Synthesis and characterization of Gd-DTPA/fucoidan/peptide complex nanoparticle and in vitro magnetic resonance imaging of inflamed endothelial cells

Tsai Mu Cheng; Rou Li; Yu Chieh Jill Kao; Chun Hua Hsu; Hsueh Liang Chu; Kun Ying Lu; Chun A. Changou; Che Chang Chang; Lee Hsin Chang; Min Lang Tsai; Fwu Long Mi

doi:10.1016/j.msec.2020.111064

Synthesis and characterization of Gd-DTPA/fucoidan/peptide complex nanoparticle and in vitro magnetic resonance imaging of inflamed endothelial cells

Tsai Mu Cheng, Rou Li, Yu Chieh Jill Kao, Chun Hua Hsu, Hsueh Liang Chu, Kun Ying Lu, Chun A. Changou, Che Chang Chang, Lee Hsin Chang, Min Lang Tsai, Fwu Long Mi

Research output: Contribution to journal › Article › peer-review

29 Citations (Scopus)

Abstract

P-selectin overexpressed on activated endothelial cells and platelets is a new target for treatment of cancers and cardiovascular diseases such as atherosclerosis and thrombosis. In this study, depolymerized low molecular weight fucoidan (LMWF₈₇₇₅) and a thermolysin-hydrolyzed protamine peptide (TPP₁₈₈₀) were prepared. TPP₁₈₈₀ and LMWF₈₇₇₅ were able to form self-assembled complex nanoparticles (CNPs). The formation of TPP₁₈₈₀/LMWF₈₇₇₅ CNPs was characterized by Fourier-transform infrared spectra, circular dichroism spectra and isothermal titration calorimetry. The CNPs selectively targeted PMA-stimulated, inflamed endothelial cells (HUVECs) with high expression of P-selectin. Gd-DTPA MRI contrast agent was successfully loaded in the CNPs with better T₁ relaxivity and selectively accumulated in the activated HUVECs with increased MRI intensity and reduced cytotoxicity as compared to free Gd-DTPA. Our results suggest that the TPP₁₈₈₀/LMWF₈₇₇₅ CNPs may have potential in future for early diagnosis of cardiovascular diseases and cancers in which the endothelium is inflamed or activated.

Original language	English
Article number	111064
Journal	Materials Science and Engineering C
Volume	114
DOIs	https://doi.org/10.1016/j.msec.2020.111064
Publication status	Published - Sept 2020

Keywords

Cell penetrating peptide
Drug delivery
Fucoidan
Imaging
Nanoparticles
Targeting

ASJC Scopus subject areas

General Materials Science
Condensed Matter Physics
Mechanics of Materials
Mechanical Engineering

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.msec.2020.111064

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Cheng, T. M., Li, R., Kao, Y. C. J., Hsu, C. H., Chu, H. L., Lu, K. Y., Changou, C. A., Chang, C. C., Chang, L. H., Tsai, M. L., & Mi, F. L. (2020). Synthesis and characterization of Gd-DTPA/fucoidan/peptide complex nanoparticle and in vitro magnetic resonance imaging of inflamed endothelial cells. Materials Science and Engineering C, 114, Article 111064. https://doi.org/10.1016/j.msec.2020.111064

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title = "Synthesis and characterization of Gd-DTPA/fucoidan/peptide complex nanoparticle and in vitro magnetic resonance imaging of inflamed endothelial cells",

abstract = "P-selectin overexpressed on activated endothelial cells and platelets is a new target for treatment of cancers and cardiovascular diseases such as atherosclerosis and thrombosis. In this study, depolymerized low molecular weight fucoidan (LMWF8775) and a thermolysin-hydrolyzed protamine peptide (TPP1880) were prepared. TPP1880 and LMWF8775 were able to form self-assembled complex nanoparticles (CNPs). The formation of TPP1880/LMWF8775 CNPs was characterized by Fourier-transform infrared spectra, circular dichroism spectra and isothermal titration calorimetry. The CNPs selectively targeted PMA-stimulated, inflamed endothelial cells (HUVECs) with high expression of P-selectin. Gd-DTPA MRI contrast agent was successfully loaded in the CNPs with better T1 relaxivity and selectively accumulated in the activated HUVECs with increased MRI intensity and reduced cytotoxicity as compared to free Gd-DTPA. Our results suggest that the TPP1880/LMWF8775 CNPs may have potential in future for early diagnosis of cardiovascular diseases and cancers in which the endothelium is inflamed or activated.",

keywords = "Cell penetrating peptide, Drug delivery, Fucoidan, Imaging, Nanoparticles, Targeting",

author = "Cheng, {Tsai Mu} and Rou Li and Kao, {Yu Chieh Jill} and Hsu, {Chun Hua} and Chu, {Hsueh Liang} and Lu, {Kun Ying} and Changou, {Chun A.} and Chang, {Che Chang} and Chang, {Lee Hsin} and Tsai, {Min Lang} and Mi, {Fwu Long}",

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year = "2020",

month = sep,

doi = "10.1016/j.msec.2020.111064",

language = "English",

volume = "114",

journal = "Materials Science and Engineering C",

issn = "0928-4931",

publisher = "Elsevier BV",

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TY - JOUR

T1 - Synthesis and characterization of Gd-DTPA/fucoidan/peptide complex nanoparticle and in vitro magnetic resonance imaging of inflamed endothelial cells

AU - Cheng, Tsai Mu

AU - Li, Rou

AU - Kao, Yu Chieh Jill

AU - Hsu, Chun Hua

AU - Chu, Hsueh Liang

AU - Lu, Kun Ying

AU - Changou, Chun A.

AU - Chang, Che Chang

AU - Chang, Lee Hsin

AU - Tsai, Min Lang

AU - Mi, Fwu Long

PY - 2020/9

Y1 - 2020/9

N2 - P-selectin overexpressed on activated endothelial cells and platelets is a new target for treatment of cancers and cardiovascular diseases such as atherosclerosis and thrombosis. In this study, depolymerized low molecular weight fucoidan (LMWF8775) and a thermolysin-hydrolyzed protamine peptide (TPP1880) were prepared. TPP1880 and LMWF8775 were able to form self-assembled complex nanoparticles (CNPs). The formation of TPP1880/LMWF8775 CNPs was characterized by Fourier-transform infrared spectra, circular dichroism spectra and isothermal titration calorimetry. The CNPs selectively targeted PMA-stimulated, inflamed endothelial cells (HUVECs) with high expression of P-selectin. Gd-DTPA MRI contrast agent was successfully loaded in the CNPs with better T1 relaxivity and selectively accumulated in the activated HUVECs with increased MRI intensity and reduced cytotoxicity as compared to free Gd-DTPA. Our results suggest that the TPP1880/LMWF8775 CNPs may have potential in future for early diagnosis of cardiovascular diseases and cancers in which the endothelium is inflamed or activated.

AB - P-selectin overexpressed on activated endothelial cells and platelets is a new target for treatment of cancers and cardiovascular diseases such as atherosclerosis and thrombosis. In this study, depolymerized low molecular weight fucoidan (LMWF8775) and a thermolysin-hydrolyzed protamine peptide (TPP1880) were prepared. TPP1880 and LMWF8775 were able to form self-assembled complex nanoparticles (CNPs). The formation of TPP1880/LMWF8775 CNPs was characterized by Fourier-transform infrared spectra, circular dichroism spectra and isothermal titration calorimetry. The CNPs selectively targeted PMA-stimulated, inflamed endothelial cells (HUVECs) with high expression of P-selectin. Gd-DTPA MRI contrast agent was successfully loaded in the CNPs with better T1 relaxivity and selectively accumulated in the activated HUVECs with increased MRI intensity and reduced cytotoxicity as compared to free Gd-DTPA. Our results suggest that the TPP1880/LMWF8775 CNPs may have potential in future for early diagnosis of cardiovascular diseases and cancers in which the endothelium is inflamed or activated.

KW - Cell penetrating peptide

KW - Drug delivery

KW - Fucoidan

KW - Imaging

KW - Nanoparticles

KW - Targeting

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Synthesis and characterization of Gd-DTPA/fucoidan/peptide complex nanoparticle and in vitro magnetic resonance imaging of inflamed endothelial cells

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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