T-type calcium current in electrical activity of cardiomyocytes isolated from rabbit pulmonary vein

Yao Chang Chen; Shih Ann Chen; Yi Jen Chen; Ching Tai Tai; Paul Chan; Cheng I. Lin

doi:10.1046/j.1540-8167.2004.03399.x

T-type calcium current in electrical activity of cardiomyocytes isolated from rabbit pulmonary vein

Yao Chang Chen, Shih Ann Chen, Yi Jen Chen, Ching Tai Tai, Paul Chan, Cheng I. Lin

Research output: Contribution to journal › Article › peer-review

49 Citations (Scopus)

Abstract

Introduction: Pulmonary veins (PVs) are known to initiate paroxysmal atrial fibrillation. T-type calcium current (I_Ca-T) has a role in normal and abnormal automaticity of cardiomyocytes. The aim of this study was to evaluate whether I_Ca-T contributes to PV electrical activity. Methods and Results: By whole-cell clamp techniques in rabbit myocytes, I _Ca-T was identified in 12 (39%) of 31 PV cardiomyocytes with pacemaker activity, 2 (9%) of 23 PV cardiomyocytes without pacemaker activity, and 2 (15%) of 13 atrial myocytes (P < 0.05). Maximum I_Ca-L and I_Ca-T densities from PV cardiomyocytes with pacemaker activity were 6.87 ± 2.17 pA/pF and 1.38 ± 0.69 pA/pF, respectively. Nickel (40 μM) decreased the spontaneous activity in 5 (36%) of 14 PV cardiomyocytes (3.1 ± 0.6 Hz vs 2.2 ± 0.5 Hz, P < 0.05), reduced the amplitudes of delayed afterdepolarization from 13 ± 1 mV to 7 ± 1 mV (n = 4, P < 0.05) and inhibited transient inward currents from 1.2 ± 0.2 pA/pF to 0.7 ± 0.1 pA/pF (n = 11, P < 0.01). Conclusions: We conclude that I_Ca-T contributes to PV pacemaker activity and triggered activity, which are of functional importance in PV arrhythmogenesis.

Original language	English
Pages (from-to)	567-571
Number of pages	5
Journal	Journal of Cardiovascular Electrophysiology
Volume	15
Issue number	5
DOIs	https://doi.org/10.1046/j.1540-8167.2004.03399.x
Publication status	Published - May 2004

Keywords

Calcium channel
Nickel
Pulmonary vein
Transient inward current

ASJC Scopus subject areas

Cardiology and Cardiovascular Medicine
Physiology (medical)

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10.1046/j.1540-8167.2004.03399.x

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@article{874c8ed297b141df95656dd45360696e,

title = "T-type calcium current in electrical activity of cardiomyocytes isolated from rabbit pulmonary vein",

abstract = "Introduction: Pulmonary veins (PVs) are known to initiate paroxysmal atrial fibrillation. T-type calcium current (ICa-T) has a role in normal and abnormal automaticity of cardiomyocytes. The aim of this study was to evaluate whether ICa-T contributes to PV electrical activity. Methods and Results: By whole-cell clamp techniques in rabbit myocytes, I Ca-T was identified in 12 (39%) of 31 PV cardiomyocytes with pacemaker activity, 2 (9%) of 23 PV cardiomyocytes without pacemaker activity, and 2 (15%) of 13 atrial myocytes (P < 0.05). Maximum ICa-L and ICa-T densities from PV cardiomyocytes with pacemaker activity were 6.87 ± 2.17 pA/pF and 1.38 ± 0.69 pA/pF, respectively. Nickel (40 μM) decreased the spontaneous activity in 5 (36%) of 14 PV cardiomyocytes (3.1 ± 0.6 Hz vs 2.2 ± 0.5 Hz, P < 0.05), reduced the amplitudes of delayed afterdepolarization from 13 ± 1 mV to 7 ± 1 mV (n = 4, P < 0.05) and inhibited transient inward currents from 1.2 ± 0.2 pA/pF to 0.7 ± 0.1 pA/pF (n = 11, P < 0.01). Conclusions: We conclude that ICa-T contributes to PV pacemaker activity and triggered activity, which are of functional importance in PV arrhythmogenesis.",

keywords = "Calcium channel, Nickel, Pulmonary vein, Transient inward current",

author = "Chen, {Yao Chang} and Chen, {Shih Ann} and Chen, {Yi Jen} and Tai, {Ching Tai} and Paul Chan and Lin, {Cheng I.}",

year = "2004",

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doi = "10.1046/j.1540-8167.2004.03399.x",

language = "English",

volume = "15",

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T1 - T-type calcium current in electrical activity of cardiomyocytes isolated from rabbit pulmonary vein

AU - Chen, Yao Chang

AU - Chen, Shih Ann

AU - Chen, Yi Jen

AU - Tai, Ching Tai

AU - Chan, Paul

AU - Lin, Cheng I.

PY - 2004/5

Y1 - 2004/5

N2 - Introduction: Pulmonary veins (PVs) are known to initiate paroxysmal atrial fibrillation. T-type calcium current (ICa-T) has a role in normal and abnormal automaticity of cardiomyocytes. The aim of this study was to evaluate whether ICa-T contributes to PV electrical activity. Methods and Results: By whole-cell clamp techniques in rabbit myocytes, I Ca-T was identified in 12 (39%) of 31 PV cardiomyocytes with pacemaker activity, 2 (9%) of 23 PV cardiomyocytes without pacemaker activity, and 2 (15%) of 13 atrial myocytes (P < 0.05). Maximum ICa-L and ICa-T densities from PV cardiomyocytes with pacemaker activity were 6.87 ± 2.17 pA/pF and 1.38 ± 0.69 pA/pF, respectively. Nickel (40 μM) decreased the spontaneous activity in 5 (36%) of 14 PV cardiomyocytes (3.1 ± 0.6 Hz vs 2.2 ± 0.5 Hz, P < 0.05), reduced the amplitudes of delayed afterdepolarization from 13 ± 1 mV to 7 ± 1 mV (n = 4, P < 0.05) and inhibited transient inward currents from 1.2 ± 0.2 pA/pF to 0.7 ± 0.1 pA/pF (n = 11, P < 0.01). Conclusions: We conclude that ICa-T contributes to PV pacemaker activity and triggered activity, which are of functional importance in PV arrhythmogenesis.

AB - Introduction: Pulmonary veins (PVs) are known to initiate paroxysmal atrial fibrillation. T-type calcium current (ICa-T) has a role in normal and abnormal automaticity of cardiomyocytes. The aim of this study was to evaluate whether ICa-T contributes to PV electrical activity. Methods and Results: By whole-cell clamp techniques in rabbit myocytes, I Ca-T was identified in 12 (39%) of 31 PV cardiomyocytes with pacemaker activity, 2 (9%) of 23 PV cardiomyocytes without pacemaker activity, and 2 (15%) of 13 atrial myocytes (P < 0.05). Maximum ICa-L and ICa-T densities from PV cardiomyocytes with pacemaker activity were 6.87 ± 2.17 pA/pF and 1.38 ± 0.69 pA/pF, respectively. Nickel (40 μM) decreased the spontaneous activity in 5 (36%) of 14 PV cardiomyocytes (3.1 ± 0.6 Hz vs 2.2 ± 0.5 Hz, P < 0.05), reduced the amplitudes of delayed afterdepolarization from 13 ± 1 mV to 7 ± 1 mV (n = 4, P < 0.05) and inhibited transient inward currents from 1.2 ± 0.2 pA/pF to 0.7 ± 0.1 pA/pF (n = 11, P < 0.01). Conclusions: We conclude that ICa-T contributes to PV pacemaker activity and triggered activity, which are of functional importance in PV arrhythmogenesis.

KW - Calcium channel

KW - Nickel

KW - Pulmonary vein

KW - Transient inward current

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ER -

T-type calcium current in electrical activity of cardiomyocytes isolated from rabbit pulmonary vein

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