Abstract
Tannic acid (TA) is a polyphenol compound present in wines and many beverages. Although previous works have shown that TA could cause vasodilation in an endothelial cell (EC)-dependent manner, there is hitherto no report showing whether TA could raise EC cytosolic Ca2+ concentration. In this work we examined the effects of TA on cytosolic Ca2+ of mouse brain bEND.3 EC. TA (1–30 μM) caused a slow elevation in cytosolic Ca2+ level in a concentration-dependent manner. At 30 μM, TA triggered Ca2+ influx without causing intracellular Ca2+ release. TA-triggered Ca2+ influx was suppressed by Ni2+ (a non-specific Ca2+ channel blocker), ruthenium red and SKF 96365 (non-specific TRP channel blockers), CBA (a selective TRPM4 inhibitor) and M 084 (a selective TRPC4/C5 blocker). However, TA-triggered Ca2+ influx pathway was not permeable to Mn2+. Our results suggest TA activated TRP channels, possibly TRPM4 and TRPC4/C5, to promote influx of Ca2+.
Original language | English |
---|---|
Pages (from-to) | 117-121 |
Number of pages | 5 |
Journal | Biochemical and Biophysical Research Communications |
Volume | 526 |
Issue number | 1 |
DOIs | |
Publication status | Published - May 21 2020 |
Keywords
- Ca
- CBA
- Endothelial cells
- M 084
- Ruthenium red
- SKF96365
- Tannic acid
ASJC Scopus subject areas
- Biophysics
- Biochemistry
- Molecular Biology
- Cell Biology