Synergistic Benefit of Adoptive T Cells in Combination With Chemoradiotherapy Against Metastatic Prostate Cancer Cells

Yun Chu Shen, Hung Jen Shih, Chi Chien Lin, Sheng Hsien Huang, Sheng Chuan Wu, Chi Hao Hsiao, Shao Chi Chiu, DER E.R.Y. Cho, Chin Pao Chang, Yueh Pan, Ping Hsiao Shih

Research output: Contribution to journalArticlepeer-review


BACKGROUND/AIM: Prostate cancer (PC) is one of the major diseases that affects male health and ranks as the second most frequent cancer in men worldwide. Although most newly-diagnosed PCs are well-differentiated tumors with a high cure probability, there are some patients with aggressive malignancies that show potential for recurrence and metastasis. Cytotoxic T lymphocytes are a specific immune effector cell population that mediates immune responses against cancer. MATERIALS AND METHODS: In the present study, the cytotoxicity of peripheral blood mononuclear cells (PBMCs)-derived γδ T cells and cytokine-induced killer (CIK) cells in combination with chemoradiotherapy against PC cells was evaluated using Alamar blue cell viability and cell membrane permeability assays. RESULTS: Advanced PC-3 cells, which were more resistant to docetaxel (Doc), also showed higher viability following pretreatment with radiation. The cell proliferation inhibition was significantly increased upon additional γδ T or CIK treatment. Furthermore, the proportion of apoptotic cells was significantly (p<0.05) increased in the Doc-γδ T cell co-treatment group as compared with the Doc or γδ T cell treated alone group. CONCLUSION: γδ T cell therapy may provide additional benefit compared to traditional chemoradiotherapy for PC treatment.

Original languageEnglish
Pages (from-to)3427-3434
Number of pages8
JournalAnticancer Research
Issue number7
Publication statusPublished - Jul 1 2022


  • adoptive T cell therapy
  • chemoradiotherapy
  • cytokine-induced killer T cell
  • cytotoxicity
  • Prostate cancer
  • T cell
  • γδ

ASJC Scopus subject areas

  • Oncology
  • Cancer Research


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