Synthesis of polysaccharide scaffolds with bioactive substances for tissue engineering

Based on polysaccharide materials, chitosan-alginate polyelectrolyte complex, are widely used on tissue engineering for wound repair and tissue reconstruction. The goal of study is to combine bioactive substances, basic fibroblast growth factor (bFGF) and mice epidermal growth factor (mEGF), into polysaccharide scaffolds, which serve as controlled-release growth factors carriers to induce vascularization and mediate fibroblast migration. The result indicate that release rate of bFGF could be controlled by varying the pH of alginate solution in the initial incorporation content of bFGF. The revulsive tissue reconstruction of these polysaccharide scaffolds in vivo was evaluated by histological H&E stain and DSC analysis after implantation in SD rat. The vascular was aplenty generated surrounding the bFGF incorporation polysaccharide scaffolds after 1 week of implantation. At 3-week post-operation, new synthesis of collagen was found inside the scaffold, but non-loading bFGF scaffolds were not detected. Further more, the scaffold will be biolysis and replacement by new tissue. All experiment shows that polysaccharide scaffolds incorporate with bFGF may be beneficial to enhance vascularization in the initial time and regeneration new tissue in last stage, which has potential to apply in tissue engineering field.