TY - JOUR
T1 - Synergistic integration of Netrin and ephrin axon guidance signals by spinal motor neurons
AU - Poliak, Sebastian
AU - Morales, Daniel
AU - Croteau, Louis-Philippe
AU - Krawchuk, Dayana
AU - Palmesino, Elena
AU - Morton, Susan
AU - Cloutier, Jean-François
AU - Charron, Frederic
AU - Dalva, Matthew B
AU - Ackerman, Susan L
AU - Kao, Tzu-Jen
AU - Kania, Artur
PY - 2015
Y1 - 2015
N2 - During neural circuit assembly, axonal growth cones are exposed to multiple guidance signals at trajectory choice points. While axonal responses to individual guidance cues have been extensively studied, less is known about responses to combination of signals and underlying molecular mechanisms. Here, we studied the convergence of signals directing trajectory selection of spinal motor axons entering the limb. We first demonstrate that Netrin-1 attracts and repels distinct motor axon populations, according to their expression of Netrin receptors. Quantitative in vitro assays demonstrate that motor axons synergistically integrate both attractive or repulsive Netrin-1 signals together with repulsive ephrin signals. Our investigations of the mechanism of ephrin-B2 and Netrin-1 integration demonstrate that the Netrin receptor Unc5c and the ephrin receptor EphB2 can form a complex in a ligand-dependent manner and that Netrin-ephrin synergistic growth cones responses involve the potentiation of Src family kinase signaling, a common effector of both pathways.
AB - During neural circuit assembly, axonal growth cones are exposed to multiple guidance signals at trajectory choice points. While axonal responses to individual guidance cues have been extensively studied, less is known about responses to combination of signals and underlying molecular mechanisms. Here, we studied the convergence of signals directing trajectory selection of spinal motor axons entering the limb. We first demonstrate that Netrin-1 attracts and repels distinct motor axon populations, according to their expression of Netrin receptors. Quantitative in vitro assays demonstrate that motor axons synergistically integrate both attractive or repulsive Netrin-1 signals together with repulsive ephrin signals. Our investigations of the mechanism of ephrin-B2 and Netrin-1 integration demonstrate that the Netrin receptor Unc5c and the ephrin receptor EphB2 can form a complex in a ligand-dependent manner and that Netrin-ephrin synergistic growth cones responses involve the potentiation of Src family kinase signaling, a common effector of both pathways.
KW - Journal Article
KW - Research Support, Non-U.S. Gov't
UR - https://www.scopus.com/record/display.uri?eid=2-s2.0-84955613323&origin=resultslist&sort=plf-f&src=s&sid=c835561d8652e128218d71638f367476&sot=a&sdt=a&sl=18&s=AU-ID%2826537500800%29&relpos=6&citeCnt=16&searchTerm=
UR - https://www.scopus.com/results/citedbyresults.uri?sort=plf-f&cite=2-s2.0-84955613323&src=s&imp=t&sid=3679de420951f4cc3c1d9aeb8e0fc1e1&sot=cite&sdt=a&sl=0&origin=recordpage&editSaveSearch=&txGid=7fba617de9ec8f645ad25cbf119f44be
U2 - 10.7554/eLife.10841
DO - 10.7554/eLife.10841
M3 - Article
C2 - 26633881
SN - 2050-084X
VL - 4
JO - eLife
JF - eLife
ER -