Synergistic effect of HIF-1α and FoxO3a trigger cardiomyocyte apoptosis under hyperglycemic ischemia condition

Ya Fang Chen; Sudhir Pandey; Cecilia Hsuan Day; Yu Feng Chen; Ai Zhi Jiang; Tsung Jung Ho; Ray Jade Chen; Vijaya V. Padma; Wei Wen Kuo; Chih Yang Huang

doi:10.1002/jcp.26235

Synergistic effect of HIF-1α and FoxO3a trigger cardiomyocyte apoptosis under hyperglycemic ischemia condition

Ya Fang Chen, Sudhir Pandey, Cecilia Hsuan Day, Yu Feng Chen, Ai Zhi Jiang, Tsung Jung Ho, Ray Jade Chen, Vijaya V. Padma, Wei Wen Kuo, Chih Yang Huang

Research output: Contribution to journal › Article › peer-review

53 Citations (Scopus)

Abstract

Cardiomyocyte death is an important pathogenic feature of ischemia and heart failure. Through this study, we showed the synergistic role of HIF-1α and FoxO3a in cardiomyocyte apoptosis subjected to hypoxia plus elevated glucose levels. Using gene specific small interfering RNAs (siRNA), semi-quantitative reverse transcriptase polymerase chain reaction (RT-PCR), Western blot, immunofluorescence, nuclear and cytosolic localization and TUNEL assay techniques, we determined that combined function of HIF-1α and FoxO3a under high glucose plus hypoxia condition lead to enhanced expression of BNIP3 inducing cardiomyocyte death. Our results highlighted the importance of the synergistic role of HIF-1α and FoxO3a in cardiomyocyte death which may add insight into therapeutic approaches to pathophysiology associated with ischemic diabetic cardiomyopathies.

Original language	English
Pages (from-to)	3660-3671
Number of pages	12
Journal	Journal of Cellular Physiology
Volume	233
Issue number	4
DOIs	https://doi.org/10.1002/jcp.26235
Publication status	Published - Apr 2018

Keywords

BCL2/Adenovirus E1B Nineteen kilo Dalton Interacting Protein 3 (BNIP3)
diabetic hyperglycemic
forkhead box O3 (FoxO3)
hyperglycemia
hypoxia-inducible factor-1 alpha (HIF-1α)
ischemia hypoxia

ASJC Scopus subject areas

Physiology
Clinical Biochemistry
Cell Biology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1002/jcp.26235

Cite this

@article{80fa4cfe1fb745ac9e8c424f8bf75181,

title = "Synergistic effect of HIF-1α and FoxO3a trigger cardiomyocyte apoptosis under hyperglycemic ischemia condition",

abstract = "Cardiomyocyte death is an important pathogenic feature of ischemia and heart failure. Through this study, we showed the synergistic role of HIF-1α and FoxO3a in cardiomyocyte apoptosis subjected to hypoxia plus elevated glucose levels. Using gene specific small interfering RNAs (siRNA), semi-quantitative reverse transcriptase polymerase chain reaction (RT-PCR), Western blot, immunofluorescence, nuclear and cytosolic localization and TUNEL assay techniques, we determined that combined function of HIF-1α and FoxO3a under high glucose plus hypoxia condition lead to enhanced expression of BNIP3 inducing cardiomyocyte death. Our results highlighted the importance of the synergistic role of HIF-1α and FoxO3a in cardiomyocyte death which may add insight into therapeutic approaches to pathophysiology associated with ischemic diabetic cardiomyopathies.",

keywords = "BCL2/Adenovirus E1B Nineteen kilo Dalton Interacting Protein 3 (BNIP3), diabetic hyperglycemic, forkhead box O3 (FoxO3), hyperglycemia, hypoxia-inducible factor-1 alpha (HIF-1α), ischemia hypoxia",

author = "Chen, {Ya Fang} and Sudhir Pandey and Day, {Cecilia Hsuan} and Chen, {Yu Feng} and Jiang, {Ai Zhi} and Ho, {Tsung Jung} and Chen, {Ray Jade} and Padma, {Vijaya V.} and Kuo, {Wei Wen} and Huang, {Chih Yang}",

note = "Publisher Copyright: {\textcopyright} 2017 Wiley Periodicals, Inc.",

year = "2018",

month = apr,

doi = "10.1002/jcp.26235",

language = "English",

volume = "233",

pages = "3660--3671",

journal = "Journal of Cellular Physiology",

issn = "0021-9541",

publisher = "Wiley-Liss Inc.",

number = "4",

}

TY - JOUR

T1 - Synergistic effect of HIF-1α and FoxO3a trigger cardiomyocyte apoptosis under hyperglycemic ischemia condition

AU - Chen, Ya Fang

AU - Pandey, Sudhir

AU - Day, Cecilia Hsuan

AU - Chen, Yu Feng

AU - Jiang, Ai Zhi

AU - Ho, Tsung Jung

AU - Chen, Ray Jade

AU - Padma, Vijaya V.

AU - Kuo, Wei Wen

AU - Huang, Chih Yang

PY - 2018/4

Y1 - 2018/4

N2 - Cardiomyocyte death is an important pathogenic feature of ischemia and heart failure. Through this study, we showed the synergistic role of HIF-1α and FoxO3a in cardiomyocyte apoptosis subjected to hypoxia plus elevated glucose levels. Using gene specific small interfering RNAs (siRNA), semi-quantitative reverse transcriptase polymerase chain reaction (RT-PCR), Western blot, immunofluorescence, nuclear and cytosolic localization and TUNEL assay techniques, we determined that combined function of HIF-1α and FoxO3a under high glucose plus hypoxia condition lead to enhanced expression of BNIP3 inducing cardiomyocyte death. Our results highlighted the importance of the synergistic role of HIF-1α and FoxO3a in cardiomyocyte death which may add insight into therapeutic approaches to pathophysiology associated with ischemic diabetic cardiomyopathies.

AB - Cardiomyocyte death is an important pathogenic feature of ischemia and heart failure. Through this study, we showed the synergistic role of HIF-1α and FoxO3a in cardiomyocyte apoptosis subjected to hypoxia plus elevated glucose levels. Using gene specific small interfering RNAs (siRNA), semi-quantitative reverse transcriptase polymerase chain reaction (RT-PCR), Western blot, immunofluorescence, nuclear and cytosolic localization and TUNEL assay techniques, we determined that combined function of HIF-1α and FoxO3a under high glucose plus hypoxia condition lead to enhanced expression of BNIP3 inducing cardiomyocyte death. Our results highlighted the importance of the synergistic role of HIF-1α and FoxO3a in cardiomyocyte death which may add insight into therapeutic approaches to pathophysiology associated with ischemic diabetic cardiomyopathies.

KW - BCL2/Adenovirus E1B Nineteen kilo Dalton Interacting Protein 3 (BNIP3)

KW - diabetic hyperglycemic

KW - forkhead box O3 (FoxO3)

KW - hyperglycemia

KW - hypoxia-inducible factor-1 alpha (HIF-1α)

KW - ischemia hypoxia

UR - http://www.scopus.com/inward/record.url?scp=85034033600&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85034033600&partnerID=8YFLogxK

U2 - 10.1002/jcp.26235

DO - 10.1002/jcp.26235

M3 - Article

AN - SCOPUS:85034033600

SN - 0021-9541

VL - 233

SP - 3660

EP - 3671

JO - Journal of Cellular Physiology

JF - Journal of Cellular Physiology

IS - 4

ER -

Synergistic effect of HIF-1α and FoxO3a trigger cardiomyocyte apoptosis under hyperglycemic ischemia condition

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this