tc1 cells, suggesting that adoptive transfer of CD8 T cells cultured in Tc17 conditions may have therapeutic potential for diseases in which IFN-γ-producing cells are desired.",

author = "Yen, {Hung Rong} and Harris, {Timothy J.} and Satoshi Wada and Grosso, {Joseph F.} and Derese Getnet and Goldberg, {Monica V.} and Liang, {Kai Li} and Bruno, {Tullia C.} and Pyle, {Kristin J.} and Chan, {Siaw Li} and Anders, {Robert A.} and Trimble, {Cornelia L.} and Adler, {Adam J.} and Lin, {Tzou Yien} and Pardoll, {Drew M.} and Huang, {Ching Tai} and Drake, {Charles G.}",

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AU - Yen, Hung Rong

AU - Harris, Timothy J.

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AU - Getnet, Derese

AU - Goldberg, Monica V.

AU - Liang, Kai Li

AU - Bruno, Tullia C.

AU - Pyle, Kristin J.

AU - Chan, Siaw Li

AU - Anders, Robert A.

AU - Trimble, Cornelia L.

AU - Adler, Adam J.

AU - Lin, Tzou Yien

AU - Pardoll, Drew M.

AU - Huang, Ching Tai

AU - Drake, Charles G.

PY - 2009/12/1

Y1 - 2009/12/1

N2 - IL-17-secreting CD8 T cells (Tc17) have been described in several settings, but little is known regarding their functional characteristics. While Tc1 cells produced IFN-γ and efficiently killed targets, Tc17 cells lacked lytic function in vitro. Interestingly, the small numbers of IFN-γ-positive or IL-17/IFN-γ-double-positive cells generated under Tc17 conditions also lacked lytic activity and expressed a similar pattern of cell surface proteins to IL-17-producing cells. As is the case for Th17 (CD4) cells, STAT3 is important for Tc17 polarization, both in vitro and in vivo. Adoptive transfer of highly purified, Ag-specific IL-17-secreting Tc17 cells into Ag-bearing hosts resulted in near complete conversion to an IFN-γ-secreting phenotype and substantial pulmonary pathology, demonstrating functional plasticity. Tc17 also accumulated to a greater extent than did Tc1 cells, suggesting that adoptive transfer of CD8 T cells cultured in Tc17 conditions may have therapeutic potential for diseases in which IFN-γ-producing cells are desired.

AB - IL-17-secreting CD8 T cells (Tc17) have been described in several settings, but little is known regarding their functional characteristics. While Tc1 cells produced IFN-γ and efficiently killed targets, Tc17 cells lacked lytic function in vitro. Interestingly, the small numbers of IFN-γ-positive or IL-17/IFN-γ-double-positive cells generated under Tc17 conditions also lacked lytic activity and expressed a similar pattern of cell surface proteins to IL-17-producing cells. As is the case for Th17 (CD4) cells, STAT3 is important for Tc17 polarization, both in vitro and in vivo. Adoptive transfer of highly purified, Ag-specific IL-17-secreting Tc17 cells into Ag-bearing hosts resulted in near complete conversion to an IFN-γ-secreting phenotype and substantial pulmonary pathology, demonstrating functional plasticity. Tc17 also accumulated to a greater extent than did Tc1 cells, suggesting that adoptive transfer of CD8 T cells cultured in Tc17 conditions may have therapeutic potential for diseases in which IFN-γ-producing cells are desired.

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Tc17 CD8 T cells: Functional plasticity and subset diversity

Abstract

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